Heterologous Overexpression of Cytochrome P450BM3 from <i>Bacillus megaterium</i> and Its Role in Gossypol Reduction

Heterologous Overexpression of Cytochrome P450BM3 from <i>Bacillus megaterium</i> and Its Role in Gossypol Reduction

Gossypol is a polyphenolic toxic compound present in cotton plants. To determine whether the candidate cytochrome P450BM3 enzymes could reduce gossypol in vitro, functional recombinant cytochrome P450BM3 enzymes were successfully expressed in <i>E. coli</i>. Site-directed mutagenesis gen...

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Bibliographic Details
Main Authors: Wenpeng Fan, Jingjing Cui, Tongxiang Xu, Shiheng Xu, Zulibina Ainiwaer, Qiyu Luo, Caidie Wang
Format: Article
Language:English
Published: MDPI AG 2025-05-01
Series:Toxins
Subjects:
cytochrome P450BM3
heterologous overexpression
gossypol
reduction pathways
Online Access:https://www.mdpi.com/2072-6651/17/5/253
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