Multifunctional Boron‐based 2D Nanoplatforms Ameliorate Severe Respiratory Inflammation by Targeting Multiple Inflammatory Mediators
Abstract Effective management of serious respiratory diseases, such as asthma and recalcitrant rhinitis, remains a global challenge. Here, it is shown that induced sputum supernatants (ISS) from patients with asthma contain higher levels of cell‐free DNA (cfDNA) compared to that of healthy volunteer...
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| Format: | Article |
| Language: | English |
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Wiley
2025-04-01
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| Series: | Advanced Science |
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| Online Access: | https://doi.org/10.1002/advs.202412626 |
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| author | Changyi Xu Ming Liu Xinran Xie Zhixin Li Yuefei Zhu Yang Ye Mengya Du Suhua Hu Tianrun Liu Yubiao Guo Weiping Wen Huanliang Liu Zhaoxu Tu |
| author_facet | Changyi Xu Ming Liu Xinran Xie Zhixin Li Yuefei Zhu Yang Ye Mengya Du Suhua Hu Tianrun Liu Yubiao Guo Weiping Wen Huanliang Liu Zhaoxu Tu |
| author_sort | Changyi Xu |
| collection | DOAJ |
| description | Abstract Effective management of serious respiratory diseases, such as asthma and recalcitrant rhinitis, remains a global challenge. Here, it is shown that induced sputum supernatants (ISS) from patients with asthma contain higher levels of cell‐free DNA (cfDNA) compared to that of healthy volunteers. Although cfDNA scavenging strategies have been developed for inflammation modulation in previous studies, this fall short in clinical settings due to the excessive neutrophil extracellular trap (NET) formation, reactive oxygen and nitrogen species (RONS) and bacterial infections in injured airway tissues. Based on this, a multifunctional boron‐based 2D nanoplatform B‐PM is designed by coating boron nanosheets (B‐NS) with polyamidoamine generation 1 (PG1) dendrimer, which can simultaneously target cfDNA, NETs, RONS, and bacteria. The effects of B‐PM in promoting mucosal repair, reducing airway inflammation, and mucus production have been demonstrated in model mice, and the therapeutic effect is superior to dexamethasone. Furthermore, flow cytometry with clustering analysis and transcriptome analysis with RNA‐sequencing are adopted to comprehensively evaluate the in vivo anti‐inflammation therapeutic effects. These findings emphasize the significance of a multi‐targeting strategy to modulate dysregulated inflammation and highlight multifunctional boron‐based 2D nanoplatforms for the amelioration of respiratory inflammatory diseases. |
| format | Article |
| id | doaj-art-cba10166fc6e43f289da999301abc720 |
| institution | OA Journals |
| issn | 2198-3844 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | Wiley |
| record_format | Article |
| series | Advanced Science |
| spelling | doaj-art-cba10166fc6e43f289da999301abc7202025-08-20T01:51:39ZengWileyAdvanced Science2198-38442025-04-011213n/an/a10.1002/advs.202412626Multifunctional Boron‐based 2D Nanoplatforms Ameliorate Severe Respiratory Inflammation by Targeting Multiple Inflammatory MediatorsChangyi Xu0Ming Liu1Xinran Xie2Zhixin Li3Yuefei Zhu4Yang Ye5Mengya Du6Suhua Hu7Tianrun Liu8Yubiao Guo9Weiping Wen10Huanliang Liu11Zhaoxu Tu12Department of Otolaryngology The Sixth Affiliated Hospital Sun Yat‐sen University Guangzhou Guangdong 510655 ChinaDepartment of Otolaryngology The Sixth Affiliated Hospital Sun Yat‐sen University Guangzhou Guangdong 510655 ChinaDepartment of Otolaryngology The Sixth Affiliated Hospital Sun Yat‐sen University Guangzhou Guangdong 510655 ChinaDepartment of Otolaryngology The Sixth Affiliated Hospital Sun Yat‐sen University Guangzhou Guangdong 510655 ChinaDepartment of Biomedical Engineering Columbia University New York 10027 USADepartment of Otolaryngology The Sixth Affiliated Hospital Sun Yat‐sen University Guangzhou Guangdong 510655 ChinaDepartment of Otolaryngology