Stress-induced hyperglycemia and expression of glucose cell transport genes in skeletal muscle of critically ill patients: a cross-sectional study
Abstract Objective: To explore the association between diabetes and stress-induced hyperglycemia with skeletal muscle expression of key genes related to glucose transport. Methods: This is a cross-sectional study. Skeletal muscle biopsies were taken from the left vastus muscle of critically ill ad...
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Brazilian Society of Endocrinology and Metabolism
2025-04-01
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| Series: | Archives of Endocrinology and Metabolism |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S2359-39972025000201100&lng=en&tlng=en |
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| author | Priscila Bellaver Daisy Crispim Lílian Rodrigues Henrique Cristiane Bauermann Leitão Ariell Freires Schaeffer Tatiana Helena Rech Diego Paluszkiewicz Dullius |
| author_facet | Priscila Bellaver Daisy Crispim Lílian Rodrigues Henrique Cristiane Bauermann Leitão Ariell Freires Schaeffer Tatiana Helena Rech Diego Paluszkiewicz Dullius |
| author_sort | Priscila Bellaver |
| collection | DOAJ |
| description | Abstract Objective: To explore the association between diabetes and stress-induced hyperglycemia with skeletal muscle expression of key genes related to glucose transport. Methods: This is a cross-sectional study. Skeletal muscle biopsies were taken from the left vastus muscle of critically ill adult patients within 24 hours of intensive care unit admission, and the expression of the genes of interest, namely insulin receptor substrate 1 (IRS1), insulin receptor substrate 2 (IRS2), solute carrier family 2 member 1 (SLC2A1), and solute carrier family 2 member 4 (SLC2A4), was analyzed using quantitative polymerase chain reaction. The primary analysis was planned to compare the gene expression pattern between patients with and without diabetes mellitus. The secondary analyses compared the gene expression in subgroups of patients with different levels of glycemia, glycemic variability, and glycemic gap. Results: A total of 50 consecutive patients (15 with diabetes mellitus and 35 without diabetes mellitus) were included from April 2018 to September 2018. No differences in gene expression were found between patients with or without diabetes mellitus. Individuals with hyperglycemia > 200 mg/dL at intensive care unit admission exhibited a downregulation of IRS1 compared to those without (0.4 [0.1-0.8] versus 1.1 [0.3-2.2], p = 0.04). Similarly, patients with a glycemic gap ≥ 80 mg/dL exhibited a downregulation of IRS1 compared to those with a glycemic gap < 80 mg/dL (0.3 [0.1-0.7] versus 1 [0.4-2] p=0.04). There was no difference in gene expression between patients with glycemic variability higher or lower than 40 mg/dL. Conclusion: No significant changes were found in skeletal muscle expression of IRS1, IRS2, SLC2A1, and SLC2A4 in critically ill patients with or without diabetes mellitus. However, IRS1 was downregulated in patients with stress-induced hyperglycemia. |
| format | Article |
| id | doaj-art-cbf6cc4e58b542c580da3dd5600667c8 |
| institution | DOAJ |
| issn | 2359-4292 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | Brazilian Society of Endocrinology and Metabolism |
| record_format | Article |
| series | Archives of Endocrinology and Metabolism |
| spelling | doaj-art-cbf6cc4e58b542c580da3dd5600667c82025-08-20T03:17:14ZengBrazilian Society of Endocrinology and MetabolismArchives of Endocrinology and Metabolism2359-42922025-04-0169210.20945/2359-4292-2024-0417Stress-induced hyperglycemia and expression of glucose cell transport genes in skeletal muscle of critically ill patients: a cross-sectional studyPriscila Bellaverhttps://orcid.org/0000-0001-5802-9138Daisy Crispimhttps://orcid.org/0000-0001-5095-9269Lílian Rodrigues Henriquehttps://orcid.org/0000-0002-2430-0118Cristiane Bauermann Leitãohttps://orcid.org/0000-0002-2106-309XAriell Freires Schaefferhttps://orcid.org/0000-0002-1697-6468Tatiana Helena Rechhttps://orcid.org/0000-0001-9582-2821Diego Paluszkiewicz Dulliushttps://orcid.org/0000-0002-4433-1405Abstract Objective: To explore the association between diabetes and stress-induced hyperglycemia with skeletal muscle expression of key genes related to glucose transport. Methods: This is a cross-sectional study. Skeletal muscle biopsies were taken from the left vastus muscle of critically ill adult patients within 24 hours of intensive care unit admission, and the expression of the genes of interest, namely insulin receptor substrate 1 (IRS1), insulin receptor substrate 2 (IRS2), solute carrier family 2 member 1 (SLC2A1), and solute carrier family 2 member 4 (SLC2A4), was analyzed using quantitative polymerase chain reaction. The primary analysis was planned to compare the gene expression pattern between patients with and without diabetes mellitus. The secondary analyses compared the gene expression in subgroups of patients with different levels of glycemia, glycemic variability, and glycemic gap. Results: A total of 50 consecutive patients (15 with diabetes mellitus and 35 without diabetes mellitus) were included from April 2018 to September 2018. No differences in gene expression were found between patients with or without diabetes mellitus. Individuals with hyperglycemia > 200 mg/dL at intensive care unit admission exhibited a downregulation of IRS1 compared to those without (0.4 [0.1-0.8] versus 1.1 [0.3-2.2], p = 0.04). Similarly, patients with a glycemic gap ≥ 80 mg/dL exhibited a downregulation of IRS1 compared to those with a glycemic gap < 80 mg/dL (0.3 [0.1-0.7] versus 1 [0.4-2] p=0.04). There was no difference in gene expression between patients with glycemic variability higher or lower than 40 mg/dL. Conclusion: No significant changes were found in skeletal muscle expression of IRS1, IRS2, SLC2A1, and SLC2A4 in critically ill patients with or without diabetes mellitus. However, IRS1 was downregulated in patients with stress-induced hyperglycemia.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S2359-39972025000201100&lng=en&tlng=enDiabetes mellitusGlycemic controlInsulin receptor substrate proteinsGlucose transporter type 4 |
| spellingShingle | Priscila Bellaver Daisy Crispim Lílian Rodrigues Henrique Cristiane Bauermann Leitão Ariell Freires Schaeffer Tatiana Helena Rech Diego Paluszkiewicz Dullius Stress-induced hyperglycemia and expression of glucose cell transport genes in skeletal muscle of critically ill patients: a cross-sectional study Archives of Endocrinology and Metabolism Diabetes mellitus Glycemic control Insulin receptor substrate proteins Glucose transporter type 4 |
| title | Stress-induced hyperglycemia and expression of glucose cell transport genes in skeletal muscle of critically ill patients: a cross-sectional study |
| title_full | Stress-induced hyperglycemia and expression of glucose cell transport genes in skeletal muscle of critically ill patients: a cross-sectional study |
| title_fullStr | Stress-induced hyperglycemia and expression of glucose cell transport genes in skeletal muscle of critically ill patients: a cross-sectional study |
| title_full_unstemmed | Stress-induced hyperglycemia and expression of glucose cell transport genes in skeletal muscle of critically ill patients: a cross-sectional study |
| title_short | Stress-induced hyperglycemia and expression of glucose cell transport genes in skeletal muscle of critically ill patients: a cross-sectional study |
| title_sort | stress induced hyperglycemia and expression of glucose cell transport genes in skeletal muscle of critically ill patients a cross sectional study |
| topic | Diabetes mellitus Glycemic control Insulin receptor substrate proteins Glucose transporter type 4 |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S2359-39972025000201100&lng=en&tlng=en |
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