Lack of biochemical signalling in GelMA leads to polarity reversion in intestinal organoids independent from mechanoreciprocity
Xenogeneic tumour origin and batch-to-batch variability of Engelbreth-Holm-Swarm sarcoma tumour cell-derived hydrogels (Matrigel, Cultrex) limit the biomedical application of organoids in tissue engineering. The gelatin-methacryloyl (GelMA) hydrogels represent a defined, tunable, and GMP-friendly al...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
SAGE Publishing
2025-06-01
|
| Series: | Journal of Tissue Engineering |
| Online Access: | https://doi.org/10.1177/20417314251345000 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849468102417317888 |
|---|---|
| author | Lenie Vanhove Thomas Van Gansbeke Bert Devriendt Ruben Van der Meeren Ruslan I. Dmitriev Irina A. Okkelman |
| author_facet | Lenie Vanhove Thomas Van Gansbeke Bert Devriendt Ruben Van der Meeren Ruslan I. Dmitriev Irina A. Okkelman |
| author_sort | Lenie Vanhove |
| collection | DOAJ |
| description | Xenogeneic tumour origin and batch-to-batch variability of Engelbreth-Holm-Swarm sarcoma tumour cell-derived hydrogels (Matrigel, Cultrex) limit the biomedical application of organoids in tissue engineering. The gelatin-methacryloyl (GelMA) hydrogels represent a defined, tunable, and GMP-friendly alternative, but they are rarely studied as alternative to Matrigel. Here, we studied effects of mechanical properties of GelMA and addition of laminin-111 on encapsulation and growth of small intestinal organoids. GelMA-embedded organoids displayed polarity reversion, resulting in apical-out and apical-basal phenotypes, independent from the matrix stiffness. Addition of laminin-111 softened hydrogels and also resulted in a partial restoration of the basal-out phenotype. Interestingly, despite the incomplete polarity restoration, GelMA-organoids still showed minor growth. GelMA stiffness and concentration influenced the transition from 3D to 2D organoid cultures. Collectively, our study confirms that tuning of GelMA mechanical properties alone cannot recapitulate the basal membrane matrix. However, controlled polarity reversion offers a tool for engineering organoids and enabling apical membrane access. |
| format | Article |
| id | doaj-art-cbff20f867164e64a37a5ca38f50d1f9 |
| institution | Kabale University |
| issn | 2041-7314 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Journal of Tissue Engineering |
| spelling | doaj-art-cbff20f867164e64a37a5ca38f50d1f92025-08-20T03:25:57ZengSAGE PublishingJournal of Tissue Engineering2041-73142025-06-011610.1177/20417314251345000Lack of biochemical signalling in GelMA leads to polarity reversion in intestinal organoids independent from mechanoreciprocityLenie Vanhove0Thomas Van Gansbeke1Bert Devriendt2Ruben Van der Meeren3Ruslan I. Dmitriev4Irina A. Okkelman5Mitochondrial Investigations Laboratory, Department of Internal Medicine and Pediatrics, Faculty of Medicine and Health Sciences, Ghent University, BelgiumRousselot BV, Darling Ingredients, Ghent, BelgiumLaboratory of Immunology, Department of Translational Physiology, Infectiology and Public Health, Faculty of Veterinary Medicine, Ghent University, Merelbeke, BelgiumRousselot BV, Darling Ingredients, Ghent, BelgiumTissue Engineering and Biomaterials Group, Department of Human Structure and Repair, Faculty of Medicine and Health Sciences, Ghent University, BelgiumTissue Engineering and Biomaterials Group, Department of Human Structure and Repair, Faculty of Medicine and Health Sciences, Ghent University, BelgiumXenogeneic tumour origin and batch-to-batch variability of Engelbreth-Holm-Swarm sarcoma tumour cell-derived hydrogels (Matrigel, Cultrex) limit the biomedical application of organoids in tissue engineering. The gelatin-methacryloyl (GelMA) hydrogels represent a defined, tunable, and GMP-friendly alternative, but they are rarely studied as alternative to Matrigel. Here, we studied effects of mechanical properties of GelMA and addition of laminin-111 on encapsulation and growth of small intestinal organoids. GelMA-embedded organoids displayed polarity reversion, resulting in apical-out and apical-basal phenotypes, independent from the matrix stiffness. Addition of laminin-111 softened hydrogels and also resulted in a partial restoration of the basal-out phenotype. Interestingly, despite the incomplete polarity restoration, GelMA-organoids still showed minor growth. GelMA stiffness and concentration influenced the transition from 3D to 2D organoid cultures. Collectively, our study confirms that tuning of GelMA mechanical properties alone cannot recapitulate the basal membrane matrix. However, controlled polarity reversion offers a tool for engineering organoids and enabling apical membrane access.https://doi.org/10.1177/20417314251345000 |
| spellingShingle | Lenie Vanhove Thomas Van Gansbeke Bert Devriendt Ruben Van der Meeren Ruslan I. Dmitriev Irina A. Okkelman Lack of biochemical signalling in GelMA leads to polarity reversion in intestinal organoids independent from mechanoreciprocity Journal of Tissue Engineering |
| title | Lack of biochemical signalling in GelMA leads to polarity reversion in intestinal organoids independent from mechanoreciprocity |
| title_full | Lack of biochemical signalling in GelMA leads to polarity reversion in intestinal organoids independent from mechanoreciprocity |
| title_fullStr | Lack of biochemical signalling in GelMA leads to polarity reversion in intestinal organoids independent from mechanoreciprocity |
| title_full_unstemmed | Lack of biochemical signalling in GelMA leads to polarity reversion in intestinal organoids independent from mechanoreciprocity |
| title_short | Lack of biochemical signalling in GelMA leads to polarity reversion in intestinal organoids independent from mechanoreciprocity |
| title_sort | lack of biochemical signalling in gelma leads to polarity reversion in intestinal organoids independent from mechanoreciprocity |
| url | https://doi.org/10.1177/20417314251345000 |
| work_keys_str_mv | AT lenievanhove lackofbiochemicalsignallingingelmaleadstopolarityreversioninintestinalorganoidsindependentfrommechanoreciprocity AT thomasvangansbeke lackofbiochemicalsignallingingelmaleadstopolarityreversioninintestinalorganoidsindependentfrommechanoreciprocity AT bertdevriendt lackofbiochemicalsignallingingelmaleadstopolarityreversioninintestinalorganoidsindependentfrommechanoreciprocity AT rubenvandermeeren lackofbiochemicalsignallingingelmaleadstopolarityreversioninintestinalorganoidsindependentfrommechanoreciprocity AT ruslanidmitriev lackofbiochemicalsignallingingelmaleadstopolarityreversioninintestinalorganoidsindependentfrommechanoreciprocity AT irinaaokkelman lackofbiochemicalsignallingingelmaleadstopolarityreversioninintestinalorganoidsindependentfrommechanoreciprocity |