Antiplatelet and antithrombotic effect of Phyllostachys pubescens leaves and Mume Fructus combination
Background: Agents currently used for the treatment and prevention of thrombosis have a number of side effects. We conducted this study to develop antithrombotic agents from herbs that are used in food. Methods: The 80% (v/v) ethanol extracts of Phyllostachys pubescens leaf (PL) and Mume Fructus (MF...
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| Format: | Article |
| Language: | English |
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Elsevier
2013-06-01
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| Series: | Integrative Medicine Research |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213422013000243 |
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| author | Wen Yi Jin Seung-Hyung Kim Ho Kyoung Kim Dong Gyu Jang Jeong Bum Nam Young Min Kang Bang Yeon Hwang Dong-Seon Kim |
| author_facet | Wen Yi Jin Seung-Hyung Kim Ho Kyoung Kim Dong Gyu Jang Jeong Bum Nam Young Min Kang Bang Yeon Hwang Dong-Seon Kim |
| author_sort | Wen Yi Jin |
| collection | DOAJ |
| description | Background: Agents currently used for the treatment and prevention of thrombosis have a number of side effects. We conducted this study to develop antithrombotic agents from herbs that are used in food.
Methods: The 80% (v/v) ethanol extracts of Phyllostachys pubescens leaf (PL) and Mume Fructus (MF) and their combinations—2:1 (PM21), 1:1 (PM11), and 1:2 (PM12)—were evaluated on rat platelet aggregation induced by adenosine diphosphate (ADP) in vitro and on arteriovenous shunt thrombosis after 3 days of oral treatment in rats in vivo.
Results: At 100 μg/mL, PM21 and PM11 inhibited in vitro ADP-induced aggregation by 44.0 ± 4.3% and 30.0 ± 3.2%, respectively, whereas PL, MF, and PM12 weakly or scarcely inhibited ADP-induced aggregation by 3.9 ± 3.2%, 13.0 ± 2.7%, and 5.2 ± 1.3%, respectively. The IC50 values of PM21 on ADP-, collagen-, and thrombin-induced platelet aggregations were 135.6 ± 7.4 μg/mL, 142.7 ± 5.8 μg/mL, and 186.5 ± 9.7 μg/mL, respectively. In an in vivo rat arteriovenous-shunt thrombosis model, thrombus weight was significantly decreased after the oral administration of 400 mg/kg PL (27.8 ± 3.0%, p < 0.01) or MF (35.2 ± 2.1%, p < 0.01), and with a good accord to the in vitro results, the combination of PL and MF in the ratio of 2:1, PM21 (60.9 ± 1.2%, p < 0.001), showed a superior antithrombotic effect to those of individual extracts. At dosages of 200 mg/kg, 100 mg/kg, and 50 mg/kg, PM21 dose-dependently decreased thrombosis weight (ED50, 314 mg/kg).
Conclusion: These results suggest that combination preparations of PL and MF, especially their 2:1 combination, can increase antiplatelet and antithromboticeffects more than PL and MF alone, offering evidence for a potential novel combination antithrombotic therapy. |
| format | Article |
| id | doaj-art-cb2b2e180f8d4c10981afa5f0bb514f2 |
| institution | DOAJ |
| issn | 2213-4220 |
| language | English |
| publishDate | 2013-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Integrative Medicine Research |
| spelling | doaj-art-cb2b2e180f8d4c10981afa5f0bb514f22025-08-20T03:04:46ZengElsevierIntegrative Medicine Research2213-42202013-06-0122707510.1016/j.imr.2013.04.005Antiplatelet and antithrombotic effect of Phyllostachys pubescens leaves and Mume Fructus combinationWen Yi Jin0Seung-Hyung Kim1Ho Kyoung Kim2Dong Gyu Jang3Jeong Bum Nam4Young Min Kang5Bang Yeon Hwang6Dong-Seon Kim7KOC Biotech Inc., Daejeon 306-791, South KoreaInstitute of Traditional Medicine and Bioscience, Daejeon University, Daejeon 300-716, South KoreaBasic Herbal Medicine Research Group, Korea Institute of Oriental