The intratumoral landscape of T cell receptor repertoire in esophageal squamous cell carcinoma
Abstract Background Esophageal squamous cell carcinoma (ESCC) is a malignant neoplasm with detrimental implications for human health. The landscape of ESCC therapy has been revolutionized by the introduction of immunotherapy, specifically involving immune checkpoint inhibitors (ICIs). A number of st...
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2024-11-01
|
| Series: | Journal of Translational Medicine |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12967-024-05825-0 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850057902374518784 |
|---|---|
| author | Meng Yang Dan He Yu Sun Yunquan Guo Yu Ma Lin Feng Meng Liu |
| author_facet | Meng Yang Dan He Yu Sun Yunquan Guo Yu Ma Lin Feng Meng Liu |
| author_sort | Meng Yang |
| collection | DOAJ |
| description | Abstract Background Esophageal squamous cell carcinoma (ESCC) is a malignant neoplasm with detrimental implications for human health. The landscape of ESCC therapy has been revolutionized by the introduction of immunotherapy, specifically involving immune checkpoint inhibitors (ICIs). A number of studies have documented the prognostic significance of T-cell receptor (TCR) repertoire and its association with many tumors. Nevertheless, the TCR repertoire landscape and its significance in ESCC still need to be explored. Methods In this study, we conducted RNA-Seq analysis to investigate the characteristics of the TCR repertoire in 90 patients. Moreover, high-throughput TCR sequencing was performed on tumor tissues from 41 patients who received immunotherapy. Additionally, a comprehensive analysis of the T-cell receptor repertoire landscape within ESCC tumors was carried out through immunohistochemical staining on all patient samples. Results We noticed a diminished diversity of TCR repertoire within the tumor compared to its adjacent normal tissue. In terms of immunotherapy responses, non-responsive patients exhibited higher TCR repertoire diversity indices and an increased frequency of common V and J genes. Additionally, elevated TCR repertoire diversity correlated with improved overall survival rates. Lastly, immunohistochemical staining results indicated a correlation between TCR repertoire diversity and the tumor immune microenvironment (TIME). Conclusions Our study primarily describes the landscape of TCR repertoires in ESCC through three aspects: differences in tumor tissues, immune response to immunotherapy, and survival prognosis of patients. These results emphasize the importance of TCR repertoire characteristics as unique and relevant biomarkers for ESCC immunotherapy. |
| format | Article |
| id | doaj-art-cbff68607cde4c7eb4022dd69fc6cb51 |
| institution | DOAJ |
| issn | 1479-5876 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Translational Medicine |
| spelling | doaj-art-cbff68607cde4c7eb4022dd69fc6cb512025-08-20T02:51:18ZengBMCJournal of Translational Medicine1479-58762024-11-0122111310.1186/s12967-024-05825-0The intratumoral landscape of T cell receptor repertoire in esophageal squamous cell carcinomaMeng Yang0Dan He1Yu Sun2Yunquan Guo3Yu Ma4Lin Feng5Meng Liu6Affiliated Tumor Hospital of Xinjiang Medical UniversityAffiliated Tumor Hospital of Xinjiang Medical UniversityState Key Laboratory of Molecular Oncology, Department of Etiology and Carcinogenesis, National Clinical Research Center for Cancer/Cancer Hospital, National Cancer Center, Chinese Academy of Medical Sciences, Peking Union Medical CollegeAffiliated Tumor Hospital of Xinjiang Medical UniversityThe Fourth People’ Hospital of Urumqi, Xinjiang Uygur Autonomous RegionState Key Laboratory of Molecular Oncology, Department of Etiology and Carcinogenesis, National Clinical Research Center for Cancer/Cancer Hospital, National Cancer Center, Chinese Academy of Medical Sciences, Peking Union Medical CollegeAffiliated Tumor Hospital of Xinjiang Medical UniversityAbstract Background Esophageal squamous cell carcinoma (ESCC) is a malignant neoplasm with detrimental implications for human health. The landscape of ESCC therapy has been revolutionized by the introduction of