The abscisic acid signaling negative regulator OsPP2C68 confers drought and salinity tolerance to rice
Abstract Clade A type 2 C protein phosphatases (PP2CAs) are core regulatory factors in the plant abscisic acid (ABA) signaling pathway. They play crucial roles in response to abiotic stress. However, a comprehensive understanding of the functions of individual members of rice PP2CA gene families rem...
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Nature Portfolio
2025-02-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-025-91226-2 |
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| author | Beilei Wang Yuping Luo Binxia Zhong Han Xu Fengping Wang Wen Li Meiqi Lin Juan Chen Liangbi Chen Manzhong Liang Xiaojun Dai |
| author_facet | Beilei Wang Yuping Luo Binxia Zhong Han Xu Fengping Wang Wen Li Meiqi Lin Juan Chen Liangbi Chen Manzhong Liang Xiaojun Dai |
| author_sort | Beilei Wang |
| collection | DOAJ |
| description | Abstract Clade A type 2 C protein phosphatases (PP2CAs) are core regulatory factors in the plant abscisic acid (ABA) signaling pathway. They play crucial roles in response to abiotic stress. However, a comprehensive understanding of the functions of individual members of rice PP2CA gene families remains limited. This study investigates the role of OsPP2C68 in response to abiotic stress. Our findings indicated that the OsPP2C68 is highly expressed in the embryo and endosperm, and is subcellular localized in the nucleus. OsPP2C68 knockout mutants reduced seed germination and root and stem lengths under ABA treatment. The mutants also exhibited higher stomatal closure rates, indicating increased sensitivity to ABA. In addition, the OsPP2C68 knockout mutants exhibited altered synthesis of osmolytes and antioxidant enzymes under drought and high salinity stress, along with the differential expression of genes associated with drought and salt stress responses, enhancing rice tolerance to drought and salt. These results collectively identify OsPP2C68 as a negative regulator in the rice ABA signaling pathway. It is responsive to drought and salt stress, and involved in regulating stomatal movement. |
| format | Article |
| id | doaj-art-cc0f1250fae4470295496517f5ce3eef |
| institution | OA Journals |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Nature Portfolio |
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| series | Scientific Reports |
| spelling | doaj-art-cc0f1250fae4470295496517f5ce3eef2025-08-20T02:16:34ZengNature PortfolioScientific Reports2045-23222025-02-0115111610.1038/s41598-025-91226-2The abscisic acid signaling negative regulator OsPP2C68 confers drought and salinity tolerance to riceBeilei Wang0Yuping Luo1Binxia Zhong2Han Xu3Fengping Wang4Wen Li5Meiqi Lin6Juan Chen7Liangbi Chen8Manzhong Liang9Xiaojun Dai10Hunan Province Key Laboratory of Crop Sterile Germplasm Resource Innovation and Application, College of Life Science, Hunan Normal UniversityHunan Province Key Laboratory of Crop Sterile Germplasm Resource Innovation and Application, College of Life Science, Hunan Normal UniversityHunan Province Key Laboratory of Crop Sterile Germplasm Resource Innovation and Application, College of Life Science, Hunan Normal UniversityHunan Province Key Laboratory of Crop Sterile Germplasm Resource Innovation and Application, College of Life Science, Hunan Normal UniversityHunan Province Key Laboratory of Crop Sterile Germplasm Resource Innovation and Application, College of Life Science, Hunan Normal UniversityHunan Province Key Laboratory of Crop Sterile Germplasm Resource Innovation and Application, College of Life Science, Hunan Normal UniversityHunan Province Key Laboratory of Crop Sterile Germplasm Resource Innovation and Application, College of Life Science, Hunan Normal UniversityHunan Province Key Laboratory of Crop Sterile Germplasm Resource Innovation and Application, College of Life Science, Hunan Normal UniversityHunan Province Key Laboratory of Crop Sterile Germplasm Resource Innovation and Application, College of Life Science, Hunan Normal UniversityHunan Province Key Laboratory of Crop Sterile Germplasm Resource Innovation and Application, College of Life Science, Hunan Normal UniversityHunan Province Key Laboratory of Crop Sterile Germplasm Resource Innovation and Application, College of Life Science, Hunan Normal UniversityAbstract Clade A type 2 C protein phosphatases (PP2CAs) are core regulatory factors in the plant abscisic acid (ABA) signaling pathway. They play crucial roles in response to abiotic stress. However, a comprehensive understanding of the functions of individual members of rice PP2CA gene families remains limited. This study investigates the role of OsPP2C68 in response to abiotic stress. Our findings indicated that the OsPP2C68 is highly expressed in the embryo and endosperm, and is subcellular localized in the nucleus. OsPP2C68 knockout mutants reduced seed germination and root and stem lengths under ABA treatment. The mutants also exhibited higher stomatal closure rates, indicating increased sensitivity to ABA. In addition, the OsPP2C68 knockout mutants exhibited altered synthesis of osmolytes and antioxidant enzymes under drought and high salinity stress, along with the differential expression of genes associated with drought and salt stress responses, enhancing rice tolerance to drought and salt. These results collectively identify OsPP2C68 as a negative regulator in the rice ABA signaling pathway. It is responsive to drought and salt stress, and involved in regulating stomatal movement.https://doi.org/10.1038/s41598-025-91226-2 |
| spellingShingle | Beilei Wang Yuping Luo Binxia Zhong Han Xu Fengping Wang Wen Li Meiqi Lin Juan Chen Liangbi Chen Manzhong Liang Xiaojun Dai The abscisic acid signaling negative regulator OsPP2C68 confers drought and salinity tolerance to rice Scientific Reports |
| title | The abscisic acid signaling negative regulator OsPP2C68 confers drought and salinity tolerance to rice |
| title_full | The abscisic acid signaling negative regulator OsPP2C68 confers drought and salinity tolerance to rice |
| title_fullStr | The abscisic acid signaling negative regulator OsPP2C68 confers drought and salinity tolerance to rice |
| title_full_unstemmed | The abscisic acid signaling negative regulator OsPP2C68 confers drought and salinity tolerance to rice |
| title_short | The abscisic acid signaling negative regulator OsPP2C68 confers drought and salinity tolerance to rice |
| title_sort | abscisic acid signaling negative regulator ospp2c68 confers drought and salinity tolerance to rice |
| url | https://doi.org/10.1038/s41598-025-91226-2 |
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