Exploring anticancer, antioxidant, and antimicrobial potential of Aspergillus flavus, a fungal endophyte isolated from Dillenia indica leaf callus
Background: Endophytic fungi represent a compelling assemblage of microorganisms that inhabit plant tissues without inflicting any discernible detriment to the host organism. They foster a symbiotic association with their host plants, frequently conferring advantages such as augmented growth, enhanc...
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Elsevier
2025-02-01
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| author | Ashish Gupta Brajesh Chandra Pandey Mohd Yaseen Renu Kushwaha Madhavenda Shukla Pratima Chaudhary Partha Pratim Manna Aparna Singh Ida Tiwari Gopal Nath Nishi Kumari |
| author_facet | Ashish Gupta Brajesh Chandra Pandey Mohd Yaseen Renu Kushwaha Madhavenda Shukla Pratima Chaudhary Partha Pratim Manna Aparna Singh Ida Tiwari Gopal Nath Nishi Kumari |
| author_sort | Ashish Gupta |
| collection | DOAJ |
| description | Background: Endophytic fungi represent a compelling assemblage of microorganisms that inhabit plant tissues without inflicting any discernible detriment to the host organism. They foster a symbiotic association with their host plants, frequently conferring advantages such as augmented growth, enhanced resilience to stressors, and safeguarding against pathogens. Study design: Dillenia indica is a medicinal tree of Dilleniaceae. This study aims to isolate and identify the fungi growing as a contaminant in leaf callus. For the identification, both morphological observation and molecular methods were used. The presence of secondary metabolites in different fungal extracts were observed by FTIR and High-resolution accurate mass spectroscopy (HRAMS) methods. Different biological activities (antioxidant, antibacterial and antitumor) of fungal extracts were assessed. Methods: For callus initiation, leaf tissues of Dillenia indica were inoculated on Murashige and Skoog's medium supplemented with BAP (1mgl-1) and NAA (1mgl-1) plant growth regulators. To raise pure cultures of endophyte, fungal hyphae were isolated from the contaminated cultures and were grown on Potato Dextrose Agar medium. For molecular identification, genomic DNA (gDNA) was isolated from fungal mycelia. Internal transcribed spacers (ITS1 and ITS4) were used to amplify the conserved ITS region of the fungal gDNA. Previously deposited sequences in the Gene bank were used for the identification and making of phylogenetic tree. Antioxidant, antibacterial and anticancer potential of fungal extracts were studied. Results: The endophyte was identified as Aspergillus flavus. FTIR study showed the presence of diverse types of secondary metabolites in fungal extract. A significant presence of phenolics, flavonoids, terpenes, steroids, etc. was observed by High-resolution accurate mass spectroscopy analysis (HRAMS) of fungal extract. Endophyte extract prepared in chloroform showed both antioxidant (IC50 430.23) and antibacterial (maximum inhibition of E. coli:15 ± 0.62 mm) potential compared to other solvents. Cell viability decreased at high concentrations of endophyte extract prepared in chloroform and ethyl acetate solvents. Fungal extract prepared in ethyl acetate showed considerable cytotoxicity and growth inhibition of DL tumor cells. Conclusion: In the present study, isolated endophyte of Dillenia indica showed high occurrence of secondary metabolites. Fungal extracts showed antioxidant, antibacterial and antitumor activities. As, endophytes are remarkable source of active constituents, there is a great need to explore such endophytes. Their extensive studies are required to develop an alternative of plant less production of valuable compounds. |
| format | Article |
| id | doaj-art-cbaa08366e184af489a4504271463657 |
| institution | Kabale University |
| issn | 2405-8440 |
| language | English |
| publishDate | 2025-02-01 |
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| spelling | doaj-art-cbaa08366e184af489a45042714636572025-01-29T05:01:31ZengElsevierHeliyon2405-84402025-02-01113e42142Exploring anticancer, antioxidant, and antimicrobial potential of Aspergillus flavus, a fungal endophyte isolated from Dillenia indica leaf callusAshish Gupta0Brajesh Chandra Pandey1Mohd Yaseen2Renu Kushwaha3Madhavenda Shukla4Pratima Chaudhary5Partha Pratim Manna6Aparna Singh7Ida Tiwari8Gopal Nath9Nishi Kumari10Department of Botany, Mahila Mahavidyalaya, Banaras Hindu University, Varanasi, IndiaDepartment of Botany, Mahila Mahavidyalaya, Banaras Hindu University, Varanasi, IndiaDepartment of Botany, Mahila Mahavidyalaya, Banaras Hindu University, Varanasi, IndiaDepartment of Botany, Mahila Mahavidyalaya, Banaras Hindu University, Varanasi, IndiaDepartment of Botany, Mahila Mahavidyalaya, Banaras Hindu University, Varanasi, IndiaDepartment of Zoology, Institute of Sciences, Banaras Hindu University, Varanasi, IndiaDepartment of Zoology, Institute of Sciences, Banaras Hindu University, Varanasi, IndiaDepartment