Plasma Concentrations of Hepcidin in Anemic Zimbabwean Infants.
<h4>Objective</h4>Anemia in infancy is a global public health problem. We evaluated the relative contributions of iron deficiency and inflammation to infant anemia.<h4>Methods</h4>We measured plasma hepcidin, ferritin, soluble transferrin receptor (sTfR), alpha-1-acid glycopr...
Saved in:
| Main Authors: | , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2015-01-01
|
| Series: | PLoS ONE |
| Online Access: | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0135227&type=printable |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850125224520974336 |
|---|---|
| author | Tatenda G Mupfudze Rebecca J Stoltzfus Sandra Rukobo Lawrence H Moulton Jean H Humphrey Andrew J Prendergast SHINE Trial Team Andrew D Jones Amee Manges Goldberg Mangwadu John A Maluccio Mduduzi N N Mbuya Robert Ntozini James M Tielsch |
| author_facet | Tatenda G Mupfudze Rebecca J Stoltzfus Sandra Rukobo Lawrence H Moulton Jean H Humphrey Andrew J Prendergast SHINE Trial Team Andrew D Jones Amee Manges Goldberg Mangwadu John A Maluccio Mduduzi N N Mbuya Robert Ntozini James M Tielsch |
| author_sort | Tatenda G Mupfudze |
| collection | DOAJ |
| description | <h4>Objective</h4>Anemia in infancy is a global public health problem. We evaluated the relative contributions of iron deficiency and inflammation to infant anemia.<h4>Methods</h4>We measured plasma hepcidin, ferritin, soluble transferrin receptor (sTfR), alpha-1-acid glycoprotein and C-reactive protein (CRP) by ELISA on archived plasma from 289 HIV-unexposed anemic or non-anemic Zimbabwean infants at ages 3 mo, 6 mo and 12 mo. Among anemic infants, we determined the proportion with iron-deficiency anemia (IDA) and anemia of inflammation (AI). We undertook regression analyses of plasma hepcidin and anemia status, adjusting for sex, age and birthweight.<h4>Results</h4>Anemic infants at 3 mo were more stunted and had higher CRP (median 0.45 vs 0.21 mg/L; P = 0.037) and hepcidin (median 14.7 vs 9.7 ng/mL; P = 0.022) than non-anemic infants, but similar levels of ferritin and sTfR; 11% infants had IDA and 15% had AI. Anemic infants at 6 mo had higher hepcidin (median 7.9 vs 4.5 ng/mL; P = 0.016) and CRP (median 2.33 vs 0.32 mg/L; P<0.001), but lower ferritin (median 13.2 vs 25.1 μg/L; P<0.001) than non-anemic infants; 56% infants had IDA and 12% had AI. Anemic infants at 12 mo had lower ferritin (median 3.2 vs 22.2 μg/L; P<0.001) and hepcidin (median 0.9 vs 1.9 ng/mL; P = 0.019), but similar CRP levels; 48% infants had IDA and 8% had AI. Comparing anemic with non-anemic infants, plasma hepcidin was 568% higher, 405% higher and 64% lower at 3 mo, 6 mo and 12 mo, respectively, after adjusting for sex and birthweight (all p<0.01). Plasma hepcidin declined significantly with age among anemic but not non-anemic infants. Girls had 61% higher hepcidin than boys, after adjusting for age, anemia and birthweight (p<0.001).<h4>Conclusion</h4>Anemia is driven partly by inflammation early in infancy, and by iron deficiency later in infancy, with plasma hepcidin concentrations reflecting the relative contribution of each. However, there is need to better characterize the drivers of hepcidin during infancy in developing countries. |
| format | Article |
| id | doaj-art-cbefa2bd5113426983b919b0534c828b |
| institution | OA Journals |
| issn | 1932-6203 |
| language | English |
