Metabolomic Profiling of <i>BPH14/BPH15</i> Pyramiding Rice and Its Implications for Brown Planthopper Resistance
Rice, a vital crop, faces significant threats from the brown planthopper (BPH), which impacts plant growth and yield. Pyramiding the BPH resistance genes <i>BPH14</i> and <i>BPH15</i> provides rice crops with reliable and lasting protection against BPH. Nonetheless, current r...
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2025-06-01
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| author | Liang Hu Dabing Yang Hongbo Wang Xueshu Du Yan Wu Liang Lv Tongmin Mou Aiqing You Jinbo Li |
| author_facet | Liang Hu Dabing Yang Hongbo Wang Xueshu Du Yan Wu Liang Lv Tongmin Mou Aiqing You Jinbo Li |
| author_sort | Liang Hu |
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| description | Rice, a vital crop, faces significant threats from the brown planthopper (BPH), which impacts plant growth and yield. Pyramiding the BPH resistance genes <i>BPH14</i> and <i>BPH15</i> provides rice crops with reliable and lasting protection against BPH. Nonetheless, current research lacks clarity on the molecular processes responsible for <i>BPH14/BPH15</i>-mediated resistance to BPH. In this study, utilizing high-throughput metabolomics and integrating transcriptomic data, we investigated the metabolic adaptations of the <i>BPH14/BPH15</i> pyramiding line (B1415) and its recurrent parent (RP) during early and late infestation stages. The analysis identified 1007 metabolites, mainly consisting of lipids and lipid-like molecules, together with phenylpropanoid and polyketide classes. Differentially accumulated metabolites (DAMs) displayed different patterns in B1415 and RP, particularly in flavonoid and phenylpropanoid biosynthesis pathways, which were more pronounced in the resistant B1415. Furthermore, ferulic acid (FA) was found to negatively regulate BPH resistance. These findings elucidate critical metabolic pathways involved in rice defense mechanisms and underscore the potential of B1415’s enhanced metabolic responses in conferring durable resistance against BPH. |
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| institution | Kabale University |
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| language | English |
| publishDate | 2025-06-01 |
| publisher | MDPI AG |
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| series | Agronomy |
| spelling | doaj-art-cc02f1d46ebe49d08910803aa60caff42025-08-20T03:26:20ZengMDPI AGAgronomy2073-43952025-06-01156142810.3390/agronomy15061428Metabolomic Profiling of <i>BPH14/BPH15</i> Pyramiding Rice and Its Implications for Brown Planthopper ResistanceLiang Hu0Dabing Yang1Hongbo Wang2Xueshu Du3Yan Wu4Liang Lv5Tongmin Mou6Aiqing You7Jinbo Li8Key Laboratory of Crop Molecular Breeding, Ministry of Agriculture and Rural Affairs, Hubei Key Laboratory of Food Crop Germplasm and Genetic Improvement, Institute of Food Crops, Hubei Academy of Agricultural Sciences, Wuhan 430064, ChinaKey Laboratory of Crop Molecular Breeding, Ministry of Agriculture and Rural Affairs, Hubei Key Laboratory of Food Crop Germplasm and Genetic Improvement, Institute of Food Crops, Hubei Academy of Agricultural Sciences, Wuhan 430064, ChinaKey Laboratory of Crop Molecular Breeding, Ministry of Agriculture and Rural Affairs, Hubei Key Laboratory of Food Crop Germplasm and Genetic Improvement, Institute of Food Crops, Hubei Academy of Agricultural Sciences, Wuhan 430064, ChinaKey Laboratory of Crop Molecular Breeding, Ministry of Agriculture and Rural Affairs, Hubei Key Laboratory of Food Crop Germplasm and Genetic Improvement, Institute of Food Crops, Hubei Academy of Agricultural Sciences, Wuhan 430064, ChinaKey Laboratory of Crop