Functional and microbiological effects of a microencapsulated probiotic consortium on the ruminal microbiota in vivo and in vitro systems
Fistulated animals and rumen simulation systems are essential for evaluating the effects of ingredients like probiotics, proposed as sustainable alternatives to growth-promoting antibiotics in ruminant nutrition. This study assessed the impact of microencapsulated probiotics on the structure and fun...
Saved in:
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2025-12-01
|
| Series: | Animal Biotechnology |
| Subjects: | |
| Online Access: | https://www.tandfonline.com/doi/10.1080/10495398.2025.2547345 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849228826802913280 |
|---|---|
| author | Johanna Marcela Urán Velásquez Mauricio Agudelo Rendón Mariana Zapata Wilyer García Arboleda Sara Echeverri Juan Camilo Arroyave Manco Koen Venema Juan Vasquez |
| author_facet | Johanna Marcela Urán Velásquez Mauricio Agudelo Rendón Mariana Zapata Wilyer García Arboleda Sara Echeverri Juan Camilo Arroyave Manco Koen Venema Juan Vasquez |
| author_sort | Johanna Marcela Urán Velásquez |
| collection | DOAJ |
| description | Fistulated animals and rumen simulation systems are essential for evaluating the effects of ingredients like probiotics, proposed as sustainable alternatives to growth-promoting antibiotics in ruminant nutrition. This study assessed the impact of microencapsulated probiotics on the structure and functionality of an initial ruminal microbial community using in vivo (IVV) and in vitro (IVT) systems. The IVT system was inoculated with rumen fluid obtained from the cattle animal used as IVV system. Over time, both systems were analysed for short-chain fatty acid (SCFA) production, microbial composition and functionality using next-generation sequencing. Physicochemical parameters were consistent across both systems and the inoculum, with an increase in propionate concentration observed. Although the microbial composition of IVV and IVT systems was highly similar (Pearson correlation of 0.869), significant differences in B-diversity were noted (p value = 0.023). The systems also exhibited high similarity in enzymatic profiles (correlation: 0.971) and metabolic pathways (correlation: 0.938), despite differences in functional B-diversity. Both systems showed increased production of fibrolytic enzymes, enhancing feed efficiency. The use of microencapsulated probiotics induced both taxonomic and functional changes in the initial microbial community of the IVT and IVV systems, which can be linked to the zootechnical effects of using probiotics as additives in ruminal animal nutrition. |
| format | Article |
| id | doaj-art-cbe6617bca3e44839b0daaa072b761e1 |
| institution | Kabale University |
| issn | 1049-5398 1532-2378 |
| language | English |
| publishDate | 2025-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Animal Biotechnology |
| spelling | doaj-art-cbe6617bca3e44839b0daaa072b761e12025-08-22T15:59:33ZengTaylor & Francis GroupAnimal Biotechnology1049-53981532-23782025-12-0136110.1080/10495398.2025.2547345Functional and microbiological effects of a microencapsulated probiotic consortium on the ruminal microbiota in vivo and in vitro systemsJohanna Marcela Urán Velásquez0Mauricio Agudelo Rendón1Mariana Zapata2Wilyer García Arboleda3Sara Echeverri4Juan Camilo Arroyave Manco5Koen Venema6Juan Vasquez7Research Group in Nutrition and Animal Health, Bialtec S.A.S, Medellín, ColombiaResearch Group in Nutrition and Animal Health, Bialtec S.A.S, Medellín, ColombiaResearch Group in Nutrition and Animal Health, Bialtec S.A.S, Medellín, ColombiaMineral Nutrition Research Center, Research Development and Innovation Department, SOMEX S.A.S, Medellin, ColombiaMineral Nutrition Research Center, Research Development and Innovation Department, SOMEX S.A.S, Medellin, ColombiaResearch Group in Nutrition and Animal Health, Bialtec S.A.S, Medellín, ColombiaBeneficial Microbes® Consultancy, Wageningen, the NetherlandsResearch Group in Nutrition and Animal Health, Bialtec S.A.S, Medellín, ColombiaFistulated animals and rumen simulation systems are essential for evaluating the effects of ingredients like probiotics, proposed as sustainable alternatives