Benzyl Benzoate Isolation from <i>Acridocarpus smeathmannii</i> (DC.) Guill. & Perr Roots and Its Bioactivity on Human Prostate Smooth Muscle Contractions
<b>Background/Objectives:</b> This study is the first report on isolating a natural benzyl benzoate (nBB) from <i>Acridocarpus smeathmannii</i> (DC.) Guill. & Perr roots. <b>Methods</b>: The structure was verified using GC-MS, HPLC-UV-VIS, and two-dimensional...
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2025-05-01
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| author | Oluwafemi Ezekiel Kale Iskander Rauanov Claudia Huber Alexander Tamalunas Christian G. Stief Wolfgang Eisenreich Martin Hennenberg |
| author_facet | Oluwafemi Ezekiel Kale Iskander Rauanov Claudia Huber Alexander Tamalunas Christian G. Stief Wolfgang Eisenreich Martin Hennenberg |
| author_sort | Oluwafemi Ezekiel Kale |
| collection | DOAJ |
| description | <b>Background/Objectives:</b> This study is the first report on isolating a natural benzyl benzoate (nBB) from <i>Acridocarpus smeathmannii</i> (DC.) Guill. & Perr roots. <b>Methods</b>: The structure was verified using GC-MS, HPLC-UV-VIS, and two-dimensional NMR. Since it is known for its vasodilatory and anti-spasmolytic actions, we investigated the biological effects of nBB on human prostate smooth tissue (rPx) obtained from a radical prostatectomy. For this purpose, rPx was incubated with nBB (0.05, 0.25, or 0.5 µM) in an organ bath, and then cumulative concentration–response curves were constructed for adrenergic agonists and electrical field stimulation (EFS). <b>Results</b>: Adding the various concentrations, nBB showed potential inhibition during agonist-induced contractions (0.1–100 µM). Also, neurogenic contractions of rPx by EFS (2–32 Hz) were reduced by up to 57%. <b>Conclusions</b>: Overall, this study reports on an efficient protocol of nBB isolation from <i>A. smeathmannii</i> and its contractility effects on human prostate smooth muscle. Potentially, this could contribute to the natural production of BB from <i>A. smeathmannii</i> species while giving it evolutionary recognition. However, since BB influences prostate smooth muscle contractility, caution in patients taking herbal supplements containing nBB is essential, as this may play a role in contributing to the symptoms of urinary tract conditions. |
| format | Article |
| id | doaj-art-cb8a5abaae534be5a9cef48bf19b374b |
| institution | DOAJ |
| issn | 1424-8247 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pharmaceuticals |
| spelling | doaj-art-cb8a5abaae534be5a9cef48bf19b374b2025-08-20T03:14:29ZengMDPI AGPharmaceuticals1424-82472025-05-0118568710.3390/ph18050687Benzyl Benzoate Isolation from <i>Acridocarpus smeathmannii</i> (DC.) Guill. & Perr Roots and Its Bioactivity on Human Prostate Smooth Muscle ContractionsOluwafemi Ezekiel Kale0Iskander Rauanov1Claudia Huber2Alexander Tamalunas3Christian G. Stief4Wolfgang Eisenreich5Martin Hennenberg6Department of Pharmacology and Therapeutics, Faculty of Basic Medical Sciences, Olabisi Onabanjo University, Sagamu Campus, Ago-Iwoye 2001, Ogun State, NigeriaBavarian NMR Center—Structural Membrane Biochemistry, Department of Chemistry, Technical University of Munich, 85748 Garching, GermanyBavarian NMR Center—Structural Membrane Biochemistry, Department of Chemistry, Technical University of Munich, 85748 Garching, GermanyDepartment of Urology, LMU University Hospital, LMU Munich, 80336 Munich, GermanyDepartment of Urology, LMU University Hospital, LMU Munich, 80336 Munich, GermanyBavarian NMR Center—Structural Membrane Biochemistry, Department of Chemistry, Technical University of Munich, 85748 Garching, GermanyDepartment of Urology, LMU University Hospital, LMU Munich, 80336 Munich, Germany<b>Background/Objectives:</b> This study is the first report on isolating a natural benzyl benzoate (nBB) from <i>Acridocarpus smeathmannii</i> (DC.) Guill. & Perr roots. <b>Methods</b>: The structure was verified using GC-MS, HPLC-UV-VIS, and two-dimensional NMR. Since it is known for its vasodilatory and anti-spasmolytic actions, we investigated the biological effects of nBB on human prostate smooth tissue (rPx) obtained from a radical prostatectomy. For this purpose, rPx was incubated with nBB (0.05, 0.25, or 0.5 µM) in an organ bath, and then cumulative concentration–response curves were constructed for adrenergic agonists and electrical field stimulation (EFS). <b>Results</b>: Adding the various concentrations, nBB showed potential inhibition during agonist-induced contractions (0.1–100 µM). Also, neurogenic contractions of rPx by EFS (2–32 Hz) were reduced by up to 57%. <b>Conclusions</b>: Overall, this study reports on an efficient protocol of nBB isolation from <i>A. smeathmannii</i> and its contractility effects on human prostate smooth muscle. Potentially, this could contribute to the natural production of BB from <i>A. smeathmannii</i> species while giving it evolutionary recognition. However, since BB influences prostate smooth muscle contractility, caution in patients taking herbal supplements containing nBB is essential, as this may play a role in contributing to the symptoms of urinary tract conditions.https://www.mdpi.com/1424-8247/18/5/687bioactivity-guided studynatural benzyl benzoate<i>Acridocarpus smeathmannii</i>prostate smooth muscledrug discovery |
| spellingShingle | Oluwafemi Ezekiel Kale Iskander Rauanov Claudia Huber Alexander Tamalunas Christian G. Stief Wolfgang Eisenreich Martin Hennenberg Benzyl Benzoate Isolation from <i>Acridocarpus smeathmannii</i> (DC.) Guill. & Perr Roots and Its Bioactivity on Human Prostate Smooth Muscle Contractions Pharmaceuticals bioactivity-guided study natural benzyl benzoate <i>Acridocarpus smeathmannii</i> prostate smooth muscle drug discovery |
| title | Benzyl Benzoate Isolation from <i>Acridocarpus smeathmannii</i> (DC.) Guill. & Perr Roots and Its Bioactivity on Human Prostate Smooth Muscle Contractions |
| title_full | Benzyl Benzoate Isolation from <i>Acridocarpus smeathmannii</i> (DC.) Guill. & Perr Roots and Its Bioactivity on Human Prostate Smooth Muscle Contractions |
| title_fullStr | Benzyl Benzoate Isolation from <i>Acridocarpus smeathmannii</i> (DC.) Guill. & Perr Roots and Its Bioactivity on Human Prostate Smooth Muscle Contractions |
| title_full_unstemmed | Benzyl Benzoate Isolation from <i>Acridocarpus smeathmannii</i> (DC.) Guill. & Perr Roots and Its Bioactivity on Human Prostate Smooth Muscle Contractions |
| title_short | Benzyl Benzoate Isolation from <i>Acridocarpus smeathmannii</i> (DC.) Guill. & Perr Roots and Its Bioactivity on Human Prostate Smooth Muscle Contractions |
| title_sort | benzyl benzoate isolation from i acridocarpus smeathmannii i dc guill perr roots and its bioactivity on human prostate smooth muscle contractions |
| topic | bioactivity-guided study natural benzyl benzoate <i>Acridocarpus smeathmannii</i> prostate smooth muscle drug discovery |
| url | https://www.mdpi.com/1424-8247/18/5/687 |
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