Molecular Breeding, Ministry of Agriculture and Rural Affairs, Hubei Key Laboratory of Food Crop Germplasm and Genetic Improvement, Institute of Food Crops, Hubei Academy of Agricultural Sciences, Wuhan 430064, ChinaKey Laboratory of Integrated Pests Management on Crops in Central China, Ministry of Agriculture and Rural Affairs, Hubei Key Laboratory of Crop Diseases, Insect Pests and Weeds Control, Institute of Plant Protection and Soil Fertilizer, Hubei Academy of Agricultural Sciences, Wuhan 430064, ChinaNational Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, ChinaKey Laboratory of Crop Molecular Breeding, Ministry of Agriculture and Rural Affairs, Hubei Key Laboratory of Food Crop Germplasm and Genetic Improvement, Institute of Food Crops, Hubei Academy of Agricultural Sciences, Wuhan 430064, ChinaKey Laboratory of Crop Molecular Breeding, Ministry of Agriculture and Rural Affairs, Hubei Key Laboratory of Food Crop Germplasm and Genetic Improvement, Institute of Food Crops, Hubei Academy of Agricultural Sciences, Wuhan 430064, ChinaRice, a vital crop, faces significant threats from the brown planthopper (BPH), which impacts plant growth and yield. Pyramiding the BPH resistance genes <i>BPH14</i> and <i>BPH15</i> provides rice crops with reliable and lasting protection against BPH. Nonetheless, current research lacks clarity on the molecular processes responsible for <i>BPH14/BPH15</i>-mediated resistance to BPH. In this study, utilizing high-throughput metabolomics and integrating transcriptomic data, we investigated the metabolic adaptations of the <i>BPH14/BPH15</i> pyramiding line (B1415) and its recurrent parent (RP) during early and late infestation stages. The analysis identified 1007 metabolites, mainly consisting of lipids and lipid-like molecules, together with phenylpropanoid and polyketide classes. Differentially accumulated metabolites (DAMs) displayed different patterns in B1415 and RP, particularly in flavonoid and phenylpropanoid biosynthesis pathways, which were more pronounced in the resistant B1415. Furthermore, ferulic acid (FA) was found to negatively regulate BPH resistance. These findings elucidate critical metabolic pathways involved in rice defense mechanisms and underscore the potential of B1415’s enhanced metabolic responses in conferring durable resistance against BPH.https://www.mdpi.com/2073-4395/15/6/1428brown planthopperricemetabolometranscriptome<i>BPH14/BPH15</i> |
| spellingShingle | Liang Hu Dabing Yang Hongbo Wang Xueshu Du Yan Wu Liang Lv Tongmin Mou Aiqing You Jinbo Li Metabolomic Profiling of <i>BPH14/BPH15</i> Pyramiding Rice and Its Implications for Brown Planthopper Resistance Agronomy brown planthopper rice metabolome transcriptome <i>BPH14/BPH15</i> |
| title | Metabolomic Profiling of <i>BPH14/BPH15</i> Pyramiding Rice and Its Implications for Brown Planthopper Resistance |
| title_full | Metabolomic Profiling of <i>BPH14/BPH15</i> Pyramiding Rice and Its Implications for Brown Planthopper Resistance |
| title_fullStr | Metabolomic Profiling of <i>BPH14/BPH15</i> Pyramiding Rice and Its Implications for Brown Planthopper Resistance |
| title_full_unstemmed | Metabolomic Profiling of <i>BPH14/BPH15</i> Pyramiding Rice and Its Implications for Brown Planthopper Resistance |
| title_short | Metabolomic Profiling of <i>BPH14/BPH15</i> Pyramiding Rice and Its Implications for Brown Planthopper Resistance |
| title_sort | metabolomic profiling of i bph14 bph15 i pyramiding rice and its implications for brown planthopper resistance |
| topic | brown planthopper rice metabolome transcriptome <i>BPH14/BPH15</i> |
| url | https://www.mdpi.com/2073-4395/15/6/1428 |
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