Unveiling the molecular blueprint of SKP-SCs-mediated tissue engineering-enhanced neuroregeneration
Abstract Peripheral nerve injury poses a significant challenge to the nervous system’s regenerative capacity. We previously described a novel approach to construct a chitosan/silk fibroin nerve graft with skin-derived precursor-induced Schwann cells (SKP-SCs). This graft has been shown to promote sc...
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| Format: | Article |
| Language: | English |
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BMC
2024-12-01
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| Series: | Journal of Nanobiotechnology |
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| Online Access: | https://doi.org/10.1186/s12951-024-03076-1 |
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| author | Hui Zhu Ying Wang Siyuan Xu Yunjian Song Yifan Li Yiting Wang Qiuwen Sun Muyuan Tong Tianyi Huang Yulin Pan Hongkui Wang Xi Xu Chengbin Xue |
| author_facet | Hui Zhu Ying Wang Siyuan Xu Yunjian Song Yifan Li Yiting Wang Qiuwen Sun Muyuan Tong Tianyi Huang Yulin Pan Hongkui Wang Xi Xu Chengbin Xue |
| author_sort | Hui Zhu |
| collection | DOAJ |
| description | Abstract Peripheral nerve injury poses a significant challenge to the nervous system’s regenerative capacity. We previously described a novel approach to construct a chitosan/silk fibroin nerve graft with skin-derived precursor-induced Schwann cells (SKP-SCs). This graft has been shown to promote sciatic nerve regeneration and functional restoration to a level comparable to that achieved by autologous nerve grafts, as evidenced by behavioral, histological, and electrophysiological assessments. However, the underlying molecular mechanisms based on SKP-SCs mediated tissue engineering-aid regeneration remain elusive. In the present work, we systematically identified gene modules associated with the differentiation of SKPs into SCs by employing weighted gene co-expression network analysis (WGCNA). By integrating transcriptomic data from the regenerated nerve segment, we constructed a network that delineated the molecular signatures of TENG aid neuroregeneration. Subsequent quantitative PCR (qPCR) validation was performed to substantiate the WGCNA findings. Our WGCNA approach revealed a robust molecular landscape, highlighting hub genes pivotal for tissue engineering-aid regeneration. Notably, the upregulation of specific genes was observed to coincide with the acquisition of SC characteristics. The qPCR validation confirmed the expression patterns of these genes, underscoring their role in promoting neuroregeneration. The current study harnesses the power of WGCNA to elucidate the molecular blueprint governing tissue engineering-aid regeneration. The identified gene modules and validated targets offer novel insights into the cellular and molecular underpinnings of tissue engineering-augmented neuroregeneration. These findings pave the way for developing targeted therapeutics and advanced tissue engineering grafts to enhance peripheral nerve repair. |
| format | Article |
| id | doaj-art-cb6bb83ad0714873a7acfbcd3c7cd046 |
| institution | DOAJ |
| issn | 1477-3155 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Nanobiotechnology |
| spelling | doaj-art-cb6bb83ad0714873a7acfbcd3c7cd0462025-08-20T02:39:40ZengBMCJournal of Nanobiotechnology1477-31552024-12-0122111810.1186/s12951-024-03076-1Unveiling the molecular blueprint of SKP-SCs-mediated tissue engineering-enhanced neuroregenerationHui Zhu0Ying Wang1Siyuan Xu2Yunjian Song3Yifan Li4Yiting Wang5Qiuwen Sun6Muyuan Tong7Tianyi Huang8Yulin Pan9Hongkui Wang10Xi Xu11Chengbin Xue12Research Center of Clinical Medicine, Affiliated Hospital of Nantong University, Key Laboratory of Neuroregeneration of Jiangsu and Ministry of Education, Co-innovation Center of Neuroregeneration, NMPA Key Laboratory for Research and Evaluation of Tissue Engineering Technology Products, Nantong UniversityMedical School of Nantong UniversityMedical School of Nantong UniversityMedical School of Nantong UniversityMedical School of Nantong UniversityMedical School of Nantong UniversityMedical School of Nantong UniversityMedical School of Nantong UniversityMedical School of Nantong UniversityMedical School of Nantong UniversityResearch Center of Clinical Medicine, Affiliated Hospital of