Methylation Marks of Blood Leukocytes of Native Hucul Mares Differentiated in Age
Horses are one of the longest-living species of farm animals. Advanced age is often associated with a decrease in body condition, dysfunction of immune system, and late-onset disorders. Due to this, the search for new solutions in the prevention and treatment of pathological conditions of the advanc...
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| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2019-01-01
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| Series: | International Journal of Genomics |
| Online Access: | http://dx.doi.org/10.1155/2019/2839614 |
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| author | T. Ząbek E. Semik-Gurgul T. Szmatoła A. Gurgul A. Fornal M. Bugno-Poniewierska |
| author_facet | T. Ząbek E. Semik-Gurgul T. Szmatoła A. Gurgul A. Fornal M. Bugno-Poniewierska |
| author_sort | T. Ząbek |
| collection | DOAJ |
| description | Horses are one of the longest-living species of farm animals. Advanced age is often associated with a decrease in body condition, dysfunction of immune system, and late-onset disorders. Due to this, the search for new solutions in the prevention and treatment of pathological conditions of the advanced age of horses is desirable. That is why the identification of aging-related changes in the horse genome is interesting in this respect. In the recent years, the research on aging includes studies of age-related epigenetic effects observed on the DNA methylation level. We applied reduced representation bisulfite sequencing (RRBS) to uncover a range of age DMR sites in genomes of blood leukocytes derived from juvenile and aged horses of native Hucul breed. Genes colocated with age-related differentially methylated regions (age DMRs) are the members of pathways involved in cellular signal transduction, immune response, neurogenesis, differentiation, development, and cancer progression. A positive correlation was found between methylation states and gene expression in particular loci from our data set. Some of described age DMR-linked genes were also reported elsewhere. Obtained results contribute to the knowledge about the molecular basis of aging of equine blood cells. |
| format | Article |
| id | doaj-art-cbffacb511f948c4bf6b41fc1c4dc1b1 |
| institution | Kabale University |
| issn | 2314-436X 2314-4378 |
| language | English |
| publishDate | 2019-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Genomics |
| spelling | doaj-art-cbffacb511f948c4bf6b41fc1c4dc1b12025-02-03T06:13:13ZengWileyInternational Journal of Genomics2314-436X2314-43782019-01-01201910.1155/2019/28396142839614Methylation Marks of Blood Leukocytes of Native Hucul Mares Differentiated in AgeT. Ząbek0E. Semik-Gurgul1T. Szmatoła2A. Gurgul3A. Fornal4M. Bugno-Poniewierska5National Research Institute of Animal Production, Balice 32-083, PolandNational Research Institute of Animal Production, Balice 32-083, PolandNational Research Institute of Animal Production, Balice 32-083, PolandNational Research Institute of Animal Production, Balice 32-083, PolandNational Research Institute of Animal Production, Balice 32-083, PolandInstitute of Veterinary Sciences, University of Agriculture in Krakow, 31-120, PolandHorses are one of the longest-living species of farm animals. Advanced age is often associated with a decrease in body condition, dysfunction of immune system, and late-onset disorders. Due to this, the search for new solutions in the prevention and treatment of pathological conditions of the advanced age of horses is desirable. That is why the identification of aging-related changes in the horse genome is interesting in this respect. In the recent years, the research on aging includes studies of age-related epigenetic effects observed on the DNA methylation level. We applied reduced representation bisulfite sequencing (RRBS) to uncover a range of age DMR sites in genomes of blood leukocytes derived from juvenile and aged horses of native Hucul breed. Genes colocated with age-related differentially methylated regions (age DMRs) are the members of pathways involved in cellular signal transduction, immune response, neurogenesis, differentiation, development, and cancer progression. A positive correlation was found between methylation states and gene expression in particular loci from our data set. Some of described age DMR-linked genes were also reported elsewhere. Obtained results contribute to the knowledge about the molecular basis of aging of equine blood cells.http://dx.doi.org/10.1155/2019/2839614 |
| spellingShingle | T. Ząbek E. Semik-Gurgul T. Szmatoła A. Gurgul A. Fornal M. Bugno-Poniewierska Methylation Marks of Blood Leukocytes of Native Hucul Mares Differentiated in Age International Journal of Genomics |
| title | Methylation Marks of Blood Leukocytes of Native Hucul Mares Differentiated in Age |
| title_full | Methylation Marks of Blood Leukocytes of Native Hucul Mares Differentiated in Age |
| title_fullStr | Methylation Marks of Blood Leukocytes of Native Hucul Mares Differentiated in Age |
| title_full_unstemmed | Methylation Marks of Blood Leukocytes of Native Hucul Mares Differentiated in Age |
| title_short | Methylation Marks of Blood Leukocytes of Native Hucul Mares Differentiated in Age |
| title_sort | methylation marks of blood leukocytes of native hucul mares differentiated in age |
| url | http://dx.doi.org/10.1155/2019/2839614 |
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