Pro-inflammatory role of neutrophils populations in trauma patients: monitoring neutrophil populations
BackgroundTrauma is a leading global cause of mortality, and systemic inflammatory response syndrome (SIRS) remains a significant complication, contributing to adverse outcomes. Neutrophils, as first responders to tissue injury, undergo substantial phenotypic and functional changes following trauma....
Saved in:
| Main Authors: | , , , , , , , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2025-07-01
|
| Series: | Frontiers in Immunology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fimmu.2025.1565606/full |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849416176221814784 |
|---|---|
| author | Marcela Vlková Marcela Vlková Julie Štíchová Julie Štíchová Karolína Surá Karolína Surá Karolína Dvořáková Karolína Dvořáková Vojtěch Kunčický Vojtěch Kunčický Ioanna Papatheodorou Ioanna Papatheodorou Gabriela Blažková Zuzana Tomášiková Zuzana Tomášiková Kamila Bendíčková Kamila Bendíčková Ludmila Dohnálková Alexandra Mýtniková Alexandra Mýtniková Alexandra Mýtniková Jan Žák Jan Kovařík Milan Krtička Tomáš Tomáš Vladimír Šrámek Martin Helán Martin Helán Anna Kocurková Anna Kocurková Anna Kocurková Jan Frič Jan Frič Jan Frič Marcela Hortová-Kohoutková Marcela Hortová-Kohoutková |
| author_facet | Marcela Vlková Marcela Vlková Julie Štíchová Julie Štíchová Karolína Surá Karolína Surá Karolína Dvořáková Karolína Dvořáková Vojtěch Kunčický Vojtěch Kunčický Ioanna Papatheodorou Ioanna Papatheodorou Gabriela Blažková Zuzana Tomášiková Zuzana Tomášiková Kamila Bendíčková Kamila Bendíčková Ludmila Dohnálková Alexandra Mýtniková Alexandra Mýtniková Alexandra Mýtniková Jan Žák Jan Kovařík Milan Krtička Tomáš Tomáš Vladimír Šrámek Martin Helán Martin Helán Anna Kocurková Anna Kocurková Anna Kocurková Jan Frič Jan Frič Jan Frič Marcela Hortová-Kohoutková Marcela Hortová-Kohoutková |
| author_sort | Marcela Vlková |
| collection | DOAJ |
| description | BackgroundTrauma is a leading global cause of mortality, and systemic inflammatory response syndrome (SIRS) remains a significant complication, contributing to adverse outcomes. Neutrophils, as first responders to tissue injury, undergo substantial phenotypic and functional changes following trauma. This study investigates neutrophil subpopulations defined by CD16 and CD62L expression in trauma patients, focusing on their correlation with clinical biomarkers, trauma severity, and functional properties.MethodsWe included 50 non-infectious trauma patients, categorized into SIRS and Non-SIRS groups, and 43 elective surgery patients as controls. Neutrophil subsets were analyzed at two time points (TP1 and TP2) using flow cytometry. Functional assays evaluated phagocytosis, oxidative burst, mitochondrial function, and degranulation. Correlations between neutrophil subpopulations and clinical markers, including lactate, creatine kinase, Injury Severity Score, and Trauma and Injury Severity Score, were examined.ResultsPatients with SIRS exhibited higher proportions of banded neutrophils and CD16lowCD62Llow neutrophils at TP1, alongside reduced levels of mature neutrophils. Elevated lactate and creatine kinase levels positively correlated with banded neutrophils and CD16lowCD62Llow neutrophils, while negatively correlating with mature neutrophils CD16highCD62Lhigh and hypersegmented neutrophils CD16highCD62Llow. Hypersegmented neutrophils were more prevalent in Non-SIRS patients at TP1 and in SIRS patients at TP2. Banded neutrophils showed a positive correlation with Injury Severity Score and an inverse correlation with Trauma and Injury Severity Score (TRISS), whereas hypersegmented neutrophils were negatively associated with ISS and positively correlated with TRISS. These correlations likely reflect the pro-inflammatory role of banded neutrophils and the inflammation-resolving function of hypersegmented neutrophils. CD16lowCD62Llow neutrophils displayed impaired phagocytosis, oxidative burst, and degranulation capacity, indicating functional deficiencies.ConclusionThis study highlights the dynamic changes in neutrophil subpopulations in trauma and their association with systemic inflammation and clinical severity. Increased banded neutrophils correlate with SIRS and metabolic stress, whereas hypersegmented neutrophils may contribute to resolving inflammation. CD16lowCD62Llow neutrophils exhibit functional impairments, warranting further investigation. Monitoring neutrophil subpopulations could aid in identifying trauma patients at risk for non-infectious SIRS and guide therapeutic interventions. |
