Mining public genomic databases for novel lanthipeptides: a case study on Bacillus amyloliquefaciens
The increasing prevalence of drug-resistant bacteria has prompted the search for alternative approaches to address bacterial infections. Lanthipeptides, which are small molecule peptides, are biosynthesized and post-translationally modified by ribosomes in gram-positive bacteria. These compounds pos...
Saved in:
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2024-12-01
|
| Series: | Biotechnology & Biotechnological Equipment |
| Subjects: | |
| Online Access: | https://www.tandfonline.com/doi/10.1080/13102818.2024.2402447 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850163737982402560 |
|---|---|
| author | Wenya Zhao Yang Zhang Xue Yang Jianuo He Yana Wang Feiyan Zhang Liping Zhang Hongwei Liu |
| author_facet | Wenya Zhao Yang Zhang Xue Yang Jianuo He Yana Wang Feiyan Zhang Liping Zhang Hongwei Liu |
| author_sort | Wenya Zhao |
| collection | DOAJ |
| description | The increasing prevalence of drug-resistant bacteria has prompted the search for alternative approaches to address bacterial infections. Lanthipeptides, which are small molecule peptides, are biosynthesized and post-translationally modified by ribosomes in gram-positive bacteria. These compounds possess distinctive target recognition properties, making them highly potential candidates for combating antimicrobial resistance. Consequently, they hold promising potential as innovative substitutes for traditional antibiotics. We conducted a comprehensive analysis of 65 publicly available Bacillus amyloliquefaciens genomes’ data to explore the potential for lanthipeptide synthesis in this species. We annotated 21 putative precursor peptide genes for these novel lanthipeptides. Through heterologous expression experiments, we successfully obtained a novel lanthipeptide named amyA3. We accomplished a novel co-transformation strategy by introducing the synthetic enzyme obtained from B. amyloliquefaciens WS-8 into the E. coli BL21 (DE3) strain along with the heterologous precursor peptide amyA3. Through mass spectrometry analysis, we confirmed the successful modification of amyA3 by the synthetic enzyme derived from B. amyloliquefaciens WS-8, which represents a significant breakthrough in this research field. The identification and characterization of the lanthipeptide amyA3 along with the elucidation of its biosynthetic pathway provide novel perspectives into the discovery of naturally derived products and the intricate processes of translation and post-translational modifications. |
| format | Article |
| id | doaj-art-cb8a9d4c27f34c34a432533140f7fb2b |
| institution | OA Journals |
| issn | 1310-2818 1314-3530 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Biotechnology & Biotechnological Equipment |
| spelling | doaj-art-cb8a9d4c27f34c34a432533140f7fb2b2025-08-20T02:22:10ZengTaylor & Francis GroupBiotechnology & Biotechnological Equipment1310-28181314-35302024-12-0138110.1080/13102818.2024.2402447Mining public genomic databases for novel lanthipeptides: a case study on Bacillus amyloliquefaciensWenya Zhao0Yang Zhang1Xue Yang2Jianuo He3Yana Wang4Feiyan Zhang5Liping Zhang6Hongwei Liu7Institute of Biology, Hebei Academy of Science, Shijiazhuang, P.R. ChinaInstitute of Biology, Hebei Academy of Science, Shijiazhuang, P.R. ChinaInstitute of Biology, Hebei Academy of Science, Shijiazhuang, P.R. ChinaInstitute of Biology, Hebei Academy of Science, Shijiazhuang, P.R. ChinaInstitute of Biology, Hebei Academy of Science, Shijiazhuang, P.R. ChinaInstitute of Biology, Hebei Academy of Science, Shijiazhuang, P.R. ChinaInstitute of Biology, Hebei Academy of Science, Shijiazhuang, P.R. ChinaInstitute of Biology, Hebei Academy of Science, Shijiazhuang, P.R. ChinaThe increasing prevalence of drug-resistant bacteria has prompted the search for alternative approaches to address bacterial infections. Lanthipeptides, which are small molecule peptides, are biosynthesized and post-translationally modified by ribosomes in gram-positive bacteria. These compounds possess distinctive target recognition properties, making them highly potential candidates for combating antimicrobial resistance. Consequently, they hold promising potential as innovative substitutes for traditional antibiotics. We conducted a comprehensive analysis of 65 publicly available Bacillus amyloliquefaciens genomes’ data to explore the potential for lanthipeptide synthesis in this species. We annotated 21 putative precursor peptide genes for these novel lanthipeptides. Through heterologous expression experiments, we successfully obtained a novel lanthipeptide named amyA3. We accomplished a novel co-transformation strategy by introducing the synthetic enzyme obtained from B. amyloliquefaciens WS-8 into the E. coli BL21 (DE3) strain along with the heterologous precursor peptide amyA3. Through mass spectrometry analysis, we confirmed the successful modification of amyA3 by the synthetic enzyme derived from B. amyloliquefaciens WS-8, which represents a significant breakthrough in this research field. The identification and characterization of the lanthipeptide amyA3 along with the elucidation of its biosynthetic pathway provide novel perspectives into the discovery of naturally derived products and the intricate processes of translation and post-translational modifications.https://www.tandfonline.com/doi/10.1080/13102818.2024.2402447LanthipeptideBacillus amyloliquefaciensheterologous expressiongenome mininggene cluster |
| spellingShingle | Wenya Zhao Yang Zhang Xue Yang Jianuo He Yana Wang Feiyan Zhang Liping Zhang Hongwei Liu Mining public genomic databases for novel lanthipeptides: a case study on Bacillus amyloliquefaciens Biotechnology & Biotechnological Equipment Lanthipeptide Bacillus amyloliquefaciens heterologous expression genome mining gene cluster |
| title | Mining public genomic databases for novel lanthipeptides: a case study on Bacillus amyloliquefaciens |
| title_full | Mining public genomic databases for novel lanthipeptides: a case study on Bacillus amyloliquefaciens |
| title_fullStr | Mining public genomic databases for novel lanthipeptides: a case study on Bacillus amyloliquefaciens |
| title_full_unstemmed | Mining public genomic databases for novel lanthipeptides: a case study on Bacillus amyloliquefaciens |
| title_short | Mining public genomic databases for novel lanthipeptides: a case study on Bacillus amyloliquefaciens |
| title_sort | mining public genomic databases for novel lanthipeptides a case study on bacillus amyloliquefaciens |
| topic | Lanthipeptide Bacillus amyloliquefaciens heterologous expression genome mining gene cluster |
| url | https://www.tandfonline.com/doi/10.1080/13102818.2024.2402447 |
| work_keys_str_mv | AT wenyazhao miningpublicgenomicdatabasesfornovellanthipeptidesacasestudyonbacillusamyloliquefaciens AT yangzhang miningpublicgenomicdatabasesfornovellanthipeptidesacasestudyonbacillusamyloliquefaciens AT xueyang miningpublicgenomicdatabasesfornovellanthipeptidesacasestudyonbacillusamyloliquefaciens AT jianuohe miningpublicgenomicdatabasesfornovellanthipeptidesacasestudyonbacillusamyloliquefaciens AT yanawang miningpublicgenomicdatabasesfornovellanthipeptidesacasestudyonbacillusamyloliquefaciens AT feiyanzhang miningpublicgenomicdatabasesfornovellanthipeptidesacasestudyonbacillusamyloliquefaciens AT lipingzhang miningpublicgenomicdatabasesfornovellanthipeptidesacasestudyonbacillusamyloliquefaciens AT hongweiliu miningpublicgenomicdatabasesfornovellanthipeptidesacasestudyonbacillusamyloliquefaciens |