Nantong University, Key Laboratory of Neuroregeneration of Jiangsu and Ministry of Education, Co-innovation Center of Neuroregeneration, NMPA Key Laboratory for Research and Evaluation of Tissue Engineering Technology Products, Nantong UniversityResearch Center of Clinical Medicine, Affiliated Hospital of Nantong University, Key Laboratory of Neuroregeneration of Jiangsu and Ministry of Education, Co-innovation Center of Neuroregeneration, NMPA Key Laboratory for Research and Evaluation of Tissue Engineering Technology Products, Nantong UniversityResearch Center of Clinical Medicine, Affiliated Hospital of Nantong University, Key Laboratory of Neuroregeneration of Jiangsu and Ministry of Education, Co-innovation Center of Neuroregeneration, NMPA Key Laboratory for Research and Evaluation of Tissue Engineering Technology Products, Nantong UniversityAbstract Peripheral nerve injury poses a significant challenge to the nervous system’s regenerative capacity. We previously described a novel approach to construct a chitosan/silk fibroin nerve graft with skin-derived precursor-induced Schwann cells (SKP-SCs). This graft has been shown to promote sciatic nerve regeneration and functional restoration to a level comparable to that achieved by autologous nerve grafts, as evidenced by behavioral, histological, and electrophysiological assessments. However, the underlying molecular mechanisms based on SKP-SCs mediated tissue engineering-aid regeneration remain elusive. In the present work, we systematically identified gene modules associated with the differentiation of SKPs into SCs by employing weighted gene co-expression network analysis (WGCNA). By integrating transcriptomic data from the regenerated nerve segment, we constructed a network that delineated the molecular signatures of TENG aid neuroregeneration. Subsequent quantitative PCR (qPCR) validation was performed to substantiate the WGCNA findings. Our WGCNA approach revealed a robust molecular landscape, highlighting hub genes pivotal for tissue engineering-aid regeneration. Notably, the upregulation of specific genes was observed to coincide with the acquisition of SC characteristics. The qPCR validation confirmed the expression patterns of these genes, underscoring their role in promoting neuroregeneration. The current study harnesses the power of WGCNA to elucidate the molecular blueprint governing tissue engineering-aid regeneration. The identified gene modules and validated targets offer novel insights into the cellular and molecular underpinnings of tissue engineering-augmented neuroregeneration. These findings pave the way for developing targeted therapeutics and advanced tissue engineering grafts to enhance peripheral nerve repair.https://doi.org/10.1186/s12951-024-03076-1Tissue engineeringPeripheral nerve regenerationSciatic nerve injurySchwann cellsSkin-derived precursorsWeighted gene co-expression network analysis (WGCNA) |
| spellingShingle | Hui Zhu Ying Wang Siyuan Xu Yunjian Song Yifan Li Yiting Wang Qiuwen Sun Muyuan Tong Tianyi Huang Yulin Pan Hongkui Wang Xi Xu Chengbin Xue Unveiling the molecular blueprint of SKP-SCs-mediated tissue engineering-enhanced neuroregeneration Journal of Nanobiotechnology Tissue engineering Peripheral nerve regeneration Sciatic nerve injury Schwann cells Skin-derived precursors Weighted gene co-expression network analysis (WGCNA) |
| title | Unveiling the molecular blueprint of SKP-SCs-mediated tissue engineering-enhanced neuroregeneration |
| title_full | Unveiling the molecular blueprint of SKP-SCs-mediated tissue engineering-enhanced neuroregeneration |
| title_fullStr | Unveiling the molecular blueprint of SKP-SCs-mediated tissue engineering-enhanced neuroregeneration |
| title_full_unstemmed | Unveiling the molecular blueprint of SKP-SCs-mediated tissue engineering-enhanced neuroregeneration |
| title_short | Unveiling the molecular blueprint of SKP-SCs-mediated tissue engineering-enhanced neuroregeneration |
| title_sort | unveiling the molecular blueprint of skp scs mediated tissue engineering enhanced neuroregeneration |
| topic | Tissue engineering Peripheral nerve regeneration Sciatic nerve injury Schwann cells Skin-derived precursors Weighted gene co-expression network analysis (WGCNA) |
| url | https://doi.org/10.1186/s12951-024-03076-1 |
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