| format | Article |
| id | doaj-art-cbbaf084e38b49fc8f9b62bce24ed791 |
| institution | Kabale University |
| issn | 1664-3224 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Immunology |
| spelling | doaj-art-cbbaf084e38b49fc8f9b62bce24ed7912025-08-20T03:33:15ZengFrontiers Media S.A.Frontiers in Immunology1664-32242025-07-011610.3389/fimmu.2025.15656061565606Pro-inflammatory role of neutrophils populations in trauma patients: monitoring neutrophil populationsMarcela Vlková0Marcela Vlková1Julie Štíchová2Julie Štíchová3Karolína Surá4Karolína Surá5Karolína Dvořáková6Karolína Dvořáková7Vojtěch Kunčický8Vojtěch Kunčický9Ioanna Papatheodorou10Ioanna Papatheodorou11Gabriela Blažková12Zuzana Tomášiková13Zuzana Tomášiková14Kamila Bendíčková15Kamila Bendíčková16Ludmila Dohnálková17Alexandra Mýtniková18Alexandra Mýtniková19Alexandra Mýtniková20Jan Žák21Jan Kovařík22Milan Krtička23Tomáš Tomáš24Vladimír Šrámek25Martin Helán26Martin Helán27Anna Kocurková28Anna Kocurková29Anna Kocurková30Jan Frič31Jan Frič32Jan Frič33Marcela Hortová-Kohoutková34Marcela Hortová-Kohoutková35Institute of Clinical Immunology and Allergology, Faculty of Medicine, Masaryk University, Brno, CzechiaInstitute of Clinical Immunology and Allergology, St. Anne’s University Hospital, Brno, CzechiaInstitute of Clinical Immunology and Allergology, Faculty of Medicine, Masaryk University, Brno, CzechiaInstitute of Clinical Immunology and Allergology, St. Anne’s University Hospital, Brno, CzechiaInstitute of Clinical Immunology and Allergology, Faculty of Medicine, Masaryk University, Brno, CzechiaInstitute of Clinical Immunology and Allergology, St. Anne’s University Hospital, Brno, CzechiaInstitute of Clinical Immunology and Allergology, Faculty of Medicine, Masaryk University, Brno, CzechiaInstitute of Clinical Immunology and Allergology, St. Anne’s University Hospital, Brno, CzechiaInstitute of Clinical Immunology and Allergology, Faculty of Medicine, Masaryk University, Brno, CzechiaInstitute of Clinical Immunology and Allergology, St. Anne’s University Hospital, Brno, CzechiaInternational Clinical Research Center, St. Anne’s University Hospital, Brno, CzechiaDepartment of Biology, Faculty of Medicine, Masaryk University, Brno, CzechiaInternational Clinical Research Center, St. Anne’s University Hospital, Brno, CzechiaInternational Clinical Research Center, St. Anne’s University Hospital, Brno, CzechiaAnimal Physiology, Immunology and Developmental Biology, Faculty of Science, Masaryk University, Brno, CzechiaInternational Clinical Research Center, St. Anne’s University Hospital, Brno, CzechiaInternational Clinical Research Center, Faculty of Medicine, Masaryk University, Brno, CzechiaDepartment of Anesthesia and Intensive Care, St. Anne’s University Hospital, Brno, CzechiaInstitute of Clinical Immunology and Allergology, Faculty of Medicine, Masaryk University, Brno, CzechiaInstitute of Clinical Immunology and Allergology, St. Anne’s University Hospital, Brno, CzechiaDepartment of Anesthesia and Intensive Care, St. Anne’s University Hospital, Brno, CzechiaFirst Department of Chirurgic Surgery, St. Anne’s University Hospital and Faculty of Medicine, Masaryk University, Brno, CzechiaDepartment of Trauma Surgery, University Hospital Brno, Brno, CzechiaDepartment of Trauma Surgery, University Hospital Brno, Brno, Czechia0First Department of Orthopaedic Surgery, St. Anne’s University Hospital and Faculty of Medicine, Masaryk University, Brno, CzechiaDepartment of Anesthesia and Intensive Care, St. Anne’s University Hospital, Brno, CzechiaInternational Clinical Research Center, St. Anne’s University Hospital, Brno, CzechiaDepartment of Anesthesia and Intensive Care, St. Anne’s University Hospital, Brno, CzechiaInstitute of Clinical Immunology and Allergology, Faculty of Medicine, Masaryk University, Brno, CzechiaInstitute of Clinical Immunology and Allergology, St. Anne’s University Hospital, Brno, Czechia1Department of Biophysics of Immune System, Institute of Biophysics of the Czech Academy of Sciences, Brno, CzechiaInternational Clinical Research Center, St. Anne’s University