Exploring the recombinant evolution and hosts of crucivirus based on novel oyster-associated viruses
“Crucivirus” represents a group of viruses with chimeric genomes, significant for viral evolution and recombination studies. Their capsid proteins share homology with the RNA virus tombusvirus, while their replicase-associated proteins are homologous to a class of single-stranded DNA viruses, namely...
Saved in:
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2025-02-01
|
| Series: | Frontiers in Microbiology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2025.1454079/full |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832542335667273728 |
|---|---|
| author | Hong-Sai Zhang Hong-Sai Zhang Chang Liu Guang-Feng Liu Yu-Yu Chen Peng Zhu Peng Zhu Xin Xu Bing-Xin Yin Jing-Zhe Jiang Jing-Zhe Jiang |
| author_facet | Hong-Sai Zhang Hong-Sai Zhang Chang Liu Guang-Feng Liu Yu-Yu Chen Peng Zhu Peng Zhu Xin Xu Bing-Xin Yin Jing-Zhe Jiang Jing-Zhe Jiang |
| author_sort | Hong-Sai Zhang |
| collection | DOAJ |
| description | “Crucivirus” represents a group of viruses with chimeric genomes, significant for viral evolution and recombination studies. Their capsid proteins share homology with the RNA virus tombusvirus, while their replicase-associated proteins are homologous to a class of single-stranded DNA viruses, namely CRESS DNA viruses. This study identifies seven novel crucivirus genomes from oysters cultivated along the coast of the South China Sea. Phylogenetic analysis reveals that five sequences form a distinct branch, which may indicate the presence of a new subclass within the crucivirus family. We analyzed crucivirus from multiple perspectives, including viral genomes, hallmark proteins, sequence similarity, and potential hosts. The results indicate that the crucivirus genomes and replicase-associated proteins (Rep) from oysters conform to the typical characteristics of crucivirus; Crucivirus Rep appears to have a direct parallel origin from multiple clades of CRESS DNA viruses, while only the S-domain of their capsid proteins shows some evolutionary relationship with tombusvirus. We found protein sequences in rotifers that are highly similar to the Cap three-dimensional structure of crucivirus, which may suggest host relevance. Overall, this study provides new insights into the classification, evolution, and host origins of crucivirus. |
| format | Article |
| id | doaj-art-cb4e4d16ff9644e48f4fc54699051559 |
| institution | Kabale University |
| issn | 1664-302X |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj-art-cb4e4d16ff9644e48f4fc546990515592025-02-04T06:32:06ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2025-02-011610.3389/fmicb.2025.14540791454079Exploring the recombinant evolution and hosts of crucivirus based on novel oyster-associated virusesHong-Sai Zhang0Hong-Sai Zhang1Chang Liu2Guang-Feng Liu3Yu-Yu Chen4Peng Zhu5Peng Zhu6Xin Xu7Bing-Xin Yin8Jing-Zhe Jiang9Jing-Zhe Jiang10College of Fisheries and Life Science, Shanghai Ocean University, Shanghai, ChinaKey Laboratory of South China Sea Fishery Resources Exploitation and Utilization, Ministry of Agriculture and Rural Affairs, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou, ChinaKey Laboratory of South China Sea Fishery Resources Exploitation and Utilization, Ministry of Agriculture and Rural Affairs, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou, ChinaKey Laboratory of South China Sea Fishery Resources Exploitation and Utilization, Ministry of Agriculture and Rural Affairs, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou, ChinaKey Laboratory of South China Sea Fishery Resources Exploitation and Utilization, Ministry of Agriculture and Rural Affairs, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou, ChinaCollege of Fisheries and Life Science, Shanghai Ocean University, Shanghai, ChinaKey Laboratory of South China Sea Fishery Resources Exploitation and Utilization, Ministry of Agriculture and Rural Affairs, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou, ChinaLivestock, Aquaculture and Technology Promotion and Service Center of Conghua District, Guangzhou, ChinaKey Laboratory of South China Sea Fishery Resources Exploitation