Cytotoxic acylphloroglucinols from the aerial parts of Hypericum hirsutum L. (Hypericaceae)

A phytochemical investigation of the dichloromethane extract of the aerial parts of Hypericum hirsutum L. led to isolating the main polyprenylated acylphloroglucinols (1 and 2). The compounds were identified employing spectral methods (HRMS, 1D, and 2D NMR, IR, UV) as adsecohyperforin (1) and secohy...

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Main Authors: Zlatina Kokanova-Nedialkova, Georgi Momekov, Paraskev T. Nedialkov
Format: Article
Language:English
Published: Pensoft Publishers 2025-03-01
Series:Pharmacia
Online Access:https://pharmacia.pensoft.net/article/148154/download/pdf/
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author Zlatina Kokanova-Nedialkova
Georgi Momekov
Paraskev T. Nedialkov
author_facet Zlatina Kokanova-Nedialkova
Georgi Momekov
Paraskev T. Nedialkov
author_sort Zlatina Kokanova-Nedialkova
collection DOAJ
description A phytochemical investigation of the dichloromethane extract of the aerial parts of Hypericum hirsutum L. led to isolating the main polyprenylated acylphloroglucinols (1 and 2). The compounds were identified employing spectral methods (HRMS, 1D, and 2D NMR, IR, UV) as adsecohyperforin (1) and secohyperforin (2), respectively. The MS/MS fragmentation of both compounds and NMR spectral data of 1 are described here for the first time. These natural products were found for the second time in a plant source. The cytotoxicity of isolated compounds was established on a panel of tumor cell lines (MDA-MB, EJ, and HL-60) and was determined using MTT-based assays. Both components showed higher cytotoxicity (IC50 = 0.27–4.85 µM) than the positive control etoposide (IC50 = 1.27–8.42 µM), making them promising candidates for new chemotherapeutic drugs.
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institution Kabale University
issn 2603-557X
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series Pharmacia
spelling doaj-art-cbc26f013ce3482183674a6a00a43d612025-08-20T03:40:22ZengPensoft PublishersPharmacia2603-557X2025-03-01721710.3897/pharmacia.72.e148154148154Cytotoxic acylphloroglucinols from the aerial parts of Hypericum hirsutum L. (Hypericaceae)Zlatina Kokanova-Nedialkova0Georgi Momekov1Paraskev T. Nedialkov2Medical University of SofiaMedical University of SofiaMedical University of SofiaA phytochemical investigation of the dichloromethane extract of the aerial parts of Hypericum hirsutum L. led to isolating the main polyprenylated acylphloroglucinols (1 and 2). The compounds were identified employing spectral methods (HRMS, 1D, and 2D NMR, IR, UV) as adsecohyperforin (1) and secohyperforin (2), respectively. The MS/MS fragmentation of both compounds and NMR spectral data of 1 are described here for the first time. These natural products were found for the second time in a plant source. The cytotoxicity of isolated compounds was established on a panel of tumor cell lines (MDA-MB, EJ, and HL-60) and was determined using MTT-based assays. Both components showed higher cytotoxicity (IC50 = 0.27–4.85 µM) than the positive control etoposide (IC50 = 1.27–8.42 µM), making them promising candidates for new chemotherapeutic drugs.https://pharmacia.pensoft.net/article/148154/download/pdf/
spellingShingle Zlatina Kokanova-Nedialkova
Georgi Momekov
Paraskev T. Nedialkov
Cytotoxic acylphloroglucinols from the aerial parts of Hypericum hirsutum L. (Hypericaceae)
Pharmacia
title Cytotoxic acylphloroglucinols from the aerial parts of Hypericum hirsutum L. (Hypericaceae)
title_full Cytotoxic acylphloroglucinols from the aerial parts of Hypericum hirsutum L. (Hypericaceae)
title_fullStr Cytotoxic acylphloroglucinols from the aerial parts of Hypericum hirsutum L. (Hypericaceae)
title_full_unstemmed Cytotoxic acylphloroglucinols from the aerial parts of Hypericum hirsutum L. (Hypericaceae)
title_short Cytotoxic acylphloroglucinols from the aerial parts of Hypericum hirsutum L. (Hypericaceae)
title_sort cytotoxic acylphloroglucinols from the aerial parts of hypericum hirsutum l hypericaceae
url https://pharmacia.pensoft.net/article/148154/download/pdf/
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AT georgimomekov cytotoxicacylphloroglucinolsfromtheaerialpartsofhypericumhirsutumlhypericaceae
AT paraskevtnedialkov cytotoxicacylphloroglucinolsfromtheaerialpartsofhypericumhirsutumlhypericaceae