Spontaneous Cell Detachment from Temperature Gradients: Getting the Method Ready for Antimicrobial Drug Testing at Cell Culture Level
Spontaneous cell detachment describes an effect in which eukaryotic cells first sediment onto a heated chip and then detach from it spontaneously and collectively after a sharply defined dwell time <i>t<sub>d</sub></i>. This behavior is triggered by the temperature gradient b...
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2025-05-01
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| Online Access: | https://www.mdpi.com/1424-8220/25/9/2902 |
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| author | Csongor Tibor Urbán Soroush Bakhshi Sichani Gabriela Ueda Modaffore Christ Glorieux Jonas Gruber Derick Yongabi Minne Paul Lettinga Patrick Wagner |
| author_facet | Csongor Tibor Urbán Soroush Bakhshi Sichani Gabriela Ueda Modaffore Christ Glorieux Jonas Gruber Derick Yongabi Minne Paul Lettinga Patrick Wagner |
| author_sort | Csongor Tibor Urbán |
| collection | DOAJ |
| description | Spontaneous cell detachment describes an effect in which eukaryotic cells first sediment onto a heated chip and then detach from it spontaneously and collectively after a sharply defined dwell time <i>t<sub>d</sub></i>. This behavior is triggered by the temperature gradient between the chip and the colder supernatant liquid. Notably, <i>t<sub>d</sub></i> allows distinguishing between different yeast strains and cancer-cell lines. At the same time, it also varies in the presence of nutrients and cytotoxins, suggesting an added value of this method for pharmacological studies. In the present work, we study the role of fluid convection on the detachment of yeast cells experimentally and by simulations using a sample compartment with a variable aspect ratio. Hereby, we found that the absolute chip temperature, the strength of the temperature gradient and the number of cells inside the sample compartment all affect the dwell time <i>t<sub>d</sub></i>. To demonstrate the concept, we show that the spontaneous-detachment method can measure the impact of an antibiotic and an antiseptic drug on yeast cultures and corroborate this with reference assays. |
| format | Article |
| id | doaj-art-cb8e1066e5e04731bab77e658df4fda9 |
| institution | Kabale University |
| issn | 1424-8220 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | MDPI AG |
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| series | Sensors |
| spelling | doaj-art-cb8e1066e5e04731bab77e658df4fda92025-08-20T03:52:57ZengMDPI AGSensors1424-82202025-05-01259290210.3390/s25092902Spontaneous Cell Detachment from Temperature Gradients: Getting the Method Ready for Antimicrobial Drug Testing at Cell Culture LevelCsongor Tibor Urbán0Soroush Bakhshi Sichani1Gabriela Ueda Modaffore2Christ Glorieux3Jonas Gruber4Derick Yongabi5Minne Paul Lettinga6Patrick Wagner7Laboratory for Soft Matter and Biophysics ZMB, Department of Physics and Astronomy, KU Leuven, Celestijnenlaan 200 D, B-3001 Leuven, BelgiumLaboratory for Soft Matter and Biophysics ZMB, Department of Physics and Astronomy, KU Leuven, Celestijnenlaan 200 D, B-3001 Leuven, BelgiumLaboratory for Soft Matter and Biophysics ZMB, Department of Physics and Astronomy, KU Leuven, Celestijnenlaan 200 D, B-3001 Leuven, BelgiumLaboratory for Soft Matter and Biophysics ZMB, Department of Physics and Astronomy, KU Leuven, Celestijnenlaan 200 D, B-3001 Leuven, BelgiumDepartment of Fundamental Chemistry, Institute of Chemistry, University of São Paulo, Prof. Lineu Prestes Ave., 748, São Paulo 05508-000, SP, BrazilLaboratory for Soft Matter and Biophysics ZMB, Department of Physics and Astronomy, KU Leuven, Celestijnenlaan 200 D, B-3001 Leuven, BelgiumLaboratory for Soft Matter and Biophysics ZMB, Department of Physics and Astronomy, KU Leuven, Celestijnenlaan 200 D, B-3001 Leuven, BelgiumLaboratory for Soft Matter and Biophysics ZMB, Department of Physics and Astronomy, KU Leuven, Celestijnenlaan 200 D, B-3001 Leuven, BelgiumSpontaneous cell detachment describes an effect in which eukaryotic cells first sediment onto a heated chip and then detach from it spontaneously and collectively after a sharply defined dwell time <i>t<sub>d</sub></i>. This behavior is triggered by the temperature gradient between the chip and the colder supernatant liquid. Notably, <i>t<sub>d</sub></i> allows distinguishing between different yeast strains and cancer-cell lines. At the same time, it also varies in the presence of nutrients and cytotoxins, suggesting an added value of this method for pharmacological studies. In the present work, we study the role of fluid convection on the detachment of yeast cells experimentally and by simulations using a sample compartment with a variable aspect ratio. Hereby, we found that the absolute chip temperature, the strength of the temperature gradient and the number of cells inside the sample compartment all affect the dwell time <i>t<sub>d</sub></i>. To demonstrate the concept, we show that the spontaneous-detachment method can measure the impact of an antibiotic and an antiseptic drug on yeast cultures and corroborate this with reference assays.https://www.mdpi.com/1424-8220/25/9/2902cell-based biosensors<i>S. cerevisiae</i>heat-transfer method HTMtemperature gradientsspontaneous cell detachmentmicrofluidic aspect ratio |
| spellingShingle | Csongor Tibor Urbán Soroush Bakhshi Sichani Gabriela Ueda Modaffore Christ Glorieux Jonas Gruber Derick Yongabi Minne Paul Lettinga Patrick Wagner Spontaneous Cell Detachment from Temperature Gradients: Getting the Method Ready for Antimicrobial Drug Testing at Cell Culture Level Sensors cell-based biosensors <i>S. cerevisiae</i> heat-transfer method HTM temperature gradients spontaneous cell detachment microfluidic aspect ratio |
| title | Spontaneous Cell Detachment from Temperature Gradients: Getting the Method Ready for Antimicrobial Drug Testing at Cell Culture Level |
| title_full | Spontaneous Cell Detachment from Temperature Gradients: Getting the Method Ready for Antimicrobial Drug Testing at Cell Culture Level |
| title_fullStr | Spontaneous Cell Detachment from Temperature Gradients: Getting the Method Ready for Antimicrobial Drug Testing at Cell Culture Level |
| title_full_unstemmed | Spontaneous Cell Detachment from Temperature Gradients: Getting the Method Ready for Antimicrobial Drug Testing at Cell Culture Level |
| title_short | Spontaneous Cell Detachment from Temperature Gradients: Getting the Method Ready for Antimicrobial Drug Testing at Cell Culture Level |
| title_sort | spontaneous cell detachment from temperature gradients getting the method ready for antimicrobial drug testing at cell culture level |
| topic | cell-based biosensors <i>S. cerevisiae</i> heat-transfer method HTM temperature gradients spontaneous cell detachment microfluidic aspect ratio |
| url | https://www.mdpi.com/1424-8220/25/9/2902 |
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