immunotherapy, specifically involving immune checkpoint inhibitors (ICIs). A number of studies have documented the prognostic significance of T-cell receptor (TCR) repertoire and its association with many tumors. Nevertheless, the TCR repertoire landscape and its significance in ESCC still need to be explored. Methods In this study, we conducted RNA-Seq analysis to investigate the characteristics of the TCR repertoire in 90 patients. Moreover, high-throughput TCR sequencing was performed on tumor tissues from 41 patients who received immunotherapy. Additionally, a comprehensive analysis of the T-cell receptor repertoire landscape within ESCC tumors was carried out through immunohistochemical staining on all patient samples. Results We noticed a diminished diversity of TCR repertoire within the tumor compared to its adjacent normal tissue. In terms of immunotherapy responses, non-responsive patients exhibited higher TCR repertoire diversity indices and an increased frequency of common V and J genes. Additionally, elevated TCR repertoire diversity correlated with improved overall survival rates. Lastly, immunohistochemical staining results indicated a correlation between TCR repertoire diversity and the tumor immune microenvironment (TIME). Conclusions Our study primarily describes the landscape of TCR repertoires in ESCC through three aspects: differences in tumor tissues, immune response to immunotherapy, and survival prognosis of patients. These results emphasize the importance of TCR repertoire characteristics as unique and relevant biomarkers for ESCC immunotherapy.https://doi.org/10.1186/s12967-024-05825-0T cell receptor repertoireImmunotherapy responseEsophageal squamous cell carcinomaOverall survival |
| spellingShingle | Meng Yang Dan He Yu Sun Yunquan Guo Yu Ma Lin Feng Meng Liu The intratumoral landscape of T cell receptor repertoire in esophageal squamous cell carcinoma Journal of Translational Medicine T cell receptor repertoire Immunotherapy response Esophageal squamous cell carcinoma Overall survival |
| title | The intratumoral landscape of T cell receptor repertoire in esophageal squamous cell carcinoma |
| title_full | The intratumoral landscape of T cell receptor repertoire in esophageal squamous cell carcinoma |
| title_fullStr | The intratumoral landscape of T cell receptor repertoire in esophageal squamous cell carcinoma |
| title_full_unstemmed | The intratumoral landscape of T cell receptor repertoire in esophageal squamous cell carcinoma |
| title_short | The intratumoral landscape of T cell receptor repertoire in esophageal squamous cell carcinoma |
| title_sort | intratumoral landscape of t cell receptor repertoire in esophageal squamous cell carcinoma |
| topic | T cell receptor repertoire Immunotherapy response Esophageal squamous cell carcinoma Overall survival |
| url | https://doi.org/10.1186/s12967-024-05825-0 |
| work_keys_str_mv | AT mengyang theintratumorallandscapeoftcellreceptorrepertoireinesophagealsquamouscellcarcinoma AT danhe theintratumorallandscapeoftcellreceptorrepertoireinesophagealsquamouscellcarcinoma AT yusun theintratumorallandscapeoftcellreceptorrepertoireinesophagealsquamouscellcarcinoma AT yunquanguo theintratumorallandscapeoftcellreceptorrepertoireinesophagealsquamouscellcarcinoma AT yuma theintratumorallandscapeoftcellreceptorrepertoireinesophagealsquamouscellcarcinoma AT linfeng theintratumorallandscapeoftcellreceptorrepertoireinesophagealsquamouscellcarcinoma AT mengliu theintratumorallandscapeoftcellreceptorrepertoireinesophagealsquamouscellcarcinoma AT mengyang intratumorallandscapeoftcellreceptorrepertoireinesophagealsquamouscellcarcinoma AT danhe intratumorallandscapeoftcellreceptorrepertoireinesophagealsquamouscellcarcinoma AT yusun intratumorallandscapeoftcellreceptorrepertoireinesophagealsquamouscellcarcinoma AT yunquanguo intratumorallandscapeoftcellreceptorrepertoireinesophagealsquamouscellcarcinoma AT yuma intratumorallandscapeoftcellreceptorrepertoireinesophagealsquamouscellcarcinoma AT linfeng intratumorallandscapeoftcellreceptorrepertoireinesophagealsquamouscellcarcinoma AT mengliu intratumorallandscapeoftcellreceptorrepertoireinesophagealsquamouscellcarcinoma |