Preventive role of rottlerin in endotoxin-induced uveitic glaucoma in rats

Abstract Background Rottlerin is a natural polyphenolic compound obtained from dried ripe fruits of Mallotus philippinensis and is primarily used as a PKCδ inhibitor. The effectiveness of rottlerin was checked in the endotoxin-induced glaucoma models, focusing on its impact on intraocular pressure,...

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Main Authors: Nidhi Kumari, Ashmita Das, Nirdesh Kumar, Geeta Singh, Urvashi Jain, Amrita Singh, Surendra H. Bodakhe
Format: Article
Language:English
Published: SpringerOpen 2025-04-01
Series:Future Journal of Pharmaceutical Sciences
Subjects:
Online Access:https://doi.org/10.1186/s43094-025-00798-3
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author Nidhi Kumari
Ashmita Das
Nirdesh Kumar
Geeta Singh
Urvashi Jain
Amrita Singh
Surendra H. Bodakhe
author_facet Nidhi Kumari
Ashmita Das
Nirdesh Kumar
Geeta Singh
Urvashi Jain
Amrita Singh
Surendra H. Bodakhe
author_sort Nidhi Kumari
collection DOAJ
description Abstract Background Rottlerin is a natural polyphenolic compound obtained from dried ripe fruits of Mallotus philippinensis and is primarily used as a PKCδ inhibitor. The effectiveness of rottlerin was checked in the endotoxin-induced glaucoma models, focusing on its impact on intraocular pressure, inflammation, and optic nerve protection. For this study, both pure and extracted rottlerin were used. Extracted rottlerin was characterised by determining its melting point, conducting gas chromatography-mass spectrometry analysis, Nuclear magnetic resonance analysis, and measuring its wavelength and retardation factor. The acute toxicity study was carried out using Organization for Economic Co-operation and development guideline 405 (acute eye irritation test). Result Pure and extracted rottlerin showed decreased intraocular pressure and reduced inflammation, lenticular opacity, and retinal damage. These results are attributed to an increase in antioxidant levels, maintenance of lipid peroxidation, enzyme level (Na+ ATPase, K+ ATPase), ionic balance (Na+, K+), and a decrease in the level of nitric oxide. Conclusion Rottlerin (pure) as well as rottlerin (extracted) has demonstrated potential in managing uveitic glaucoma. Its multifaceted mechanisms not only target inflammation and oxidative stress but also promote tissue regeneration and slow disease progression. This makes rottlerin a promising candidate for further clinical research as a potential treatment for uveitic glaucoma.
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spelling doaj-art-cb5f9b74882e4b0f84c580f511efbfed2025-08-20T03:10:14ZengSpringerOpenFuture Journal of Pharmaceutical Sciences2314-72532025-04-0111111310.1186/s43094-025-00798-3Preventive role of rottlerin in endotoxin-induced uveitic glaucoma in ratsNidhi Kumari0Ashmita Das1Nirdesh Kumar2Geeta Singh3Urvashi Jain4Amrita Singh5Surendra H. Bodakhe6Department of Pharmacy, Guru Ghasidas Vishwavidyalaya (A Central University)Department of Pharmacy, Guru Ghasidas Vishwavidyalaya (A Central University)Department of Pharmacy, Guru Ghasidas Vishwavidyalaya (A Central University)Department of Pharmacy, Guru Ghasidas Vishwavidyalaya (A Central University)Department of Pharmacy, Guru Ghasidas Vishwavidyalaya (A Central University)National Institute of Pharmaceutical Education and ResearchDepartment of Pharmacy, Guru Ghasidas Vishwavidyalaya (A Central University)Abstract Background Rottlerin is a natural polyphenolic compound obtained from dried ripe fruits of Mallotus philippinensis and is primarily used as a PKCδ inhibitor. The effectiveness of rottlerin was checked in the endotoxin-induced glaucoma models, focusing on its impact on intraocular pressure, inflammation, and optic nerve protection. For this study, both pure and extracted rottlerin were used. Extracted rottlerin was characterised by determining its melting point, conducting gas chromatography-mass spectrometry analysis, Nuclear magnetic resonance analysis, and measuring its wavelength and retardation factor. The acute toxicity study was carried out using Organization for Economic Co-operation and development guideline 405 (acute eye irritation test). Result Pure and extracted rottlerin showed decreased intraocular pressure and reduced inflammation, lenticular opacity, and retinal damage. These results are attributed to an increase in antioxidant levels, maintenance of lipid peroxidation, enzyme level (Na+ ATPase, K+ ATPase), ionic balance (Na+, K+), and a decrease in the level of nitric oxide. Conclusion Rottlerin (pure) as well as rottlerin (extracted) has demonstrated potential in managing uveitic glaucoma. Its multifaceted mechanisms not only target inflammation and oxidative stress but also promote tissue regeneration and slow disease progression. This makes rottlerin a promising candidate for further clinical research as a potential treatment for uveitic glaucoma.https://doi.org/10.1186/s43094-025-00798-3RottlerinPKCδ inhibitorLPSInflammationAnti-oxidantsUveitic glaucoma
spellingShingle Nidhi Kumari
Ashmita Das
Nirdesh Kumar
Geeta Singh
Urvashi Jain
Amrita Singh
Surendra H. Bodakhe
Preventive role of rottlerin in endotoxin-induced uveitic glaucoma in rats
Future Journal of Pharmaceutical Sciences
Rottlerin
PKCδ inhibitor
LPS
Inflammation
Anti-oxidants
Uveitic glaucoma
title Preventive role of rottlerin in endotoxin-induced uveitic glaucoma in rats
title_full Preventive role of rottlerin in endotoxin-induced uveitic glaucoma in rats
title_fullStr Preventive role of rottlerin in endotoxin-induced uveitic glaucoma in rats
title_full_unstemmed Preventive role of rottlerin in endotoxin-induced uveitic glaucoma in rats
title_short Preventive role of rottlerin in endotoxin-induced uveitic glaucoma in rats
title_sort preventive role of rottlerin in endotoxin induced uveitic glaucoma in rats
topic Rottlerin
PKCδ inhibitor
LPS
Inflammation
Anti-oxidants
Uveitic glaucoma
url https://doi.org/10.1186/s43094-025-00798-3
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