Adipose tissue and adipose-derived stromal cells can reduce skin contraction in an in vitro tissue engineered full thickness skin model
Skin contracts during wound healing to facilitate wound closure. In some patients, skin contraction can lead to the formation of skin contractures that limit movement, impair function, and significantly impact well-being. Current treatment options for skin contractures are burdensome for patients, a...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2025-12-01
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| Series: | Adipocyte |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/21623945.2025.2473367 |
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| author | Victoria L. Workman Anna‑Victoria Giblin Nicola H. Green Sheila MacNeil Vanessa Hearnden |
| author_facet | Victoria L. Workman Anna‑Victoria Giblin Nicola H. Green Sheila MacNeil Vanessa Hearnden |
| author_sort | Victoria L. Workman |
| collection | DOAJ |
| description | Skin contracts during wound healing to facilitate wound closure. In some patients, skin contraction can lead to the formation of skin contractures that limit movement, impair function, and significantly impact well-being. Current treatment options for skin contractures are burdensome for patients, and there is a high risk of recurrence. Autologous fat grafting can improve the structure and function of scarred skin; however, relatively little is known about the effect of fat on skin contraction. In this study, an in vitro tissue-engineered model of human skin was used to test the effects of adipose tissue and adipose-derived stromal cells on skin contraction. Untreated tissue-engineered skin contracted to approximately 60% of the original area over 14 days in culture. The addition of adipose tissue reduced this contraction by 50%. Adipose tissue, which was emulsified or concentrated and high doses of adipose-derived stromal cells (ADSC) were able to inhibit contraction to a similar degree; however, lower doses of ADSC did not show the same effect. In conclusion, the subcutaneous application of adipose tissue has the potential to inhibit skin contraction. This study provides in vitro evidence to support the use of autologous fat grafting to prevent skin contraction in patients most at risk. |
| format | Article |
| id | doaj-art-cba73a4781fc46b7a698220f756ebdd1 |
| institution | DOAJ |
| issn | 2162-3945 2162-397X |
| language | English |
| publishDate | 2025-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Adipocyte |
| spelling | doaj-art-cba73a4781fc46b7a698220f756ebdd12025-08-20T03:02:38ZengTaylor & Francis GroupAdipocyte2162-39452162-397X2025-12-0114110.1080/21623945.2025.2473367Adipose tissue and adipose-derived stromal cells can reduce skin contraction in an in vitro tissue engineered full thickness skin modelVictoria L. Workman0Anna‑Victoria Giblin1Nicola H. Green2Sheila MacNeil3Vanessa Hearnden4Department of Materials Science and Engineering, University of Sheffield, Sheffield, UKDepartment of Plastic Surgery, Sheffield Teaching Hospitals, NHS Foundation Trust, Sheffield, UKDepartment of Materials Science and Engineering, University of Sheffield, Sheffield, UKDepartment of Materials Science and Engineering, University of Sheffield, Sheffield, UKDepartment of Materials Science and Engineering, University of Sheffield, Sheffield, UKSkin contracts during wound healing to facilitate wound closure. In some patients, skin contraction can lead to the formation of skin contractures that limit movement, impair function, and significantly impact well-being. Current treatment options for skin contractures are burdensome for patients, and there is a high risk of recurrence. Autologous fat grafting can improve the structure and function of scarred skin; however, relatively little is known about the effect of fat on skin contraction. In this study, an in vitro tissue-engineered model of human skin was used to test the effects of adipose tissue and adipose-derived stromal cells on skin contraction. Untreated tissue-engineered skin contracted to approximately 60% of the original area over 14 days in culture. The addition of adipose tissue reduced this contraction by 50%. Adipose tissue, which was emulsified or concentrated and high doses of adipose-derived stromal cells (ADSC) were able to inhibit contraction to a similar degree; however, lower doses of ADSC did not show the same effect. In conclusion, the subcutaneous application of adipose tissue has the potential to inhibit skin contraction. This study provides in vitro evidence to support the use of autologous fat grafting to prevent skin contraction in patients most at risk.https://www.tandfonline.com/doi/10.1080/21623945.2025.2473367Skin contractionadipose tissuefat graftingadipose-derived stromal cellscontracture |
| spellingShingle | Victoria L. Workman Anna‑Victoria Giblin Nicola H. Green Sheila MacNeil Vanessa Hearnden Adipose tissue and adipose-derived stromal cells can reduce skin contraction in an in vitro tissue engineered full thickness skin model Adipocyte Skin contraction adipose tissue fat grafting adipose-derived stromal cells contracture |
| title | Adipose tissue and adipose-derived stromal cells can reduce skin contraction in an in vitro tissue engineered full thickness skin model |
| title_full | Adipose tissue and adipose-derived stromal cells can reduce skin contraction in an in vitro tissue engineered full thickness skin model |
| title_fullStr | Adipose tissue and adipose-derived stromal cells can reduce skin contraction in an in vitro tissue engineered full thickness skin model |
| title_full_unstemmed | Adipose tissue and adipose-derived stromal cells can reduce skin contraction in an in vitro tissue engineered full thickness skin model |
| title_short | Adipose tissue and adipose-derived stromal cells can reduce skin contraction in an in vitro tissue engineered full thickness skin model |
| title_sort | adipose tissue and adipose derived stromal cells can reduce skin contraction in an in vitro tissue engineered full thickness skin model |
| topic | Skin contraction adipose tissue fat grafting adipose-derived stromal cells contracture |
| url | https://www.tandfonline.com/doi/10.1080/21623945.2025.2473367 |
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