The Sixth Affiliated Hospital Sun Yat‐sen University Guangzhou Guangdong 510655 ChinaDepartment of Clinical Laboratory The Sixth Affiliated Hospital Sun Yat‐sen University Guangzhou Guangdong 510655 ChinaDepartment of Otolaryngology The Sixth Affiliated Hospital Sun Yat‐sen University Guangzhou Guangdong 510655 ChinaDepartment of Pulmonary and Critical Care Medicine The First Affiliated Hospital Sun Yat‐Sen University Guangzhou Guangdong 510655 ChinaDepartment of Otolaryngology The Sixth Affiliated Hospital Sun Yat‐sen University Guangzhou Guangdong 510655 ChinaDepartment of Clinical Laboratory The Sixth Affiliated Hospital Sun Yat‐sen University Guangzhou Guangdong 510655 ChinaDepartment of Otolaryngology The Sixth Affiliated Hospital Sun Yat‐sen University Guangzhou Guangdong 510655 ChinaAbstract Effective management of serious respiratory diseases, such as asthma and recalcitrant rhinitis, remains a global challenge. Here, it is shown that induced sputum supernatants (ISS) from patients with asthma contain higher levels of cell‐free DNA (cfDNA) compared to that of healthy volunteers. Although cfDNA scavenging strategies have been developed for inflammation modulation in previous studies, this fall short in clinical settings due to the excessive neutrophil extracellular trap (NET) formation, reactive oxygen and nitrogen species (RONS) and bacterial infections in injured airway tissues. Based on this, a multifunctional boron‐based 2D nanoplatform B‐PM is designed by coating boron nanosheets (B‐NS) with polyamidoamine generation 1 (PG1) dendrimer, which can simultaneously target cfDNA, NETs, RONS, and bacteria. The effects of B‐PM in promoting mucosal repair, reducing airway inflammation, and mucus production have been demonstrated in model mice, and the therapeutic effect is superior to dexamethasone. Furthermore, flow cytometry with clustering analysis and transcriptome analysis with RNA‐sequencing are adopted to comprehensively evaluate the in vivo anti‐inflammation therapeutic effects. These findings emphasize the significance of a multi‐targeting strategy to modulate dysregulated inflammation and highlight multifunctional boron‐based 2D nanoplatforms for the amelioration of respiratory inflammatory diseases.https://doi.org/10.1002/advs.202412626bacterial infectionboron‐based nanosheetsneutrophil extracellular trapsreactive oxygen and nitric speciesrespiratory inflammation |
| spellingShingle | Changyi Xu Ming Liu Xinran Xie Zhixin Li Yuefei Zhu Yang Ye Mengya Du Suhua Hu Tianrun Liu Yubiao Guo Weiping Wen Huanliang Liu Zhaoxu Tu Multifunctional Boron‐based 2D Nanoplatforms Ameliorate Severe Respiratory Inflammation by Targeting Multiple Inflammatory Mediators Advanced Science bacterial infection boron‐based nanosheets neutrophil extracellular traps reactive oxygen and nitric species respiratory inflammation |
| title | Multifunctional Boron‐based 2D Nanoplatforms Ameliorate Severe Respiratory Inflammation by Targeting Multiple Inflammatory Mediators |
| title_full | Multifunctional Boron‐based 2D Nanoplatforms Ameliorate Severe Respiratory Inflammation by Targeting Multiple Inflammatory Mediators |
| title_fullStr | Multifunctional Boron‐based 2D Nanoplatforms Ameliorate Severe Respiratory Inflammation by Targeting Multiple Inflammatory Mediators |
| title_full_unstemmed | Multifunctional Boron‐based 2D Nanoplatforms Ameliorate Severe Respiratory Inflammation by Targeting Multiple Inflammatory Mediators |
| title_short | Multifunctional Boron‐based 2D Nanoplatforms Ameliorate Severe Respiratory Inflammation by Targeting Multiple Inflammatory Mediators |
| title_sort | multifunctional boron based 2d nanoplatforms ameliorate severe respiratory inflammation by targeting multiple inflammatory mediators |
| topic | bacterial infection boron‐based nanosheets neutrophil extracellular traps reactive oxygen and nitric species respiratory inflammation |
| url | https://doi.org/10.1002/advs.202412626 |
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