Development of a one-step multiplex RT-qPCR method for rapid detection of bovine diarrhea viruses

Development of a one-step multiplex RT-qPCR method for rapid detection of bovine diarrhea viruses

IntroductionViral calf diarrhea poses a significant challenge to the cattle industry worldwide due to its high morbidity and mortality rates, leading to substantial economic losses. The clinical symptoms associated with various diarrhea pathogens often overlap, complicating accurate diagnosis; thus,...

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Main Authors: Dequan Yang, Li Ma, Zhongping Yang, Xianchao Yang, Jian Wang, Houbin Ju, Chunguang Lu, Yonggang Weng, Heping Zhao, Haixiao Shen, Xin Li, Feifei Ge, Xiaoxu Wang, Xiujuan Wu, Meng Xiang, Guidan Feng, Congsheng Tang, Shixin Huang, Hongjin Zhao
Format: Article
Language:English
Published: Frontiers Media S.A. 2025-01-01
Series:Frontiers in Cellular and Infection Microbiology
Subjects:
calf diarrhea
multiplex real-time quantitative PCR (mRT-qPCR)
clinical detection
method
epidemiological surveillance
Online Access:https://www.frontiersin.org/articles/10.3389/fcimb.2024.1540710/full
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