Hospital, Brno, CzechiaInternational Clinical Research Center, Faculty of Medicine, Masaryk University, Brno, Czechia2Department of Modern Immunotherapy, Institute of Hematology and Blood Transfusion, Prague, CzechiaInternational Clinical Research Center, St. Anne’s University Hospital, Brno, CzechiaInternational Clinical Research Center, Faculty of Medicine, Masaryk University, Brno, CzechiaBackgroundTrauma is a leading global cause of mortality, and systemic inflammatory response syndrome (SIRS) remains a significant complication, contributing to adverse outcomes. Neutrophils, as first responders to tissue injury, undergo substantial phenotypic and functional changes following trauma. This study investigates neutrophil subpopulations defined by CD16 and CD62L expression in trauma patients, focusing on their correlation with clinical biomarkers, trauma severity, and functional properties.MethodsWe included 50 non-infectious trauma patients, categorized into SIRS and Non-SIRS groups, and 43 elective surgery patients as controls. Neutrophil subsets were analyzed at two time points (TP1 and TP2) using flow cytometry. Functional assays evaluated phagocytosis, oxidative burst, mitochondrial function, and degranulation. Correlations between neutrophil subpopulations and clinical markers, including lactate, creatine kinase, Injury Severity Score, and Trauma and Injury Severity Score, were examined.ResultsPatients with SIRS exhibited higher proportions of banded neutrophils and CD16lowCD62Llow neutrophils at TP1, alongside reduced levels of mature neutrophils. Elevated lactate and creatine kinase levels positively correlated with banded neutrophils and CD16lowCD62Llow neutrophils, while negatively correlating with mature neutrophils CD16highCD62Lhigh and hypersegmented neutrophils CD16highCD62Llow. Hypersegmented neutrophils were more prevalent in Non-SIRS patients at TP1 and in SIRS patients at TP2. Banded neutrophils showed a positive correlation with Injury Severity Score and an inverse correlation with Trauma and Injury Severity Score (TRISS), whereas hypersegmented neutrophils were negatively associated with ISS and positively correlated with TRISS. These correlations likely reflect the pro-inflammatory role of banded neutrophils and the inflammation-resolving function of hypersegmented neutrophils. CD16lowCD62Llow neutrophils displayed impaired phagocytosis, oxidative burst, and degranulation capacity, indicating functional deficiencies.ConclusionThis study highlights the dynamic changes in neutrophil subpopulations in trauma and their association with systemic inflammation and clinical severity. Increased banded neutrophils correlate with SIRS and metabolic stress, whereas hypersegmented neutrophils may contribute to resolving inflammation. CD16lowCD62Llow neutrophils exhibit functional impairments, warranting further investigation. Monitoring neutrophil subpopulations could aid in identifying trauma patients at risk for non-infectious SIRS and guide therapeutic interventions.https://www.frontiersin.org/articles/10.3389/fimmu.2025.1565606/fulltraumaneutrophilsSIRSlactatecreatine kinaseTRISS |
| spellingShingle | Marcela Vlková Marcela Vlková Julie Štíchová Julie Štíchová Karolína Surá Karolína Surá Karolína Dvořáková Karolína Dvořáková Vojtěch Kunčický Vojtěch Kunčický Ioanna Papatheodorou Ioanna Papatheodorou Gabriela Blažková Zuzana Tomášiková Zuzana Tomášiková Kamila Bendíčková Kamila Bendíčková Ludmila Dohnálková Alexandra Mýtniková Alexandra Mýtniková Alexandra Mýtniková Jan Žák Jan Kovařík Milan Krtička Tomáš Tomáš Vladimír Šrámek Martin Helán Martin Helán Anna Kocurková Anna Kocurková Anna Kocurková Jan Frič Jan Frič Jan Frič Marcela Hortová-Kohoutková Marcela Hortová-Kohoutková Pro-inflammatory role of neutrophils populations in trauma patients: monitoring neutrophil populations Frontiers in Immunology trauma neutrophils SIRS lactate creatine kinase TRISS |
| title | Pro-inflammatory role of neutrophils populations in trauma patients: monitoring neutrophil populations |
| title_full | Pro-inflammatory role of neutrophils populations in trauma patients: monitoring neutrophil populations |
| title_fullStr | Pro-inflammatory role of neutrophils populations in trauma patients: monitoring neutrophil populations |
| title_full_unstemmed | Pro-inflammatory role of neutrophils populations in trauma patients: monitoring neutrophil populations |