Medicine, Daejeon 305-811, South KoreaKOC Biotech Inc., Daejeon 306-791, South KoreaCollege of Pharmacy, Chungbuk National University, Cheongju 361-763, South KoreaBasic Herbal Medicine Research Group, Korea Institute of Oriental Medicine, Daejeon 305-811, South KoreaCollege of Pharmacy, Chungbuk National University, Cheongju 361-763, South KoreaBasic Herbal Medicine Research Group, Korea Institute of Oriental Medicine, Daejeon 305-811, South KoreaBackground: Agents currently used for the treatment and prevention of thrombosis have a number of side effects. We conducted this study to develop antithrombotic agents from herbs that are used in food. Methods: The 80% (v/v) ethanol extracts of Phyllostachys pubescens leaf (PL) and Mume Fructus (MF) and their combinations—2:1 (PM21), 1:1 (PM11), and 1:2 (PM12)—were evaluated on rat platelet aggregation induced by adenosine diphosphate (ADP) in vitro and on arteriovenous shunt thrombosis after 3 days of oral treatment in rats in vivo. Results: At 100 μg/mL, PM21 and PM11 inhibited in vitro ADP-induced aggregation by 44.0 ± 4.3% and 30.0 ± 3.2%, respectively, whereas PL, MF, and PM12 weakly or scarcely inhibited ADP-induced aggregation by 3.9 ± 3.2%, 13.0 ± 2.7%, and 5.2 ± 1.3%, respectively. The IC50 values of PM21 on ADP-, collagen-, and thrombin-induced platelet aggregations were 135.6 ± 7.4 μg/mL, 142.7 ± 5.8 μg/mL, and 186.5 ± 9.7 μg/mL, respectively. In an in vivo rat arteriovenous-shunt thrombosis model, thrombus weight was significantly decreased after the oral administration of 400 mg/kg PL (27.8 ± 3.0%, p < 0.01) or MF (35.2 ± 2.1%, p < 0.01), and with a good accord to the in vitro results, the combination of PL and MF in the ratio of 2:1, PM21 (60.9 ± 1.2%, p < 0.001), showed a superior antithrombotic effect to those of individual extracts. At dosages of 200 mg/kg, 100 mg/kg, and 50 mg/kg, PM21 dose-dependently decreased thrombosis weight (ED50, 314 mg/kg). Conclusion: These results suggest that combination preparations of PL and MF, especially their 2:1 combination, can increase antiplatelet and antithromboticeffects more than PL and MF alone, offering evidence for a potential novel combination antithrombotic therapy.http://www.sciencedirect.com/science/article/pii/S2213422013000243antiplateletantithrombosisblood circulationherbal combinationMume FructusPhyllostachys pubescens |
| spellingShingle | Wen Yi Jin Seung-Hyung Kim Ho Kyoung Kim Dong Gyu Jang Jeong Bum Nam Young Min Kang Bang Yeon Hwang Dong-Seon Kim Antiplatelet and antithrombotic effect of Phyllostachys pubescens leaves and Mume Fructus combination Integrative Medicine Research antiplatelet antithrombosis blood circulation herbal combination Mume Fructus Phyllostachys pubescens |
| title | Antiplatelet and antithrombotic effect of Phyllostachys pubescens leaves and Mume Fructus combination |
| title_full | Antiplatelet and antithrombotic effect of Phyllostachys pubescens leaves and Mume Fructus combination |
| title_fullStr | Antiplatelet and antithrombotic effect of Phyllostachys pubescens leaves and Mume Fructus combination |
| title_full_unstemmed | Antiplatelet and antithrombotic effect of Phyllostachys pubescens leaves and Mume Fructus combination |
| title_short | Antiplatelet and antithrombotic effect of Phyllostachys pubescens leaves and Mume Fructus combination |
| title_sort | antiplatelet and antithrombotic effect of phyllostachys pubescens leaves and mume fructus combination |
| topic | antiplatelet antithrombosis blood circulation herbal combination Mume Fructus Phyllostachys pubescens |
| url | http://www.sciencedirect.com/science/article/pii/S2213422013000243 |
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