and Utilization, Ministry of Agriculture and Rural Affairs, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou, ChinaCollege of Fisheries and Life Science, Shanghai Ocean University, Shanghai, ChinaKey Laboratory of South China Sea Fishery Resources Exploitation and Utilization, Ministry of Agriculture and Rural Affairs, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou, China“Crucivirus” represents a group of viruses with chimeric genomes, significant for viral evolution and recombination studies. Their capsid proteins share homology with the RNA virus tombusvirus, while their replicase-associated proteins are homologous to a class of single-stranded DNA viruses, namely CRESS DNA viruses. This study identifies seven novel crucivirus genomes from oysters cultivated along the coast of the South China Sea. Phylogenetic analysis reveals that five sequences form a distinct branch, which may indicate the presence of a new subclass within the crucivirus family. We analyzed crucivirus from multiple perspectives, including viral genomes, hallmark proteins, sequence similarity, and potential hosts. The results indicate that the crucivirus genomes and replicase-associated proteins (Rep) from oysters conform to the typical characteristics of crucivirus; Crucivirus Rep appears to have a direct parallel origin from multiple clades of CRESS DNA viruses, while only the S-domain of their capsid proteins shows some evolutionary relationship with tombusvirus. We found protein sequences in rotifers that are highly similar to the Cap three-dimensional structure of crucivirus, which may suggest host relevance. Overall, this study provides new insights into the classification, evolution, and host origins of crucivirus.https://www.frontiersin.org/articles/10.3389/fmicb.2025.1454079/fullcrucivirusCRESS DNA virusesvirus evolutionhostgenomeoyster |
| spellingShingle | Hong-Sai Zhang Hong-Sai Zhang Chang Liu Guang-Feng Liu Yu-Yu Chen Peng Zhu Peng Zhu Xin Xu Bing-Xin Yin Jing-Zhe Jiang Jing-Zhe Jiang Exploring the recombinant evolution and hosts of crucivirus based on novel oyster-associated viruses Frontiers in Microbiology crucivirus CRESS DNA viruses virus evolution host genome oyster |
| title | Exploring the recombinant evolution and hosts of crucivirus based on novel oyster-associated viruses |
| title_full | Exploring the recombinant evolution and hosts of crucivirus based on novel oyster-associated viruses |
| title_fullStr | Exploring the recombinant evolution and hosts of crucivirus based on novel oyster-associated viruses |
| title_full_unstemmed | Exploring the recombinant evolution and hosts of crucivirus based on novel oyster-associated viruses |
| title_short | Exploring the recombinant evolution and hosts of crucivirus based on novel oyster-associated viruses |
| title_sort | exploring the recombinant evolution and hosts of crucivirus based on novel oyster associated viruses |
| topic | crucivirus CRESS DNA viruses virus evolution host genome oyster |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2025.1454079/full |
| work_keys_str_mv | AT hongsaizhang exploringtherecombinantevolutionandhostsofcrucivirusbasedonnoveloysterassociatedviruses AT hongsaizhang exploringtherecombinantevolutionandhostsofcrucivirusbasedonnoveloysterassociatedviruses AT changliu exploringtherecombinantevolutionandhostsofcrucivirusbasedonnoveloysterassociatedviruses AT guangfengliu exploringtherecombinantevolutionandhostsofcrucivirusbasedonnoveloysterassociatedviruses AT yuyuchen exploringtherecombinantevolutionandhostsofcrucivirusbasedonnoveloysterassociatedviruses AT pengzhu exploringtherecombinantevolutionandhostsofcrucivirusbasedonnoveloysterassociatedviruses AT pengzhu exploringtherecombinantevolutionandhostsofcrucivirusbasedonnoveloysterassociatedviruses AT xinxu exploringtherecombinantevolutionandhostsofcrucivirusbasedonnoveloysterassociatedviruses AT bingxinyin exploringtherecombinantevolutionandhostsofcrucivirusbasedonnoveloysterassociatedviruses AT jingzhejiang exploringtherecombinantevolutionandhostsofcrucivirusbasedonnoveloysterassociatedviruses AT jingzhejiang exploringtherecombinantevolutionandhostsofcrucivirusbasedonnoveloysterassociatedviruses |