In situ sex identification in senegalese sole (Solea senegalensis) using loop-mediated isothermal amplification
The Senegalese sole (Solea senegalensis) is a promising candidate for aquaculture diversification in southern Europe due to its favorable traits and high market value. However, reproductive challenges and sexual dimorphism in growth necessitate efficient sex identification to optimize male-to-female...
Saved in:
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2025-09-01
|
| Series: | Aquaculture Reports |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352513425003576 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849392503309991936 |
|---|---|
| author | Emma Nicole Short Laureana Rebordinos Alejandro Centeno-Cuadros |
| author_facet | Emma Nicole Short Laureana Rebordinos Alejandro Centeno-Cuadros |
| author_sort | Emma Nicole Short |
| collection | DOAJ |
| description | The Senegalese sole (Solea senegalensis) is a promising candidate for aquaculture diversification in southern Europe due to its favorable traits and high market value. However, reproductive challenges and sexual dimorphism in growth necessitate efficient sex identification to optimize male-to-female ratios in farmed populations. Current sexing techniques are often invasive and time-consuming, prompting a need for non-invasive, reliable alternatives. To this end, this study presents the first loop-mediated isothermal amplification (LAMP) protocol for molecular sex identification in Senegalese sole. We designed novel primers targeting sex-linked SNPs in the fshr gene for both LAMP and PCR-based assays and extracted DNA from fresh tissue and non-invasively from skin mucus. An open-tube LAMP method was optimized for laboratory use, followed by a closed-tube LAMP method for in situ applications, incorporating the dye eriochrome black T to minimize contamination risks. Both LAMP approaches successfully identified male DNA with high specificity, demonstrating full concordance with PCR results. This innovative sexing technique for Senegalese sole enabled rapid, non-invasive sexing directly from mucus samples, making it suitable for applications outside of the modern laboratory environment, in both aquaculture facilities and natural environments. |
| format | Article |
| id | doaj-art-cb5e45d722754f37a16d47adec5c7e6c |
| institution | Kabale University |
| issn | 2352-5134 |
| language | English |
| publishDate | 2025-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Aquaculture Reports |
| spelling | doaj-art-cb5e45d722754f37a16d47adec5c7e6c2025-08-20T03:40:46ZengElsevierAquaculture Reports2352-51342025-09-014310297110.1016/j.aqrep.2025.102971In situ sex identification in senegalese sole (Solea senegalensis) using loop-mediated isothermal amplificationEmma Nicole Short0Laureana Rebordinos1Alejandro Centeno-Cuadros2Genetics Laboratory, Faculty of Marine and Environmental Sciences. University of Cádiz. C/ República Árabe Saharaui, s/n. 11510, Puerto Real, Cádiz, SpainGenetics Laboratory, Faculty of Marine and Environmental Sciences. University of Cádiz. C/ República Árabe Saharaui, s/n. 11510, Puerto Real, Cádiz, Spain; Instituto de Investigaciones Marinas (INMAR), Puerto Real, Cádiz, SpainGenetics Laboratory, Faculty of Marine and Environmental Sciences. University of Cádiz. C/ República Árabe Saharaui, s/n. 11510, Puerto Real, Cádiz, Spain; Instituto de Investigaciones Marinas (INMAR), Puerto Real, Cádiz, Spain; Corresponding author at: Genetics Laboratory, Faculty of Marine and Environmental Sciences. University of Cádiz. C/ República Árabe Saharaui, s/n. 11510, Puerto Real, Cádiz, Spain.The Senegalese sole (Solea senegalensis) is a promising candidate for aquaculture diversification in southern Europe due to its favorable traits and high market value. However, reproductive challenges and sexual dimorphism in growth necessitate efficient sex identification to optimize male-to-female ratios in farmed populations. Current sexing techniques are often invasive and time-consuming, prompting a need for non-invasive, reliable alternatives. To this end, this study presents the first loop-mediated isothermal amplification (LAMP) protocol for molecular sex identification in Senegalese sole. We designed novel primers targeting sex-linked SNPs in the fshr gene for both LAMP and PCR-based assays and extracted DNA from fresh tissue and non-invasively from skin mucus. An open-tube LAMP method was optimized for laboratory use, followed by a closed-tube LAMP method for in situ applications, incorporating the dye eriochrome black T to minimize contamination risks. Both LAMP approaches successfully identified male DNA with high specificity, demonstrating full concordance with PCR results. This innovative sexing technique for Senegalese sole enabled rapid, non-invasive sexing directly from mucus samples, making it suitable for applications outside of the modern laboratory environment, in both aquaculture facilities and natural environments.http://www.sciencedirect.com/science/article/pii/S2352513425003576LAMPfshrMolecular sexingEriochrome black T, non-invasive sampling |
| spellingShingle | Emma Nicole Short Laureana Rebordinos Alejandro Centeno-Cuadros In situ sex identification in senegalese sole (Solea senegalensis) using loop-mediated isothermal amplification Aquaculture Reports LAMP fshr Molecular sexing Eriochrome black T, non-invasive sampling |
| title | In situ sex identification in senegalese sole (Solea senegalensis) using loop-mediated isothermal amplification |
| title_full | In situ sex identification in senegalese sole (Solea senegalensis) using loop-mediated isothermal amplification |
| title_fullStr | In situ sex identification in senegalese sole (Solea senegalensis) using loop-mediated isothermal amplification |
| title_full_unstemmed | In situ sex identification in senegalese sole (Solea senegalensis) using loop-mediated isothermal amplification |
| title_short | In situ sex identification in senegalese sole (Solea senegalensis) using loop-mediated isothermal amplification |
| title_sort | in situ sex identification in senegalese sole solea senegalensis using loop mediated isothermal amplification |
| topic | LAMP fshr Molecular sexing Eriochrome black T, non-invasive sampling |
| url | http://www.sciencedirect.com/science/article/pii/S2352513425003576 |
| work_keys_str_mv | AT emmanicoleshort insitusexidentificationinsenegalesesolesoleasenegalensisusingloopmediatedisothermalamplification AT laureanarebordinos insitusexidentificationinsenegalesesolesoleasenegalensisusingloopmediatedisothermalamplification AT alejandrocentenocuadros insitusexidentificationinsenegalesesolesoleasenegalensisusingloopmediatedisothermalamplification |