Ultrafiltration operational conditions influence in the antibacterial activity of native and thermally treated lysozyme
To obtain high quality final products, biomolecules can be exposed in production lines to stress factors such as heating, stirring and multiple surfaces contact before their commercialization. These factors can induce stability and efficacy loss. In this work, the antibacterial activity of lysozyme...
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Académie des sciences
2023-01-01
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| Series: | Comptes Rendus. Chimie |
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| Online Access: | https://comptes-rendus.academie-sciences.fr/chimie/articles/10.5802/crchim.217/ |
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| author | de Espindola, Ariane Miron, Simona M. Dutournié, Patrick Ponche, Arnaud |
| author_facet | de Espindola, Ariane Miron, Simona M. Dutournié, Patrick Ponche, Arnaud |
| author_sort | de Espindola, Ariane |
| collection | DOAJ |
| description | To obtain high quality final products, biomolecules can be exposed in production lines to stress factors such as heating, stirring and multiple surfaces contact before their commercialization. These factors can induce stability and efficacy loss. In this work, the antibacterial activity of lysozyme (LSZ), a protein used as antibiotic which acts by disrupting the bacterial cell wall, was studied after two operations: heating and ultrafiltration. Antibacterial activity after extreme stress factors is expected to decrease due to conformational changes in the protein molecule. It was observed that ultrafiltration can promote an activity decrease proportional to the applied transmembrane pressure of a non-treated lysozyme. However, when a thermally treated lysozyme (90 °C) was filtered, no additional effect was observed due to the pore passage, independently of the applied pressure. |
| format | Article |
| id | doaj-art-cbf333995a2749f0867d38c927153f90 |
| institution | Kabale University |
| issn | 1878-1543 |
| language | English |
| publishDate | 2023-01-01 |
| publisher | Académie des sciences |
| record_format | Article |
| series | Comptes Rendus. Chimie |
| spelling | doaj-art-cbf333995a2749f0867d38c927153f902025-02-07T13:37:38ZengAcadémie des sciencesComptes Rendus. Chimie1878-15432023-01-0126S114515210.5802/crchim.21710.5802/crchim.217Ultrafiltration operational conditions influence in the antibacterial activity of native and thermally treated lysozymede Espindola, Ariane0Miron, Simona M.1Dutournié, Patrick2Ponche, Arnaud3https://orcid.org/0000-0002-8993-2896Institut de Science des Matériaux de Mulhouse, Université de Haute-Alsace, CNRS IS2M UMR 7361, 3 bis rue A. Werner, 68098 Mulhouse Cedex, France; Université de Strasbourg, FranceInstitut de Science des Matériaux de Mulhouse, Université de Haute-Alsace, CNRS IS2M UMR 7361, 3 bis rue A. Werner, 68098 Mulhouse Cedex, France; Université de Strasbourg, FranceInstitut de Science des Matériaux de Mulhouse, Université de Haute-Alsace, CNRS IS2M UMR 7361, 3 bis rue A. Werner, 68098 Mulhouse Cedex, France; Université de Strasbourg, FranceInstitut de Science des Matériaux de Mulhouse, Université de Haute-Alsace, CNRS IS2M UMR 7361, 3 bis rue A. Werner, 68098 Mulhouse Cedex, France; Université de Strasbourg, FranceTo obtain high quality final products, biomolecules can be exposed in production lines to stress factors such as heating, stirring and multiple surfaces contact before their commercialization. These factors can induce stability and efficacy loss. In this work, the antibacterial activity of lysozyme (LSZ), a protein used as antibiotic which acts by disrupting the bacterial cell wall, was studied after two operations: heating and ultrafiltration. Antibacterial activity after extreme stress factors is expected to decrease due to conformational changes in the protein molecule. It was observed that ultrafiltration can promote an activity decrease proportional to the applied transmembrane pressure of a non-treated lysozyme. However, when a thermally treated lysozyme (90 °C) was filtered, no additional effect was observed due to the pore passage, independently of the applied pressure.https://comptes-rendus.academie-sciences.fr/chimie/articles/10.5802/crchim.217/UltrafiltrationLysozymeAntibacterialDenaturationProcessing |
| spellingShingle | de Espindola, Ariane Miron, Simona M. Dutournié, Patrick Ponche, Arnaud Ultrafiltration operational conditions influence in the antibacterial activity of native and thermally treated lysozyme Comptes Rendus. Chimie Ultrafiltration Lysozyme Antibacterial Denaturation Processing |
| title | Ultrafiltration operational conditions influence in the antibacterial activity of native and thermally treated lysozyme |
| title_full | Ultrafiltration operational conditions influence in the antibacterial activity of native and thermally treated lysozyme |
| title_fullStr | Ultrafiltration operational conditions influence in the antibacterial activity of native and thermally treated lysozyme |
| title_full_unstemmed | Ultrafiltration operational conditions influence in the antibacterial activity of native and thermally treated lysozyme |
| title_short | Ultrafiltration operational conditions influence in the antibacterial activity of native and thermally treated lysozyme |
| title_sort | ultrafiltration operational conditions influence in the antibacterial activity of native and thermally treated lysozyme |
| topic | Ultrafiltration Lysozyme Antibacterial Denaturation Processing |
| url | https://comptes-rendus.academie-sciences.fr/chimie/articles/10.5802/crchim.217/ |
| work_keys_str_mv | AT deespindolaariane ultrafiltrationoperationalconditionsinfluenceintheantibacterialactivityofnativeandthermallytreatedlysozyme AT mironsimonam ultrafiltrationoperationalconditionsinfluenceintheantibacterialactivityofnativeandthermallytreatedlysozyme AT dutourniepatrick ultrafiltrationoperationalconditionsinfluenceintheantibacterialactivityofnativeandthermallytreatedlysozyme AT ponchearnaud ultrafiltrationoperationalconditionsinfluenceintheantibacterialactivityofnativeandthermallytreatedlysozyme |