Antioxidant and antimicrobial activities of Dandelion root extract (Taraxacum officinale) and its cytotoxic effect on MDA-MB-231 breast cancer cells
Abstract Breast cancer (BC) is one of the leading causes of death globally, resulting in 2.26 million cases worldwide. Treatments to downregulate these tumor cells include surgery, immunotherapy, and chemotherapy. However, habituation is expressed by the tumor cells after using the latter. Different...
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Springer
2025-02-01
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| Series: | Discover Applied Sciences |
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| Online Access: | https://doi.org/10.1007/s42452-024-06419-7 |
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| author | Ridwan Olanrewaju Shittu Ismaila Ceesay Pwadubashiyi Coston Pwavodi |
| author_facet | Ridwan Olanrewaju Shittu Ismaila Ceesay Pwadubashiyi Coston Pwavodi |
| author_sort | Ridwan Olanrewaju Shittu |
| collection | DOAJ |
| description | Abstract Breast cancer (BC) is one of the leading causes of death globally, resulting in 2.26 million cases worldwide. Treatments to downregulate these tumor cells include surgery, immunotherapy, and chemotherapy. However, habituation is expressed by the tumor cells after using the latter. Different plants have been used to treat diseases ranging from bacterial infection to cancer. Natural health products confirmed to be potent and still undergoing clinical trials include the dandelion plant, also known as Taraxacum officinale. This study evaluated Dandelion root extract's (DRE) antioxidant, antimicrobial, and cytotoxic effects. The Gas Chromatography-Mass Spectrometry (GC–MS) result revealed the presence of bioactive compounds such as P-hydroxybenzoic, chlorogenic acid, vanillic acid, caffeic acid, lupeol, leutolin, apigenin, coumestrol and much more that are present in the DRE extract. Antioxidant activity using a diphenylpicryl hydrazine (DPPH) scavenging assay was employed, and the result suggested a stable radical IC50 value of 2.860 ± 0.001 mg/mL. Trypan blue exclusion assay was used to determine the cytotoxic effect on MDA-MB-231 cells. Antimicrobial activity was assessed by disc diffusion assay, and the average diameter values obtained were 25 mm for gentamicin. No antimicrobial activity was observed for the extracts at different concentrations. DRE showed no inhibitory effect against two gram-negative and gram-positive bacteria pathogens. Our study indicates that DRE has proven novel in killing breast cancer cells, has a mildly vigorous antioxidant activity to scavenge free radicals, and has no inhibitory effect against some bacteria. |
| format | Article |
| id | doaj-art-cbbe02076dc546d98aeae0fa57128566 |
| institution | OA Journals |
| issn | 3004-9261 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Springer |
| record_format | Article |
| series | Discover Applied Sciences |
| spelling | doaj-art-cbbe02076dc546d98aeae0fa571285662025-08-20T02:12:59ZengSpringerDiscover Applied Sciences3004-92612025-02-017211510.1007/s42452-024-06419-7Antioxidant and antimicrobial activities of Dandelion root extract (Taraxacum officinale) and its cytotoxic effect on MDA-MB-231 breast cancer cellsRidwan Olanrewaju Shittu0Ismaila Ceesay1Pwadubashiyi Coston Pwavodi2Faculty of Engineering, Department of Bioengineering, Cyprus International UniversityFaculty of Engineering, Department of Bioengineering, Cyprus International UniversityFaculty of Engineering, Department of Biomedical/Medical Engineering/Bioengineering, Cyprus International UniversityAbstract Breast cancer (BC) is one of the leading causes of death globally, resulting in 2.26 million cases worldwide. Treatments to downregulate these tumor cells include surgery, immunotherapy, and chemotherapy. However, habituation is expressed by the tumor cells after using the latter. Different plants have been used to treat diseases ranging from bacterial infection to cancer. Natural health products confirmed to be potent and still undergoing clinical trials include the dandelion plant, also known as Taraxacum officinale. This study evaluated Dandelion root extract's (DRE) antioxidant, antimicrobial, and cytotoxic effects. The Gas Chromatography-Mass Spectrometry (GC–MS) result revealed the presence of bioactive compounds such as P-hydroxybenzoic, chlorogenic acid, vanillic acid, caffeic acid, lupeol, leutolin, apigenin, coumestrol and much more that are present in the DRE extract. Antioxidant activity using a diphenylpicryl hydrazine (DPPH) scavenging assay was employed, and the result suggested a stable radical IC50 value of 2.860 ± 0.001 mg/mL. Trypan blue exclusion assay was used to determine the cytotoxic effect on MDA-MB-231 cells. Antimicrobial activity was assessed by disc diffusion assay, and the average diameter values obtained were 25 mm for gentamicin. No antimicrobial activity was observed for the extracts at different concentrations. DRE showed no inhibitory effect against two gram-negative and gram-positive bacteria pathogens. Our study indicates that DRE has proven novel in killing breast cancer cells, has a mildly vigorous antioxidant activity to scavenge free radicals, and has no inhibitory effect against some bacteria.https://doi.org/10.1007/s42452-024-06419-7AntimicrobialAntioxidantBreast cancerCytotoxicityDandelion root extract |
| spellingShingle | Ridwan Olanrewaju Shittu Ismaila Ceesay Pwadubashiyi Coston Pwavodi Antioxidant and antimicrobial activities of Dandelion root extract (Taraxacum officinale) and its cytotoxic effect on MDA-MB-231 breast cancer cells Discover Applied Sciences Antimicrobial Antioxidant Breast cancer Cytotoxicity Dandelion root extract |
| title | Antioxidant and antimicrobial activities of Dandelion root extract (Taraxacum officinale) and its cytotoxic effect on MDA-MB-231 breast cancer cells |
| title_full | Antioxidant and antimicrobial activities of Dandelion root extract (Taraxacum officinale) and its cytotoxic effect on MDA-MB-231 breast cancer cells |
| title_fullStr | Antioxidant and antimicrobial activities of Dandelion root extract (Taraxacum officinale) and its cytotoxic effect on MDA-MB-231 breast cancer cells |
| title_full_unstemmed | Antioxidant and antimicrobial activities of Dandelion root extract (Taraxacum officinale) and its cytotoxic effect on MDA-MB-231 breast cancer cells |
| title_short | Antioxidant and antimicrobial activities of Dandelion root extract (Taraxacum officinale) and its cytotoxic effect on MDA-MB-231 breast cancer cells |
| title_sort | antioxidant and antimicrobial activities of dandelion root extract taraxacum officinale and its cytotoxic effect on mda mb 231 breast cancer cells |
| topic | Antimicrobial Antioxidant Breast cancer Cytotoxicity Dandelion root extract |
| url | https://doi.org/10.1007/s42452-024-06419-7 |
| work_keys_str_mv | AT ridwanolanrewajushittu antioxidantandantimicrobialactivitiesofdandelionrootextracttaraxacumofficinaleanditscytotoxiceffectonmdamb231breastcancercells AT ismailaceesay antioxidantandantimicrobialactivitiesofdandelionrootextracttaraxacumofficinaleanditscytotoxiceffectonmdamb231breastcancercells AT pwadubashiyicostonpwavodi antioxidantandantimicrobialactivitiesofdandelionrootextracttaraxacumofficinaleanditscytotoxiceffectonmdamb231breastcancercells |