CPT1A mediates the succinylation of SP5 which activates transcription of PDPK1 to promote the viability and glycolysis of prostate cancer cells
Succinylation modification involves in the progression of human cancers. The present study aimed to investigate the role of CPT1A, which is a succinyltransferase in the progression of prostate cancer (PCa). CCK-8 was used to detect the cell viability. Seahorse was performed to evaluate the cell glyc...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2024-12-01
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| Series: | Cancer Biology & Therapy |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/15384047.2024.2329372 |
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| author | Shufeng Liu Xiaoguang Chen Liqi Zhang Bo Lu |
| author_facet | Shufeng Liu Xiaoguang Chen Liqi Zhang Bo Lu |
| author_sort | Shufeng Liu |
| collection | DOAJ |
| description | Succinylation modification involves in the progression of human cancers. The present study aimed to investigate the role of CPT1A, which is a succinyltransferase in the progression of prostate cancer (PCa). CCK-8 was used to detect the cell viability. Seahorse was performed to evaluate the cell glycolysis. Luciferase assay was used to detect the transcriptional regulation. ChIP was performed to assess the binding between transcriptional factors with the promoters. Co-IP was used to assess the binding between proteins. We found that CPT1A was highly expressed in PCa tissues and cell lines. Silencing of CPT1A inhibited the viability and glycolysis of PCa cells. Mechanistically, CPT1A promoted the succinylation of SP5, which strengthened the binding between SP5 and the promoter of PDPK1. SP5 activated PDPK1 transcription and PDPK1 activated the AKT/mTOR signal pathway. These findings might provide novel targets for the diagnosis or therapy of prostate cancer. |
| format | Article |
| id | doaj-art-cb5ecdfdd8e545689f50e93157a10ffb |
| institution | OA Journals |
| issn | 1538-4047 1555-8576 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Cancer Biology & Therapy |
| spelling | doaj-art-cb5ecdfdd8e545689f50e93157a10ffb2025-08-20T01:58:48ZengTaylor & Francis GroupCancer Biology & Therapy1538-40471555-85762024-12-0125110.1080/15384047.2024.2329372CPT1A mediates the succinylation of SP5 which activates transcription of PDPK1 to promote the viability and glycolysis of prostate cancer cellsShufeng Liu0Xiaoguang Chen1Liqi Zhang2Bo Lu3Medical Department, Xiangyang Integrated Traditional and Western Medicine Hospital, Xiangyang, Hubei, ChinaUrology Department, Xiangyang Integrated Traditional and Western Medicine Hospital, Xiangyang, Hubei, ChinaLaboratory Department, Xiangyang Integrated Traditional and Western Medicine Hospital, Xiangyang, Hubei, ChinaLaboratory Department, Xiangyang Integrated Traditional and Western Medicine Hospital, Xiangyang, Hubei, ChinaSuccinylation modification involves in the progression of human cancers. The present study aimed to investigate the role of CPT1A, which is a succinyltransferase in the progression of prostate cancer (PCa). CCK-8 was used to detect the cell viability. Seahorse was performed to evaluate the cell glycolysis. Luciferase assay was used to detect the transcriptional regulation. ChIP was performed to assess the binding between transcriptional factors with the promoters. Co-IP was used to assess the binding between proteins. We found that CPT1A was highly expressed in PCa tissues and cell lines. Silencing of CPT1A inhibited the viability and glycolysis of PCa cells. Mechanistically, CPT1A promoted the succinylation of SP5, which strengthened the binding between SP5 and the promoter of PDPK1. SP5 activated PDPK1 transcription and PDPK1 activated the AKT/mTOR signal pathway. These findings might provide novel targets for the diagnosis or therapy of prostate cancer.https://www.tandfonline.com/doi/10.1080/15384047.2024.2329372Prostate cancerCPT1ASP5PDPK1succinylationtranscription |
| spellingShingle | Shufeng Liu Xiaoguang Chen Liqi Zhang Bo Lu CPT1A mediates the succinylation of SP5 which activates transcription of PDPK1 to promote the viability and glycolysis of prostate cancer cells Cancer Biology & Therapy Prostate cancer CPT1A SP5 PDPK1 succinylation transcription |
| title | CPT1A mediates the succinylation of SP5 which activates transcription of PDPK1 to promote the viability and glycolysis of prostate cancer cells |
| title_full | CPT1A mediates the succinylation of SP5 which activates transcription of PDPK1 to promote the viability and glycolysis of prostate cancer cells |
| title_fullStr | CPT1A mediates the succinylation of SP5 which activates transcription of PDPK1 to promote the viability and glycolysis of prostate cancer cells |
| title_full_unstemmed | CPT1A mediates the succinylation of SP5 which activates transcription of PDPK1 to promote the viability and glycolysis of prostate cancer cells |
| title_short | CPT1A mediates the succinylation of SP5 which activates transcription of PDPK1 to promote the viability and glycolysis of prostate cancer cells |
| title_sort | cpt1a mediates the succinylation of sp5 which activates transcription of pdpk1 to promote the viability and glycolysis of prostate cancer cells |
| topic | Prostate cancer CPT1A SP5 PDPK1 succinylation transcription |
| url | https://www.tandfonline.com/doi/10.1080/15384047.2024.2329372 |
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