to growth-promoting antibiotics in ruminant nutrition. This study assessed the impact of microencapsulated probiotics on the structure and functionality of an initial ruminal microbial community using in vivo (IVV) and in vitro (IVT) systems. The IVT system was inoculated with rumen fluid obtained from the cattle animal used as IVV system. Over time, both systems were analysed for short-chain fatty acid (SCFA) production, microbial composition and functionality using next-generation sequencing. Physicochemical parameters were consistent across both systems and the inoculum, with an increase in propionate concentration observed. Although the microbial composition of IVV and IVT systems was highly similar (Pearson correlation of 0.869), significant differences in B-diversity were noted (p value = 0.023). The systems also exhibited high similarity in enzymatic profiles (correlation: 0.971) and metabolic pathways (correlation: 0.938), despite differences in functional B-diversity. Both systems showed increased production of fibrolytic enzymes, enhancing feed efficiency. The use of microencapsulated probiotics induced both taxonomic and functional changes in the initial microbial community of the IVT and IVV systems, which can be linked to the zootechnical effects of using probiotics as additives in ruminal animal nutrition.https://www.tandfonline.com/doi/10.1080/10495398.2025.2547345Functional analysismicrobial communitymicroencapsulationphysicochemical parametersprobioticrumen simulation |
| spellingShingle | Johanna Marcela Urán Velásquez Mauricio Agudelo Rendón Mariana Zapata Wilyer García Arboleda Sara Echeverri Juan Camilo Arroyave Manco Koen Venema Juan Vasquez Functional and microbiological effects of a microencapsulated probiotic consortium on the ruminal microbiota in vivo and in vitro systems Animal Biotechnology Functional analysis microbial community microencapsulation physicochemical parameters probiotic rumen simulation |
| title | Functional and microbiological effects of a microencapsulated probiotic consortium on the ruminal microbiota in vivo and in vitro systems |
| title_full | Functional and microbiological effects of a microencapsulated probiotic consortium on the ruminal microbiota in vivo and in vitro systems |
| title_fullStr | Functional and microbiological effects of a microencapsulated probiotic consortium on the ruminal microbiota in vivo and in vitro systems |
| title_full_unstemmed | Functional and microbiological effects of a microencapsulated probiotic consortium on the ruminal microbiota in vivo and in vitro systems |
| title_short | Functional and microbiological effects of a microencapsulated probiotic consortium on the ruminal microbiota in vivo and in vitro systems |
| title_sort | functional and microbiological effects of a microencapsulated probiotic consortium on the ruminal microbiota in vivo and in vitro systems |
| topic | Functional analysis microbial community microencapsulation physicochemical parameters probiotic rumen simulation |
| url | https://www.tandfonline.com/doi/10.1080/10495398.2025.2547345 |
| work_keys_str_mv | AT johannamarcelauranvelasquez functionalandmicrobiologicaleffectsofamicroencapsulatedprobioticconsortiumontheruminalmicrobiotainvivoandinvitrosystems AT mauricioagudelorendon functionalandmicrobiologicaleffectsofamicroencapsulatedprobioticconsortiumontheruminalmicrobiotainvivoandinvitrosystems AT marianazapata functionalandmicrobiologicaleffectsofamicroencapsulatedprobioticconsortiumontheruminalmicrobiotainvivoandinvitrosystems AT wilyergarciaarboleda functionalandmicrobiologicaleffectsofamicroencapsulatedprobioticconsortiumontheruminalmicrobiotainvivoandinvitrosystems AT saraecheverri functionalandmicrobiologicaleffectsofamicroencapsulatedprobioticconsortiumontheruminalmicrobiotainvivoandinvitrosystems AT juancamiloarroyavemanco functionalandmicrobiologicaleffectsofamicroencapsulatedprobioticconsortiumontheruminalmicrobiotainvivoandinvitrosystems AT koenvenema functionalandmicrobiologicaleffectsofamicroencapsulatedprobioticconsortiumontheruminalmicrobiotainvivoandinvitrosystems AT juanvasquez functionalandmicrobiologicaleffectsofamicroencapsulatedprobioticconsortiumontheruminalmicrobiotainvivoandinvitrosystems |