Cultivated Mushrooms: A Comparative Study of Antioxidant Activity and Phenolic Content

This study compares the total phenolic content (TPC) and antioxidant activity (DPPH, ABTS, and FRAP assays) of methanolic extracts from five cultivated mushroom species: <i>Lentinula edodes</i> (LE), <i>Pleurotus ostreatus</i> (PO), <i>Agaricus bisporus</i> (AgW a...

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Main Authors: Mafalda Silva, Manuela Lageiro, Ana Cristina Ramos, Fernando H. Reboredo, Elsa M. Gonçalves
Format: Article
Language:English
Published: MDPI AG 2025-01-01
Series:Biology and Life Sciences Forum
Subjects:
Online Access:https://www.mdpi.com/2673-9976/40/1/13
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author Mafalda Silva
Manuela Lageiro
Ana Cristina Ramos
Fernando H. Reboredo
Elsa M. Gonçalves
author_facet Mafalda Silva
Manuela Lageiro
Ana Cristina Ramos
Fernando H. Reboredo
Elsa M. Gonçalves
author_sort Mafalda Silva
collection DOAJ
description This study compares the total phenolic content (TPC) and antioxidant activity (DPPH, ABTS, and FRAP assays) of methanolic extracts from five cultivated mushroom species: <i>Lentinula edodes</i> (LE), <i>Pleurotus ostreatus</i> (PO), <i>Agaricus bisporus</i> (AgW and AgB), and <i>Hericium erinaceus</i> (HE). Phenolic profiles were analyzed by HPLC. AgW demonstrated the highest TPC (46.2 mg GAE/100 g FW) and superior antioxidant activity in DPPH and FRAP assays, highlighting its phenolic compounds as the primary contributors. Catechin and hydroxybenzoic acid were identified as species-specific phenolics in PO and AgW, respectively, contributing to their antioxidant potential. These findings underscore the diversity of antioxidant mechanisms in mushrooms and their potential as natural sources of bioactive compounds.
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series Biology and Life Sciences Forum
spelling doaj-art-cb62d8131a804bf3bdfd1ae8c2a92a712025-08-20T03:26:10ZengMDPI AGBiology and Life Sciences Forum2673-99762025-01-014011310.3390/blsf2024040013Cultivated Mushrooms: A Comparative Study of Antioxidant Activity and Phenolic ContentMafalda Silva0Manuela Lageiro1Ana Cristina Ramos2Fernando H. Reboredo3Elsa M. Gonçalves4INIAV-Instituto Nacional de Investigação Agrária e Veterinária, Unidade de Tecnologia e Inovação, 2780-157 Oeiras, PortugalINIAV-Instituto Nacional de Investigação Agrária e Veterinária, Unidade de Tecnologia e Inovação, 2780-157 Oeiras, PortugalINIAV-Instituto Nacional de Investigação Agrária e Veterinária, Unidade de Tecnologia e Inovação, 2780-157 Oeiras, PortugalGeoBioTec-Geobiociências, Geoengenharias e Geotecnologias, Faculdade de Ciências e Tecnologia (FCT), Universidade NOVA de Lisboa (UNL), 2829-516 Caparica, PortugalINIAV-Instituto Nacional de Investigação Agrária e Veterinária, Unidade de Tecnologia e Inovação, 2780-157 Oeiras, PortugalThis study compares the total phenolic content (TPC) and antioxidant activity (DPPH, ABTS, and FRAP assays) of methanolic extracts from five cultivated mushroom species: <i>Lentinula edodes</i> (LE), <i>Pleurotus ostreatus</i> (PO), <i>Agaricus bisporus</i> (AgW and AgB), and <i>Hericium erinaceus</i> (HE). Phenolic profiles were analyzed by HPLC. AgW demonstrated the highest TPC (46.2 mg GAE/100 g FW) and superior antioxidant activity in DPPH and FRAP assays, highlighting its phenolic compounds as the primary contributors. Catechin and hydroxybenzoic acid were identified as species-specific phenolics in PO and AgW, respectively, contributing to their antioxidant potential. These findings underscore the diversity of antioxidant mechanisms in mushrooms and their potential as natural sources of bioactive compounds.https://www.mdpi.com/2673-9976/40/1/13edible mushroomstotal phenolic contentantioxidant activity
spellingShingle Mafalda Silva
Manuela Lageiro
Ana Cristina Ramos
Fernando H. Reboredo
Elsa M. Gonçalves
Cultivated Mushrooms: A Comparative Study of Antioxidant Activity and Phenolic Content
Biology and Life Sciences Forum
edible mushrooms
total phenolic content
antioxidant activity
title Cultivated Mushrooms: A Comparative Study of Antioxidant Activity and Phenolic Content
title_full Cultivated Mushrooms: A Comparative Study of Antioxidant Activity and Phenolic Content
title_fullStr Cultivated Mushrooms: A Comparative Study of Antioxidant Activity and Phenolic Content
title_full_unstemmed Cultivated Mushrooms: A Comparative Study of Antioxidant Activity and Phenolic Content
title_short Cultivated Mushrooms: A Comparative Study of Antioxidant Activity and Phenolic Content
title_sort cultivated mushrooms a comparative study of antioxidant activity and phenolic content
topic edible mushrooms
total phenolic content
antioxidant activity
url https://www.mdpi.com/2673-9976/40/1/13
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AT manuelalageiro cultivatedmushroomsacomparativestudyofantioxidantactivityandphenoliccontent
AT anacristinaramos cultivatedmushroomsacomparativestudyofantioxidantactivityandphenoliccontent
AT fernandohreboredo cultivatedmushroomsacomparativestudyofantioxidantactivityandphenoliccontent
AT elsamgoncalves cultivatedmushroomsacomparativestudyofantioxidantactivityandphenoliccontent