Slight Pro-Inflammatory Immunomodulation Properties of Dendritic Cells by Gardnerella vaginalis: The “Invisible Man” of Bacterial Vaginosis?
Bacterial vaginosis (BV), the most common genital infection in reproductive-aged women, is associated with increased risk of sexually transmitted infections. Its etiology remains unclear, especially the role of Gardnerella (G.) vaginalis, an anaerobic bacterium characteristic of the BV-alteration of...
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| Format: | Article |
| Language: | English |
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Wiley
2016-01-01
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| Series: | Journal of Immunology Research |
| Online Access: | http://dx.doi.org/10.1155/2016/9747480 |
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| author | Thomas Bertran Patrick Brachet Marjolaine Vareille-Delarbre Julie Falenta Annie Dosgilbert Marie-Paule Vasson Christiane Forestier Arlette Tridon Bertrand Evrard |
| author_facet | Thomas Bertran Patrick Brachet Marjolaine Vareille-Delarbre Julie Falenta Annie Dosgilbert Marie-Paule Vasson Christiane Forestier Arlette Tridon Bertrand Evrard |
| author_sort | Thomas Bertran |
| collection | DOAJ |
| description | Bacterial vaginosis (BV), the most common genital infection in reproductive-aged women, is associated with increased risk of sexually transmitted infections. Its etiology remains unclear, especially the role of Gardnerella (G.) vaginalis, an anaerobic bacterium characteristic of the BV-alteration of the vaginal ecosystem. In the genital mucosa, dendritic cells (DCs) sense bacteria of the microenvironment via receptors and then orchestrate the immune response by induction of different T cell subtypes. We investigated the interactions between G. vaginalis and human monocyte-derived DCs using a wide range of bacterial concentrations (multiplicity of infection from 0.01 to 100), and the effects of this pathogen on PHA-induced lymphocyte proliferation. As observed by electron microscopy and cytometry, G. vaginalis reduced the internalization ability of DCs by forming extracellular clusters and induced neither DC maturation, nor DC secretion of cytokines, except at the highest dose with a very early DC maturation state. The same profile was observed on lymphocytes with significant increases of proliferation and cytokine secretion only at the highest bacterial concentration. Our findings indicate that G. vaginalis possesses slight immune-stimulating activities against DCs and T cells, reflecting thus a defective inflammatory response and giving rise to the atypical, non- or low-grade, inflammatory clinical disease profile. |
| format | Article |
| id | doaj-art-cbfae5d40bd54635bd2817f2e54c9ee3 |
| institution | Kabale University |
| issn | 2314-8861 2314-7156 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Immunology Research |
| spelling | doaj-art-cbfae5d40bd54635bd2817f2e54c9ee32025-02-03T01:11:25ZengWileyJournal of Immunology Research2314-88612314-71562016-01-01201610.1155/2016/97474809747480Slight Pro-Inflammatory Immunomodulation Properties of Dendritic Cells by Gardnerella vaginalis: The “Invisible Man” of Bacterial Vaginosis?Thomas Bertran0Patrick Brachet1Marjolaine Vareille-Delarbre2Julie Falenta3Annie Dosgilbert4Marie-Paule Vasson5Christiane Forestier6Arlette Tridon7Bertrand Evrard8UFR Médecine-Pharmacie, Clermont Université, Université d’Auvergne, UMR 1019 UNH, ECREIN, Laboratoire d’Immunologie, 63001 Clermont-Ferrand, FranceINRA de Theix, UMR 1019 UNH, ECREIN, 63122 Saint-Genès-Champanelle, FranceUFR Médecine-Pharmacie, Clermont Université, Université d’Auvergne, UMR 1019 UNH, ECREIN, Laboratoire d’Immunologie, 63001 Clermont-Ferrand, FranceUFR Médecine-Pharmacie, Clermont Université, Université d’Auvergne, UMR 1019 UNH, ECREIN, Laboratoire d’Immunologie, 63001 Clermont-Ferrand, FranceService d’Immunologie, CHU Clermont-Ferrand, 63001 Clermont-Ferrand, FranceUFR Pharmacie, Clermont Université, Université d’Auvergne, UMR 1019 UNH, ECREIN, Laboratoire de Biochimie, 63001 Clermont-Ferrand, FranceUFR Pharmacie, Clermont Université, Université d’Auvergne, UMR CNRS 6023 LMGE, Laboratoire de Bactériologie, 63001 Clermont-Ferrand, FranceUFR Médecine-Pharmacie, Clermont Université, Université d’Auvergne, UMR 1019 UNH, ECREIN, Laboratoire d’Immunologie, 63001 Clermont-Ferrand, FranceUFR Médecine-Pharmacie, Clermont Université, Université d’Auvergne, UMR 1019 UNH, ECREIN, Laboratoire d’Immunologie, 63001 Clermont-Ferrand, FranceBacterial vaginosis (BV), the most common genital infection in reproductive-aged women, is associated with increased risk of sexually transmitted infections. Its etiology remains unclear, especially the role of Gardnerella (G.) vaginalis, an anaerobic bacterium characteristic of the BV-alteration of the vaginal ecosystem. In the genital mucosa, dendritic cells (DCs) sense bacteria of the microenvironment via receptors and then orchestrate the immune response by induction of different T cell subtypes. We investigated the interactions between G. vaginalis and human monocyte-derived DCs using a wide range of bacterial concentrations (multiplicity of infection from 0.01 to 100), and the effects of this pathogen on PHA-induced lymphocyte proliferation. As observed by electron microscopy and cytometry, G. vaginalis reduced the internalization ability of DCs by forming extracellular clusters and induced neither DC maturation, nor DC secretion of cytokines, except at the highest dose with a very early DC maturation state. The same profile was observed on lymphocytes with significant increases of proliferation and cytokine secretion only at the highest bacterial concentration. Our findings indicate that G. vaginalis possesses slight immune-stimulating activities against DCs and T cells, reflecting thus a defective inflammatory response and giving rise to the atypical, non- or low-grade, inflammatory clinical disease profile.http://dx.doi.org/10.1155/2016/9747480 |
| spellingShingle | Thomas Bertran Patrick Brachet Marjolaine Vareille-Delarbre Julie Falenta Annie Dosgilbert Marie-Paule Vasson Christiane Forestier Arlette Tridon Bertrand Evrard Slight Pro-Inflammatory Immunomodulation Properties of Dendritic Cells by Gardnerella vaginalis: The “Invisible Man” of Bacterial Vaginosis? Journal of Immunology Research |
| title | Slight Pro-Inflammatory Immunomodulation Properties of Dendritic Cells by Gardnerella vaginalis: The “Invisible Man” of Bacterial Vaginosis? |
| title_full | Slight Pro-Inflammatory Immunomodulation Properties of Dendritic Cells by Gardnerella vaginalis: The “Invisible Man” of Bacterial Vaginosis? |
| title_fullStr | Slight Pro-Inflammatory Immunomodulation Properties of Dendritic Cells by Gardnerella vaginalis: The “Invisible Man” of Bacterial Vaginosis? |
| title_full_unstemmed | Slight Pro-Inflammatory Immunomodulation Properties of Dendritic Cells by Gardnerella vaginalis: The “Invisible Man” of Bacterial Vaginosis? |
| title_short | Slight Pro-Inflammatory Immunomodulation Properties of Dendritic Cells by Gardnerella vaginalis: The “Invisible Man” of Bacterial Vaginosis? |
| title_sort | slight pro inflammatory immunomodulation properties of dendritic cells by gardnerella vaginalis the invisible man of bacterial vaginosis |
| url | http://dx.doi.org/10.1155/2016/9747480 |
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