Involvement of the IL23/Th17 Pathway in the Pathogenesis of Tunisian Pemphigus Foliaceus
Pemphigus foliaceus (PF) is a rare autoimmune skin disease caused by anti-Dsg1 pathogenic autoantibodies. It is considered as a Th2-mediated disease. Likewise, Th17 cells were recently described in the pathogenesis of the disease but their role is still unclear. We aimed to unravel the eventual impl...
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| Format: | Article |
| Language: | English |
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Wiley
2018-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2018/8206983 |
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| author | M. Ben Jmaa O. Abida R. Fakhfakh E. Bahloul Kh. Sellami L. Gaddour N. Elloumi M. Ben Ayed A. Masmoudi M. Dhouib M. Abdelmoula N. Mahfoudh H. Makni H. Turki H. Masmoudi |
| author_facet | M. Ben Jmaa O. Abida R. Fakhfakh E. Bahloul Kh. Sellami L. Gaddour N. Elloumi M. Ben Ayed A. Masmoudi M. Dhouib M. Abdelmoula N. Mahfoudh H. Makni H. Turki H. Masmoudi |
| author_sort | M. Ben Jmaa |
| collection | DOAJ |
| description | Pemphigus foliaceus (PF) is a rare autoimmune skin disease caused by anti-Dsg1 pathogenic autoantibodies. It is considered as a Th2-mediated disease. Likewise, Th17 cells were recently described in the pathogenesis of the disease but their role is still unclear. We aimed to unravel the eventual implication of the IL23/Th17 pathway in the development of PF. A case-control study was conducted on 115 PF patients and 201 healthy controls using PCR-RFLP and AS-PCR methods. SNPs in IL23R, RORγt, IL17A, IL17F, IL17AR, TNFa, and STAT3 genes were genotyped. mRNA expression of IL23R and RORγt was evaluated using Q-PCR. The frequency of circulating Th17 cells was analyzed by flow cytometry. Genetic associations between IL23R>rs11209026, IL17A>rs3748067, IL17F>rs763780, and TNFa>rs1800629 and the susceptibility to PF were reported. Moreover, we revealed a significant increased frequency of circulating CD4+IL17+ cells as well as higher mRNA levels of RORγt and IL23R in PBMCs of patients. However, no significant increase of RORγt and IL23R mRNA expression was observed in lesional skin biopsies. In spite of the little size of specimens, our results provide converging arguments for the contribution of the IL23/Th17 pathway in the pathogenesis of PF. |
| format | Article |
| id | doaj-art-cbb137f4d2de401ebd80a1c31046015f |
| institution | DOAJ |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2018-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-cbb137f4d2de401ebd80a1c31046015f2025-08-20T03:23:37ZengWileyMediators of Inflammation0962-93511466-18612018-01-01201810.1155/2018/82069838206983Involvement of the IL23/Th17 Pathway in the Pathogenesis of Tunisian Pemphigus FoliaceusM. Ben Jmaa0O. Abida1R. Fakhfakh2E. Bahloul3Kh. Sellami4L. Gaddour5N. Elloumi6M. Ben Ayed7A. Masmoudi8M. Dhouib9M. Abdelmoula10N. Mahfoudh11H. Makni12H. Turki13H. Masmoudi14Immunology Department, Habib Bourguiba Hospital, University of Sfax, Sfax, TunisiaImmunology Department, Habib Bourguiba Hospital, University of Sfax, Sfax, TunisiaImmunology Department, Habib Bourguiba Hospital, University of Sfax, Sfax, TunisiaDermatology Department, Hedi Chaker Hospital, University of Sfax, Sfax, TunisiaDermatology Department, Hedi Chaker Hospital, University of Sfax, Sfax, TunisiaImmunology Department, Hedi Chaker Hospital, University of Sfax, Sfax, TunisiaImmunology Department, Habib Bourguiba Hospital, University of Sfax, Sfax, TunisiaImmunology Department, Habib Bourguiba Hospital, University of Sfax, Sfax, TunisiaDermatology Department, Hedi Chaker Hospital, University of Sfax, Sfax, TunisiaMaxillofacial Surgery Department, Habib Bourguiba Hospital, Sfax, TunisiaMaxillofacial Surgery Department, Habib Bourguiba Hospital, Sfax, TunisiaImmunology Department, Hedi Chaker Hospital, University of Sfax, Sfax, TunisiaImmunology Department, Hedi Chaker Hospital, University of Sfax, Sfax, TunisiaDermatology Department, Hedi Chaker Hospital, University of Sfax, Sfax, TunisiaImmunology Department, Habib Bourguiba Hospital, University of Sfax, Sfax, TunisiaPemphigus foliaceus (PF) is a rare autoimmune skin disease caused by anti-Dsg1 pathogenic autoantibodies. It is considered as a Th2-mediated disease. Likewise, Th17 cells were recently described in the pathogenesis of the disease but their role is still unclear. We aimed to unravel the eventual implication of the IL23/Th17 pathway in the development of PF. A case-control study was conducted on 115 PF patients and 201 healthy controls using PCR-RFLP and AS-PCR methods. SNPs in IL23R, RORγt, IL17A, IL17F, IL17AR, TNFa, and STAT3 genes were genotyped. mRNA expression of IL23R and RORγt was evaluated using Q-PCR. The frequency of circulating Th17 cells was analyzed by flow cytometry. Genetic associations between IL23R>rs11209026, IL17A>rs3748067, IL17F>rs763780, and TNFa>rs1800629 and the susceptibility to PF were reported. Moreover, we revealed a significant increased frequency of circulating CD4+IL17+ cells as well as higher mRNA levels of RORγt and IL23R in PBMCs of patients. However, no significant increase of RORγt and IL23R mRNA expression was observed in lesional skin biopsies. In spite of the little size of specimens, our results provide converging arguments for the contribution of the IL23/Th17 pathway in the pathogenesis of PF.http://dx.doi.org/10.1155/2018/8206983 |
| spellingShingle | M. Ben Jmaa O. Abida R. Fakhfakh E. Bahloul Kh. Sellami L. Gaddour N. Elloumi M. Ben Ayed A. Masmoudi M. Dhouib M. Abdelmoula N. Mahfoudh H. Makni H. Turki H. Masmoudi Involvement of the IL23/Th17 Pathway in the Pathogenesis of Tunisian Pemphigus Foliaceus Mediators of Inflammation |
| title | Involvement of the IL23/Th17 Pathway in the Pathogenesis of Tunisian Pemphigus Foliaceus |
| title_full | Involvement of the IL23/Th17 Pathway in the Pathogenesis of Tunisian Pemphigus Foliaceus |
| title_fullStr | Involvement of the IL23/Th17 Pathway in the Pathogenesis of Tunisian Pemphigus Foliaceus |
| title_full_unstemmed | Involvement of the IL23/Th17 Pathway in the Pathogenesis of Tunisian Pemphigus Foliaceus |
| title_short | Involvement of the IL23/Th17 Pathway in the Pathogenesis of Tunisian Pemphigus Foliaceus |
| title_sort | involvement of the il23 th17 pathway in the pathogenesis of tunisian pemphigus foliaceus |
| url | http://dx.doi.org/10.1155/2018/8206983 |
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