Structure of a transcribing Pol II-DSIF-SPT6-U1 snRNP complex
Abstract In eukaryotic cells, splicing occurs predominantly co-transcriptionally, enhancing splicing efficiency and fidelity while introducing an additional layer of regulation over gene expression. RNA polymerase II (Pol II) facilitates co-transcriptional splicing by recruiting the U1 small nuclear...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2025-07-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-025-60979-9 |
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| _version_ | 1849334643946422272 |
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| author | Luojia Zhang Christopher Batters Shintaro Aibara Yuliya Gordiyenko Kristina Žumer Jana Schmitzová Kerstin Maier Patrick Cramer Suyang Zhang |
| author_facet | Luojia Zhang Christopher Batters Shintaro Aibara Yuliya Gordiyenko Kristina Žumer Jana Schmitzová Kerstin Maier Patrick Cramer Suyang Zhang |
| author_sort | Luojia Zhang |
| collection | DOAJ |
| description | Abstract In eukaryotic cells, splicing occurs predominantly co-transcriptionally, enhancing splicing efficiency and fidelity while introducing an additional layer of regulation over gene expression. RNA polymerase II (Pol II) facilitates co-transcriptional splicing by recruiting the U1 small nuclear ribonucleoprotein particle (U1 snRNP) to the nascent transcripts. Here, we report the cryo-electron microscopy structure of a transcribing Pol II-U1 snRNP complex with elongation factors DSIF and SPT6. In addition, our biochemical analysis reveals that the phosphorylated Pol II carboxyl-terminal domain and SPT6 interact directly with U1 snRNP proteins, facilitating its recruitment to the elongation complex. This multivalent interaction between U1 snRNP and the transcription elongation complex may both allow efficient spliceosome assembly and ensure transcription processivity. |
| format | Article |
| id | doaj-art-cb6a9613afe44eccadffe2d74f98a492 |
| institution | Kabale University |
| issn | 2041-1723 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj-art-cb6a9613afe44eccadffe2d74f98a4922025-08-20T03:45:31ZengNature PortfolioNature Communications2041-17232025-07-0116111010.1038/s41467-025-60979-9Structure of a transcribing Pol II-DSIF-SPT6-U1 snRNP complexLuojia Zhang0Christopher Batters1Shintaro Aibara2Yuliya Gordiyenko3Kristina Žumer4Jana Schmitzová5Kerstin Maier6Patrick Cramer7Suyang Zhang8MRC Laboratory of Molecular BiologyMRC Laboratory of Molecular BiologyMax Planck Institute for Multidisciplinary Sciences, Department of Molecular BiologyMRC Laboratory of Molecular BiologyMax Planck Institute for Multidisciplinary Sciences, Department of Molecular BiologyMax Planck Institute for Multidisciplinary Sciences, Department of Molecular BiologyMax Planck Institute for Multidisciplinary Sciences, Department of Molecular BiologyMax Planck Institute for Multidisciplinary Sciences, Department of Molecular BiologyMRC Laboratory of Molecular BiologyAbstract In eukaryotic cells, splicing occurs predominantly co-transcriptionally, enhancing splicing efficiency and fidelity while introducing an additional layer of regulation over gene expression. RNA polymerase II (Pol II) facilitates co-transcriptional splicing by recruiting the U1 small nuclear ribonucleoprotein particle (U1 snRNP) to the nascent transcripts. Here, we report the cryo-electron microscopy structure of a transcribing Pol II-U1 snRNP complex with elongation factors DSIF and SPT6. In addition, our biochemical analysis reveals that the phosphorylated Pol II carboxyl-terminal domain and SPT6 interact directly with U1 snRNP proteins, facilitating its recruitment to the elongation complex. This multivalent interaction between U1 snRNP and the transcription elongation complex may both allow efficient spliceosome assembly and ensure transcription processivity.https://doi.org/10.1038/s41467-025-60979-9 |
| spellingShingle | Luojia Zhang Christopher Batters Shintaro Aibara Yuliya Gordiyenko Kristina Žumer Jana Schmitzová Kerstin Maier Patrick Cramer Suyang Zhang Structure of a transcribing Pol II-DSIF-SPT6-U1 snRNP complex Nature Communications |
| title | Structure of a transcribing Pol II-DSIF-SPT6-U1 snRNP complex |
| title_full | Structure of a transcribing Pol II-DSIF-SPT6-U1 snRNP complex |
| title_fullStr | Structure of a transcribing Pol II-DSIF-SPT6-U1 snRNP complex |
| title_full_unstemmed | Structure of a transcribing Pol II-DSIF-SPT6-U1 snRNP complex |
| title_short | Structure of a transcribing Pol II-DSIF-SPT6-U1 snRNP complex |
| title_sort | structure of a transcribing pol ii dsif spt6 u1 snrnp complex |
| url | https://doi.org/10.1038/s41467-025-60979-9 |
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