of Botany, Mahila Mahavidyalaya, Banaras Hindu University, Varanasi, IndiaDepartment of Chemistry, Institute of Sciences, Banaras Hindu University, Varanasi, IndiaDepartment of Microbiology, Institute of Medical Sciences, Banaras Hindu University, Varanasi, IndiaDepartment of Botany, Mahila Mahavidyalaya, Banaras Hindu University, Varanasi, India; Corresponding author. Department of Botany Mahila Mahavidyalaya, Banaras Hindu University Varanasi-221005 UP, India.Background: Endophytic fungi represent a compelling assemblage of microorganisms that inhabit plant tissues without inflicting any discernible detriment to the host organism. They foster a symbiotic association with their host plants, frequently conferring advantages such as augmented growth, enhanced resilience to stressors, and safeguarding against pathogens. Study design: Dillenia indica is a medicinal tree of Dilleniaceae. This study aims to isolate and identify the fungi growing as a contaminant in leaf callus. For the identification, both morphological observation and molecular methods were used. The presence of secondary metabolites in different fungal extracts were observed by FTIR and High-resolution accurate mass spectroscopy (HRAMS) methods. Different biological activities (antioxidant, antibacterial and antitumor) of fungal extracts were assessed. Methods: For callus initiation, leaf tissues of Dillenia indica were inoculated on Murashige and Skoog's medium supplemented with BAP (1mgl-1) and NAA (1mgl-1) plant growth regulators. To raise pure cultures of endophyte, fungal hyphae were isolated from the contaminated cultures and were grown on Potato Dextrose Agar medium. For molecular identification, genomic DNA (gDNA) was isolated from fungal mycelia. Internal transcribed spacers (ITS1 and ITS4) were used to amplify the conserved ITS region of the fungal gDNA. Previously deposited sequences in the Gene bank were used for the identification and making of phylogenetic tree. Antioxidant, antibacterial and anticancer potential of fungal extracts were studied. Results: The endophyte was identified as Aspergillus flavus. FTIR study showed the presence of diverse types of secondary metabolites in fungal extract. A significant presence of phenolics, flavonoids, terpenes, steroids, etc. was observed by High-resolution accurate mass spectroscopy analysis (HRAMS) of fungal extract. Endophyte extract prepared in chloroform showed both antioxidant (IC50 430.23) and antibacterial (maximum inhibition of E. coli:15 ± 0.62 mm) potential compared to other solvents. Cell viability decreased at high concentrations of endophyte extract prepared in chloroform and ethyl acetate solvents. Fungal extract prepared in ethyl acetate showed considerable cytotoxicity and growth inhibition of DL tumor cells. Conclusion: In the present study, isolated endophyte of Dillenia indica showed high occurrence of secondary metabolites. Fungal extracts showed antioxidant, antibacterial and antitumor activities. As, endophytes are remarkable source of active constituents, there is a great need to explore such endophytes. Their extensive studies are required to develop an alternative of plant less production of valuable compounds.http://www.sciencedirect.com/science/article/pii/S2405844025005225AnticancerAspergillus flavusCallusDillenia indica, EndophyteSecondary metabolites |
| spellingShingle | Ashish Gupta Brajesh Chandra Pandey Mohd Yaseen Renu Kushwaha Madhavenda Shukla Pratima Chaudhary Partha Pratim Manna Aparna Singh Ida Tiwari Gopal Nath Nishi Kumari Exploring anticancer, antioxidant, and antimicrobial potential of Aspergillus flavus, a fungal endophyte isolated from Dillenia indica leaf callus Heliyon Anticancer Aspergillus flavus Callus Dillenia indica, Endophyte Secondary metabolites |
| title | Exploring anticancer, antioxidant, and antimicrobial potential of Aspergillus flavus, a fungal endophyte isolated from Dillenia indica leaf callus |
| title_full | Exploring anticancer, antioxidant, and antimicrobial potential of Aspergillus flavus, a fungal endophyte isolated from Dillenia indica leaf callus |
| title_fullStr | Exploring anticancer, antioxidant, and antimicrobial potential of Aspergillus flavus, a fungal endophyte isolated from Dillenia indica leaf callus |
| title_full_unstemmed | Exploring anticancer, antioxidant, and antimicrobial potential of Aspergillus flavus, a fungal endophyte isolated from Dillenia indica leaf callus |
| title_short | Exploring anticancer, antioxidant, and antimicrobial potential of Aspergillus flavus, a fungal endophyte isolated from Dillenia indica leaf callus |
| title_sort | exploring anticancer antioxidant and antimicrobial potential of aspergillus flavus a fungal endophyte isolated from dillenia indica leaf callus |
| topic | Anticancer Aspergillus flavus Callus Dillenia indica, Endophyte Secondary metabolites |
| url | http://www.sciencedirect.com/science/article/pii/S2405844025005225 |
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