DIAGNOSTIC PROCEDURE FOR DETECTION OF HPAI H5N8-BULGARIAN EXPERIENCE DURING THE EPIZOOTIC WAVE IN 2016/2017
In the 4 months spanning the 2016/2017 winter (October – January), HPAI H5N8 was the predominant serotype throughout European countries. Bulgaria, an important geographical location for migratory birds crossing Europe through the two major migratory flyways for Africa, was heavily affected with HP...
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| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Trakia University
2022-06-01
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| Series: | Trakia Journal of Sciences |
| Subjects: | |
| Online Access: | http://tru.uni-sz.bg/tsj/Volume%2020,%202022,%20Numbe%202,%20Series%20Biomedical%20Sciences/7_G.Stoimenov.pdf |
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| Summary: | In the 4 months spanning the 2016/2017 winter (October – January), HPAI H5N8 was the predominant
serotype throughout European countries. Bulgaria, an important geographical location for migratory birds
crossing Europe through the two major migratory flyways for Africa, was heavily affected with HPAI
H5N8 outbreaks. The first detection of HPAI virus serotype H5N8 in Bulgaria was on the 19th of
December 2016 in the Vidin region. Subsequently many outbreaks in wild birds and domestic poultry
were reported in 15 different administrative regions. By the end of January 2017, the HPAI H5N8 strain
was detected in domestic poultry, game birds, wild waterfowl, and zoo birds. The observed and reported
symptoms were discoordination, laboured breathing, ataxia, opisthotonos, watery diarrhoea, sudden
death, high mortality, weakness, and recumbency. In wild birds data for high mortality was only available
for some species, with well-defined hyperaemia of the meninges and brain congestion with a singular
haemorrhage being mainly observed, particularly in Dalmatian pelicans. These observations showcase
the importance of rapid and accurate detection and subtyping of these HPAI viruses by research
laboratories. To tackle this issue, the National Reference laboratory for Influenza A and Newcastle disease
in Sofia adapted a modified real-time RT-PCR assay for detection of the N8 subtype in domestic and wild
birds. The aim of this study is to present a Bulgarian experience in diagnosis of HPAI H5N8 subtype
during 2016/2017 epizootic wave. |
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| ISSN: | 1313-3551 |