Features of plasminogen activator inhibitor-1 synthesis by local fat depots of different localization in cardiovascular diseases
Aim. To determine the features of expression and secretion of plasminogen activator inhibitor-1 (PAI-1) by subcutaneous, epicardial and perivascular adipocytes depending on the complexity of coronary artery disease (CAD).Material and methods. The study included 86 patients with CAD, including 35 wit...
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| Format: | Article |
| Language: | Russian |
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«FIRMA «SILICEA» LLC
2022-05-01
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| Series: | Российский кардиологический журнал |
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| Online Access: | https://russjcardiol.elpub.ru/jour/article/view/4866 |
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| author | E. V. Belik O. V. Gruzdeva Yu. A. Dyleva E. E. Bychkova A. A. Kuzmina S. V. Ivanov O. L. Barbarash |
| author_facet | E. V. Belik O. V. Gruzdeva Yu. A. Dyleva E. E. Bychkova A. A. Kuzmina S. V. Ivanov O. L. Barbarash |
| author_sort | E. V. Belik |
| collection | DOAJ |
| description | Aim. To determine the features of expression and secretion of plasminogen activator inhibitor-1 (PAI-1) by subcutaneous, epicardial and perivascular adipocytes depending on the complexity of coronary artery disease (CAD).Material and methods. The study included 86 patients with CAD, including 35 with moderate atherosclerotic lesions of the coronary arteries (CA) (SYNTAX Score ≤22), 22 with severe (SYNTAX Score of 23-31 pints), 29 with extremely severe (SYNTAX Score ≥32). As a comparison group, 52 patients with heart defects were examined. During an elective surgical intervention, adipose tissue (AT) samples were obtained for subsequent cultivation and determination of PAI-1 gene expression and PAI-1 secretion in AT supernatants of various localizations. Statistical analysis was performed using Statistica 10.0.Results. In CAD, PAI-1 production in all three types of AT and plasma PAI-1 concentration were increased compared with patients with heart defects. Epicardial AT (EAT) in CAD was characterized by the maximum levels of expression and secretion of PAI-1 relative to patients with heart defects and subcutaneous and perivascular (PVAT) fat depots. Moderate coronary involvement (adipose tissue <22) is characterized by the lowest expression and secretion of PAI-1 in all types of AT and plasma concentration of PAI-1. Direct correlations of PAI-1 expression and secretion in EAT and PVAT and plasma PAI-1 level with its secretion in EAT in patients with CAD and heart defects were revealed. A direct correlation between the plasma level of PAI-1 and its secretion in PVAT was found only in CAD.Conclusion. In CAD, there is not only an increase in the plasma PAI-1 level, but also an increase in the expression of PAI-1 gene and the secretion of PAI-1 in all three types of AT compared with patients with heart defects. PAI-1 expression and secretion in EAT, PAI-1 plasma level, and age are predictors of severe/extremely severe coronary involvement in CAD. |
| format | Article |
| id | doaj-art-cbc37a9205284153b163d79ff1473542 |
| institution | Kabale University |
| issn | 1560-4071 2618-7620 |
| language | Russian |
| publishDate | 2022-05-01 |
| publisher | «FIRMA «SILICEA» LLC |
| record_format | Article |
| series | Российский кардиологический журнал |
| spelling | doaj-art-cbc37a9205284153b163d79ff14735422025-08-20T03:43:37Zrus«FIRMA «SILICEA» LLCРоссийский кардиологический журнал1560-40712618-76202022-05-0127410.15829/1560-4071-2022-48663494Features of plasminogen activator inhibitor-1 synthesis by local fat depots of different localization in cardiovascular diseasesE. V. Belik0O. V. Gruzdeva1Yu. A. Dyleva2E. E. Bychkova3A. A. Kuzmina4S. V. Ivanov5O. L. Barbarash6Research Institute for Complex Issues of Cardiovascular DiseasesResearch Institute for Complex Issues of Cardiovascular DiseasesResearch Institute for Complex Issues of Cardiovascular DiseasesResearch Institute for Complex Issues of Cardiovascular DiseasesResearch Institute for Complex Issues of Cardiovascular DiseasesResearch Institute for Complex Issues of Cardiovascular DiseasesResearch Institute for Complex Issues of Cardiovascular DiseasesAim. To determine the features of expression and secretion of plasminogen activator inhibitor-1 (PAI-1) by subcutaneous, epicardial and perivascular adipocytes depending on the complexity of coronary artery disease (CAD).Material and methods. The study included 86 patients with CAD, including 35 with moderate atherosclerotic lesions of the coronary arteries (CA) (SYNTAX Score ≤22), 22 with severe (SYNTAX Score of 23-31 pints), 29 with extremely severe (SYNTAX Score ≥32). As a comparison group, 52 patients with heart defects were examined. During an elective surgical intervention, adipose tissue (AT) samples were obtained for subsequent cultivation and determination of PAI-1 gene expression and PAI-1 secretion in AT supernatants of various localizations. Statistical analysis was performed using Statistica 10.0.Results. In CAD, PAI-1 production in all three types of AT and plasma PAI-1 concentration were increased compared with patients with heart defects. Epicardial AT (EAT) in CAD was characterized by the maximum levels of expression and secretion of PAI-1 relative to patients with heart defects and subcutaneous and perivascular (PVAT) fat depots. Moderate coronary involvement (adipose tissue <22) is characterized by the lowest expression and secretion of PAI-1 in all types of AT and plasma concentration of PAI-1. Direct correlations of PAI-1 expression and secretion in EAT and PVAT and plasma PAI-1 level with its secretion in EAT in patients with CAD and heart defects were revealed. A direct correlation between the plasma level of PAI-1 and its secretion in PVAT was found only in CAD.Conclusion. In CAD, there is not only an increase in the plasma PAI-1 level, but also an increase in the expression of PAI-1 gene and the secretion of PAI-1 in all three types of AT compared with patients with heart defects. PAI-1 expression and secretion in EAT, PAI-1 plasma level, and age are predictors of severe/extremely severe coronary involvement in CAD.https://russjcardiol.elpub.ru/jour/article/view/4866expressionsecretionplasminogen activator inhibitor-1epicardial adipose tissueperivascular adipose tissue |
| spellingShingle | E. V. Belik O. V. Gruzdeva Yu. A. Dyleva E. E. Bychkova A. A. Kuzmina S. V. Ivanov O. L. Barbarash Features of plasminogen activator inhibitor-1 synthesis by local fat depots of different localization in cardiovascular diseases Российский кардиологический журнал expression secretion plasminogen activator inhibitor-1 epicardial adipose tissue perivascular adipose tissue |
| title | Features of plasminogen activator inhibitor-1 synthesis by local fat depots of different localization in cardiovascular diseases |
| title_full | Features of plasminogen activator inhibitor-1 synthesis by local fat depots of different localization in cardiovascular diseases |
| title_fullStr | Features of plasminogen activator inhibitor-1 synthesis by local fat depots of different localization in cardiovascular diseases |
| title_full_unstemmed | Features of plasminogen activator inhibitor-1 synthesis by local fat depots of different localization in cardiovascular diseases |
| title_short | Features of plasminogen activator inhibitor-1 synthesis by local fat depots of different localization in cardiovascular diseases |
| title_sort | features of plasminogen activator inhibitor 1 synthesis by local fat depots of different localization in cardiovascular diseases |
| topic | expression secretion plasminogen activator inhibitor-1 epicardial adipose tissue perivascular adipose tissue |
| url | https://russjcardiol.elpub.ru/jour/article/view/4866 |
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