Effects of 5-azacytidine-2’- deoxycytidine on the proliferation, cell cycle, and apoptosis of fetal bovine fibroblast cells
Fetal bovine fibroblast cells (FBFCs) are widely used in cellular reprogramming and methylation. In this study, the effects of 5- azacytidine-2’- deoxycytidines (5-Aza-CdR), a DNA methylation inhibitor on the physiological characteristics of FBFCs were investigated. The FBFCs derived from a bovine f...
Saved in:
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Zhejiang University Press
2018-11-01
|
| Series: | 浙江大学学报. 农业与生命科学版 |
| Subjects: | |
| Online Access: | https://www.academax.com/doi/10.3785/j.issn.1008-9209.2017.08.212 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849419696947855360 |
|---|---|
| author | YU Mengfei WANG Wenlu YI Jianming |
| author_facet | YU Mengfei WANG Wenlu YI Jianming |
| author_sort | YU Mengfei |
| collection | DOAJ |
| description | Fetal bovine fibroblast cells (FBFCs) are widely used in cellular reprogramming and methylation. In this study, the effects of 5- azacytidine-2’- deoxycytidines (5-Aza-CdR), a DNA methylation inhibitor on the physiological characteristics of FBFCs were investigated. The FBFCs derived from a bovine fetus were treated with 5-Aza-CdR at different concentrations for 24, 48 and 72 h, respectively, to determine the changes of cell proliferation, viability, cell cycle distribution and apoptosis rates. The results demonstrated that 5- Aza- CdR treatment in the FBFCs resulted in a decrease of cell proliferation, cell viability, and an increase of total cell apoptosis in a dose-dependent manner. The best treatment strategy for 5-Aza-CdR was less than 0.1 μmol/L for 24 h, which showed minimal adverse effects on FBFCs. The above results indicate that the low dose DNA demethylation inhibitor treatment in FBFCs is beneficial for cell reprogramming. |
| format | Article |
| id | doaj-art-cc12086a7d7e4e6ab3d228c4f7c8d6e4 |
| institution | Kabale University |
| issn | 1008-9209 2097-5155 |
| language | English |
| publishDate | 2018-11-01 |
| publisher | Zhejiang University Press |
| record_format | Article |
| series | 浙江大学学报. 农业与生命科学版 |
| spelling | doaj-art-cc12086a7d7e4e6ab3d228c4f7c8d6e42025-08-20T03:32:00ZengZhejiang University Press浙江大学学报. 农业与生命科学版1008-92092097-51552018-11-014472773410.3785/j.issn.1008-9209.2017.08.21210089209Effects of 5-azacytidine-2’- deoxycytidine on the proliferation, cell cycle, and apoptosis of fetal bovine fibroblast cellsYU MengfeiWANG WenluYI JianmingFetal bovine fibroblast cells (FBFCs) are widely used in cellular reprogramming and methylation. In this study, the effects of 5- azacytidine-2’- deoxycytidines (5-Aza-CdR), a DNA methylation inhibitor on the physiological characteristics of FBFCs were investigated. The FBFCs derived from a bovine fetus were treated with 5-Aza-CdR at different concentrations for 24, 48 and 72 h, respectively, to determine the changes of cell proliferation, viability, cell cycle distribution and apoptosis rates. The results demonstrated that 5- Aza- CdR treatment in the FBFCs resulted in a decrease of cell proliferation, cell viability, and an increase of total cell apoptosis in a dose-dependent manner. The best treatment strategy for 5-Aza-CdR was less than 0.1 μmol/L for 24 h, which showed minimal adverse effects on FBFCs. The above results indicate that the low dose DNA demethylation inhibitor treatment in FBFCs is beneficial for cell reprogramming.https://www.academax.com/doi/10.3785/j.issn.1008-9209.2017.08.2125-azacytidine-2’-deoxycytidinescell proliferationcell cyclecell apoptosis |
| spellingShingle | YU Mengfei WANG Wenlu YI Jianming Effects of 5-azacytidine-2’- deoxycytidine on the proliferation, cell cycle, and apoptosis of fetal bovine fibroblast cells 浙江大学学报. 农业与生命科学版 5-azacytidine-2’-deoxycytidines cell proliferation cell cycle cell apoptosis |
| title | Effects of 5-azacytidine-2’- deoxycytidine on the proliferation, cell cycle, and apoptosis of fetal bovine fibroblast cells |
| title_full | Effects of 5-azacytidine-2’- deoxycytidine on the proliferation, cell cycle, and apoptosis of fetal bovine fibroblast cells |
| title_fullStr | Effects of 5-azacytidine-2’- deoxycytidine on the proliferation, cell cycle, and apoptosis of fetal bovine fibroblast cells |
| title_full_unstemmed | Effects of 5-azacytidine-2’- deoxycytidine on the proliferation, cell cycle, and apoptosis of fetal bovine fibroblast cells |
| title_short | Effects of 5-azacytidine-2’- deoxycytidine on the proliferation, cell cycle, and apoptosis of fetal bovine fibroblast cells |
| title_sort | effects of 5 azacytidine 2 deoxycytidine on the proliferation cell cycle and apoptosis of fetal bovine fibroblast cells |
| topic | 5-azacytidine-2’-deoxycytidines cell proliferation cell cycle cell apoptosis |
| url | https://www.academax.com/doi/10.3785/j.issn.1008-9209.2017.08.212 |
| work_keys_str_mv | AT yumengfei effectsof5azacytidine2deoxycytidineontheproliferationcellcycleandapoptosisoffetalbovinefibroblastcells AT wangwenlu effectsof5azacytidine2deoxycytidineontheproliferationcellcycleandapoptosisoffetalbovinefibroblastcells AT yijianming effectsof5azacytidine2deoxycytidineontheproliferationcellcycleandapoptosisoffetalbovinefibroblastcells |