Organ In Vitro Culture: What Have We Learned about Early Kidney Development?

When Clifford Grobstein set out to study the inductive interaction between tissues in the developing embryo, he developed a method that remained important for the study of renal development until now. From the late 1950s on, in vitro cultivation of the metanephric kidney became a standard method. It...

Full description

Saved in:
Bibliographic Details
Main Authors: Aleksandra Rak-Raszewska, Peter V. Hauser, Seppo Vainio
Format: Article
Language:English
Published: Wiley 2015-01-01
Series:Stem Cells International
Online Access:http://dx.doi.org/10.1155/2015/959807
Tags: Add Tag
No Tags, Be the first to tag this record!
_version_ 1832557055125225472
author Aleksandra Rak-Raszewska
Peter V. Hauser
Seppo Vainio
author_facet Aleksandra Rak-Raszewska
Peter V. Hauser
Seppo Vainio
author_sort Aleksandra Rak-Raszewska
collection DOAJ
description When Clifford Grobstein set out to study the inductive interaction between tissues in the developing embryo, he developed a method that remained important for the study of renal development until now. From the late 1950s on, in vitro cultivation of the metanephric kidney became a standard method. It provided an artificial environment that served as an open platform to study organogenesis. This review provides an introduction to the technique of organ culture, describes how the Grobstein assay and its variants have been used to study aspects of mesenchymal induction, and describes the search for natural and chemical inducers of the metanephric mesenchyme. The review also focuses on renal development, starting with ectopic budding of the ureteric bud, ureteric bud branching, and the generation of the nephron and presents the search for stem cells and renal progenitor cells that contribute to specific structures and tissues during renal development. It also presents the current use of Grobstein assay and its modifications in regenerative medicine and tissue engineering today. Together, this review highlights the importance of ex vivo kidney studies as a way to acquire new knowledge, which in the future can and will be implemented for developmental biology and regenerative medicine applications.
format Article
id doaj-art-cb57f15d6edf4fd99b3f1322f7b5b688
institution Kabale University
issn 1687-966X
1687-9678
language English
publishDate 2015-01-01
publisher Wiley
record_format Article
series Stem Cells International
spelling doaj-art-cb57f15d6edf4fd99b3f1322f7b5b6882025-02-03T05:43:51ZengWileyStem Cells International1687-966X1687-96782015-01-01201510.1155/2015/959807959807Organ In Vitro Culture: What Have We Learned about Early Kidney Development?Aleksandra Rak-Raszewska0Peter V. Hauser1Seppo Vainio2Faculty of Biochemistry and Molecular Medicine, Biocenter Oulu, Oulu University, 90220 Oulu, FinlandRenal Regeneration Laboratory, VAGLAHS at Sepulveda, North Hills, CA 91343, USAFaculty of Biochemistry and Molecular Medicine, Biocenter Oulu, Oulu University, 90220 Oulu, FinlandWhen Clifford Grobstein set out to study the inductive interaction between tissues in the developing embryo, he developed a method that remained important for the study of renal development until now. From the late 1950s on, in vitro cultivation of the metanephric kidney became a standard method. It provided an artificial environment that served as an open platform to study organogenesis. This review provides an introduction to the technique of organ culture, describes how the Grobstein assay and its variants have been used to study aspects of mesenchymal induction, and describes the search for natural and chemical inducers of the metanephric mesenchyme. The review also focuses on renal development, starting with ectopic budding of the ureteric bud, ureteric bud branching, and the generation of the nephron and presents the search for stem cells and renal progenitor cells that contribute to specific structures and tissues during renal development. It also presents the current use of Grobstein assay and its modifications in regenerative medicine and tissue engineering today. Together, this review highlights the importance of ex vivo kidney studies as a way to acquire new knowledge, which in the future can and will be implemented for developmental biology and regenerative medicine applications.http://dx.doi.org/10.1155/2015/959807
spellingShingle Aleksandra Rak-Raszewska
Peter V. Hauser
Seppo Vainio
Organ In Vitro Culture: What Have We Learned about Early Kidney Development?
Stem Cells International
title Organ In Vitro Culture: What Have We Learned about Early Kidney Development?
title_full Organ In Vitro Culture: What Have We Learned about Early Kidney Development?
title_fullStr Organ In Vitro Culture: What Have We Learned about Early Kidney Development?
title_full_unstemmed Organ In Vitro Culture: What Have We Learned about Early Kidney Development?
title_short Organ In Vitro Culture: What Have We Learned about Early Kidney Development?
title_sort organ in vitro culture what have we learned about early kidney development
url http://dx.doi.org/10.1155/2015/959807
work_keys_str_mv AT aleksandrarakraszewska organinvitroculturewhathavewelearnedaboutearlykidneydevelopment
AT petervhauser organinvitroculturewhathavewelearnedaboutearlykidneydevelopment
AT seppovainio organinvitroculturewhathavewelearnedaboutearlykidneydevelopment