| publishDate | 2015-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj-art-cbefa2bd5113426983b919b0534c828b2025-08-20T02:34:09ZengPublic Library of Science (PLoS)PLoS ONE1932-62032015-01-01108e013522710.1371/journal.pone.0135227Plasma Concentrations of Hepcidin in Anemic Zimbabwean Infants.Tatenda G MupfudzeRebecca J StoltzfusSandra RukoboLawrence H MoultonJean H HumphreyAndrew J PrendergastSHINE Trial TeamAndrew D JonesAmee MangesGoldberg MangwaduJohn A MaluccioMduduzi N N MbuyaRobert NtoziniJames M Tielsch<h4>Objective</h4>Anemia in infancy is a global public health problem. We evaluated the relative contributions of iron deficiency and inflammation to infant anemia.<h4>Methods</h4>We measured plasma hepcidin, ferritin, soluble transferrin receptor (sTfR), alpha-1-acid glycoprotein and C-reactive protein (CRP) by ELISA on archived plasma from 289 HIV-unexposed anemic or non-anemic Zimbabwean infants at ages 3 mo, 6 mo and 12 mo. Among anemic infants, we determined the proportion with iron-deficiency anemia (IDA) and anemia of inflammation (AI). We undertook regression analyses of plasma hepcidin and anemia status, adjusting for sex, age and birthweight.<h4>Results</h4>Anemic infants at 3 mo were more stunted and had higher CRP (median 0.45 vs 0.21 mg/L; P = 0.037) and hepcidin (median 14.7 vs 9.7 ng/mL; P = 0.022) than non-anemic infants, but similar levels of ferritin and sTfR; 11% infants had IDA and 15% had AI. Anemic infants at 6 mo had higher hepcidin (median 7.9 vs 4.5 ng/mL; P = 0.016) and CRP (median 2.33 vs 0.32 mg/L; P<0.001), but lower ferritin (median 13.2 vs 25.1 μg/L; P<0.001) than non-anemic infants; 56% infants had IDA and 12% had AI. Anemic infants at 12 mo had lower ferritin (median 3.2 vs 22.2 μg/L; P<0.001) and hepcidin (median 0.9 vs 1.9 ng/mL; P = 0.019), but similar CRP levels; 48% infants had IDA and 8% had AI. Comparing anemic with non-anemic infants, plasma hepcidin was 568% higher, 405% higher and 64% lower at 3 mo, 6 mo and 12 mo, respectively, after adjusting for sex and birthweight (all p<0.01). Plasma hepcidin declined significantly with age among anemic but not non-anemic infants. Girls had 61% higher hepcidin than boys, after adjusting for age, anemia and birthweight (p<0.001).<h4>Conclusion</h4>Anemia is driven partly by inflammation early in infancy, and by iron deficiency later in infancy, with plasma hepcidin concentrations reflecting the relative contribution of each. However, there is need to better characterize the drivers of hepcidin during infancy in developing countries.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0135227&type=printable |
| spellingShingle | Tatenda G Mupfudze Rebecca J Stoltzfus Sandra Rukobo Lawrence H Moulton Jean H Humphrey Andrew J Prendergast SHINE Trial Team Andrew D Jones Amee Manges Goldberg Mangwadu John A Maluccio Mduduzi N N Mbuya Robert Ntozini James M Tielsch Plasma Concentrations of Hepcidin in Anemic Zimbabwean Infants. PLoS ONE |
| title | Plasma Concentrations of Hepcidin in Anemic Zimbabwean Infants. |
| title_full | Plasma Concentrations of Hepcidin in Anemic Zimbabwean Infants. |
| title_fullStr | Plasma Concentrations of Hepcidin in Anemic Zimbabwean Infants. |
| title_full_unstemmed | Plasma Concentrations of Hepcidin in Anemic Zimbabwean Infants. |
| title_short | Plasma Concentrations of Hepcidin in Anemic Zimbabwean Infants. |
| title_sort | plasma concentrations of hepcidin in anemic zimbabwean infants |
| url | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0135227&type=printable |
| work_keys_str_mv | AT tatendagmupfudze plasmaconcentrationsofhepcidininanemiczimbabweaninfants AT rebeccajstoltzfus plasmaconcentrationsofhepcidininanemiczimbabweaninfants AT sandrarukobo plasmaconcentrationsofhepcidininanemiczimbabweaninfants AT lawrencehmoulton plasmaconcentrationsofhepcidininanemiczimbabweaninfants AT jeanhhumphrey plasmaconcentrationsofhepcidininanemiczimbabweaninfants AT andrewjprendergast plasmaconcentrationsofhepcidininanemiczimbabweaninfants AT shinetrialteam plasmaconcentrationsofhepcidininanemiczimbabweaninfants AT andrewdjones plasmaconcentrationsofhepcidininanemiczimbabweaninfants AT ameemanges plasmaconcentrationsofhepcidininanemiczimbabweaninfants AT goldbergmangwadu plasmaconcentrationsofhepcidininanemiczimbabweaninfants AT johnamaluccio plasmaconcentrationsofhepcidininanemiczimbabweaninfants AT mduduzinnmbuya plasmaconcentrationsofhepcidininanemiczimbabweaninfants AT robertntozini plasmaconcentrationsofhepcidininanemiczimbabweaninfants AT jamesmtielsch plasmaconcentrationsofhepcidininanemiczimbabweaninfants |