Mesenchymal Stem Cells Loaded with p5, Derived from CDK5 Activator p35, Inhibit Calcium-Induced CDK5 Activation in Endothelial Cells
The potential use of stem cells as therapeutics in disease has gained momentum over the last few years and recently phase-I clinical trials have shown favourable results in treatment of a small cohort of acute stroke patients. Similarly, they have been used in preclinical models drug-loaded for the...
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| Format: | Article |
| Language: | English |
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Wiley
2016-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2016/2165462 |
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| _version_ | 1849683972013948928 |
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| author | Wen-Hui Fang Shant Kumar Garry McDowell David Smith Jurek Krupinski Peter Olah Raid Saleem Al-Baradie Mohammad Othman Al-Rukban Eugene Bogdan Petcu Mark Slevin |
| author_facet | Wen-Hui Fang Shant Kumar Garry McDowell David Smith Jurek Krupinski Peter Olah Raid Saleem Al-Baradie Mohammad Othman Al-Rukban Eugene Bogdan Petcu Mark Slevin |
| author_sort | Wen-Hui Fang |
| collection | DOAJ |
| description | The potential use of stem cells as therapeutics in disease has gained momentum over the last few years and recently phase-I clinical trials have shown favourable results in treatment of a small cohort of acute stroke patients. Similarly, they have been used in preclinical models drug-loaded for the effective treatment of solid tumours. Here we have characterized uptake and release of a novel p5-cyclin-dependent kinase 5 (CDK5) inhibitory peptide by mesenchymal stem cells and showed release levels capable of blocking aberrant cyclin-dependent kinase 5 (CDK5) signaling pathways, through phosphorylation of cyclin-dependent kinase 5 (CDK5) and p53. These pathways represent the major acute mechanism stimulating apoptosis after stroke and hence its modulation could benefit patient recovery. This work indicates a potential use for drug-loaded stem cells as delivery vehicles for stroke therapeutics and in addition as anticancer receptacles particularly, if a targeting and/or holding mechanism can be defined. |
| format | Article |
| id | doaj-art-cbe061aee5d345e299cb07af26eb8a4c |
| institution | DOAJ |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-cbe061aee5d345e299cb07af26eb8a4c2025-08-20T03:23:37ZengWileyStem Cells International1687-966X1687-96782016-01-01201610.1155/2016/21654622165462Mesenchymal Stem Cells Loaded with p5, Derived from CDK5 Activator p35, Inhibit Calcium-Induced CDK5 Activation in Endothelial CellsWen-Hui Fang0Shant Kumar1Garry McDowell2David Smith3Jurek Krupinski4Peter Olah5Raid Saleem Al-Baradie6Mohammad Othman Al-Rukban7Eugene Bogdan Petcu8Mark Slevin9School of Healthcare Science, Manchester Metropolitan University, Manchester, UKSchool of Healthcare Science, Manchester Metropolitan University, Manchester, UKSchool of Healthcare Science, Manchester Metropolitan University, Manchester, UKSchool of Healthcare Science, Manchester Metropolitan University, Manchester, UKDepartment of Neurology, Hospital Universitari Mútua de Terrassa, Terrassa, Barcelona, SpainUniversity of Medicine and Pharmacy, Tirgu Mures, RomaniaDepartment of Medical Laboratories, College of Applied Medical Sciences, Majmaah University, Al Majma’ah, Saudi ArabiaDepartment of Medical Laboratories, College of Applied Medical Sciences, Majmaah University, Al Majma’ah, Saudi ArabiaGriffith University School of Medicine and Queensland Eye Institute, Griffith University, Nathan, QLD, AustraliaSchool of Healthcare Science, Manchester Metropolitan University, Manchester, UKThe potential use of stem cells as therapeutics in disease has gained momentum over the last few years and recently phase-I clinical trials have shown favourable results in treatment of a small cohort of acute stroke patients. Similarly, they have been used in preclinical models drug-loaded for the effective treatment of solid tumours. Here we have characterized uptake and release of a novel p5-cyclin-dependent kinase 5 (CDK5) inhibitory peptide by mesenchymal stem cells and showed release levels capable of blocking aberrant cyclin-dependent kinase 5 (CDK5) signaling pathways, through phosphorylation of cyclin-dependent kinase 5 (CDK5) and p53. These pathways represent the major acute mechanism stimulating apoptosis after stroke and hence its modulation could benefit patient recovery. This work indicates a potential use for drug-loaded stem cells as delivery vehicles for stroke therapeutics and in addition as anticancer receptacles particularly, if a targeting and/or holding mechanism can be defined.http://dx.doi.org/10.1155/2016/2165462 |
| spellingShingle | Wen-Hui Fang Shant Kumar Garry McDowell David Smith Jurek Krupinski Peter Olah Raid Saleem Al-Baradie Mohammad Othman Al-Rukban Eugene Bogdan Petcu Mark Slevin Mesenchymal Stem Cells Loaded with p5, Derived from CDK5 Activator p35, Inhibit Calcium-Induced CDK5 Activation in Endothelial Cells Stem Cells International |
| title | Mesenchymal Stem Cells Loaded with p5, Derived from CDK5 Activator p35, Inhibit Calcium-Induced CDK5 Activation in Endothelial Cells |
| title_full | Mesenchymal Stem Cells Loaded with p5, Derived from CDK5 Activator p35, Inhibit Calcium-Induced CDK5 Activation in Endothelial Cells |
| title_fullStr | Mesenchymal Stem Cells Loaded with p5, Derived from CDK5 Activator p35, Inhibit Calcium-Induced CDK5 Activation in Endothelial Cells |
| title_full_unstemmed | Mesenchymal Stem Cells Loaded with p5, Derived from CDK5 Activator p35, Inhibit Calcium-Induced CDK5 Activation in Endothelial Cells |
| title_short | Mesenchymal Stem Cells Loaded with p5, Derived from CDK5 Activator p35, Inhibit Calcium-Induced CDK5 Activation in Endothelial Cells |
| title_sort | mesenchymal stem cells loaded with p5 derived from cdk5 activator p35 inhibit calcium induced cdk5 activation in endothelial cells |
| url | http://dx.doi.org/10.1155/2016/2165462 |
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