Estimation of Indole-3-Acetic Acid Production from New Molecularly Characterized Rhizobacterial Strains
From different fields in Mosul city, we collected sixteen samples of soil surrounding the rhizosphere zone to isolate and characterize rhizobacterial species based on biochemical tests, 16S rRNA gene primer amplification using PCR and nucleotide sequence analysis, and similarity to global isola...
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| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Society for Innovative Agriculture
2025-01-01
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| Series: | Journal of Global Innovations in Agricultural Sciences |
| Online Access: | https://jgiass.com/pdf-reader.php?file=Estimation-of-Indole-3-Acetic-Acid-Production-from-New-Molecularly-Characterized-Rhizobacterial-Strains.pdf&path=issue_papers |
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| Summary: | From different fields in Mosul city, we collected sixteen samples of soil surrounding the rhizosphere zone to isolate and characterize rhizobacterial species based on biochemical tests, 16S rRNA gene primer amplification using PCR and nucleotide sequence analysis, and similarity to global isolates in the gene bank. All isolates were bacilliform, with clear differences in their response to Gram staining and biochemical tests. On the other hand, the results showed that root colonizing bacteria could produce different amounts of indole-3-acetic acid (IAA). Molecular analysis tests based on the 16S rRNA primer gene were carried out to characterize the isolated bacteria at the molecular level and showed 99% homology with Azotobacter tropicalis SC39, Azotobacter chroococcum A11, Bacillus subtilis N22 and Ralstonia pickettii ULM005, which are registered worldwide in GenBank. It should be noted that in the diagnostic isolate R. pickettii both A and T were deleted, G was replaced by C and T was added at position 508. On the other hand, in the B. subtilis isolate, the nitrogenous bases A, G and G were deleted and the nitrogenous base G was replaced by the base C. The data for the third isolate, A. tropicalis, showed deletion of the nitrogenous bases C and T and replacement of G by A and A by T. In the fourth isolate, A. chroococcum, deletion of the nitrogenous base, replacement of C by A and C by T and addition of G, T and A in three positions were observed. These will be used as the basis for future scientific experiments to develop new biofertilizers from the rhizobacteria studied for the production of environmentally sustainable crops. |
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| ISSN: | 2788-4538 2788-4546 |