| title_short | Pro-inflammatory role of neutrophils populations in trauma patients: monitoring neutrophil populations |
| title_sort | pro inflammatory role of neutrophils populations in trauma patients monitoring neutrophil populations |
| topic | trauma neutrophils SIRS lactate creatine kinase TRISS |
| url | https://www.frontiersin.org/articles/10.3389/fimmu.2025.1565606/full |
| work_keys_str_mv | AT marcelavlkova proinflammatoryroleofneutrophilspopulationsintraumapatientsmonitoringneutrophilpopulations AT marcelavlkova proinflammatoryroleofneutrophilspopulationsintraumapatientsmonitoringneutrophilpopulations AT juliestichova proinflammatoryroleofneutrophilspopulationsintraumapatientsmonitoringneutrophilpopulations AT juliestichova proinflammatoryroleofneutrophilspopulationsintraumapatientsmonitoringneutrophilpopulations AT karolinasura proinflammatoryroleofneutrophilspopulationsintraumapatientsmonitoringneutrophilpopulations AT karolinasura proinflammatoryroleofneutrophilspopulationsintraumapatientsmonitoringneutrophilpopulations AT karolinadvorakova proinflammatoryroleofneutrophilspopulationsintraumapatientsmonitoringneutrophilpopulations AT karolinadvorakova proinflammatoryroleofneutrophilspopulationsintraumapatientsmonitoringneutrophilpopulations AT vojtechkuncicky proinflammatoryroleofneutrophilspopulationsintraumapatientsmonitoringneutrophilpopulations AT vojtechkuncicky proinflammatoryroleofneutrophilspopulationsintraumapatientsmonitoringneutrophilpopulations AT ioannapapatheodorou proinflammatoryroleofneutrophilspopulationsintraumapatientsmonitoringneutrophilpopulations AT ioannapapatheodorou proinflammatoryroleofneutrophilspopulationsintraumapatientsmonitoringneutrophilpopulations AT gabrielablazkova proinflammatoryroleofneutrophilspopulationsintraumapatientsmonitoringneutrophilpopulations AT zuzanatomasikova proinflammatoryroleofneutrophilspopulationsintraumapatientsmonitoringneutrophilpopulations AT zuzanatomasikova proinflammatoryroleofneutrophilspopulationsintraumapatientsmonitoringneutrophilpopulations AT kamilabendickova proinflammatoryroleofneutrophilspopulationsintraumapatientsmonitoringneutrophilpopulations AT kamilabendickova proinflammatoryroleofneutrophilspopulationsintraumapatientsmonitoringneutrophilpopulations AT ludmiladohnalkova proinflammatoryroleofneutrophilspopulationsintraumapatientsmonitoringneutrophilpopulations AT alexandramytnikova proinflammatoryroleofneutrophilspopulationsintraumapatientsmonitoringneutrophilpopulations AT alexandramytnikova proinflammatoryroleofneutrophilspopulationsintraumapatientsmonitoringneutrophilpopulations AT alexandramytnikova proinflammatoryroleofneutrophilspopulationsintraumapatientsmonitoringneutrophilpopulations AT janzak proinflammatoryroleofneutrophilspopulationsintraumapatientsmonitoringneutrophilpopulations AT jankovarik proinflammatoryroleofneutrophilspopulationsintraumapatientsmonitoringneutrophilpopulations AT milankrticka proinflammatoryroleofneutrophilspopulationsintraumapatientsmonitoringneutrophilpopulations AT tomastomas proinflammatoryroleofneutrophilspopulationsintraumapatientsmonitoringneutrophilpopulations AT vladimirsramek proinflammatoryroleofneutrophilspopulationsintraumapatientsmonitoringneutrophilpopulations AT martinhelan proinflammatoryroleofneutrophilspopulationsintraumapatientsmonitoringneutrophilpopulations AT martinhelan proinflammatoryroleofneutrophilspopulationsintraumapatientsmonitoringneutrophilpopulations AT annakocurkova proinflammatoryroleofneutrophilspopulationsintraumapatientsmonitoringneutrophilpopulations AT annakocurkova proinflammatoryroleofneutrophilspopulationsintraumapatientsmonitoringneutrophilpopulations AT annakocurkova proinflammatoryroleofneutrophilspopulationsintraumapatientsmonitoringneutrophilpopulations AT janfric proinflammatoryroleofneutrophilspopulationsintraumapatientsmonitoringneutrophilpopulations AT janfric proinflammatoryroleofneutrophilspopulationsintraumapatientsmonitoringneutrophilpopulations AT janfric proinflammatoryroleofneutrophilspopulationsintraumapatientsmonitoringneutrophilpopulations AT marcelahortovakohoutkova proinflammatoryroleofneutrophilspopulationsintraumapatientsmonitoringneutrophilpopulations AT marcelahortovakohoutkova proinflammatoryroleofneutrophilspopulationsintraumapatientsmonitoringneutrophilpopulations |