Differential effects of hypoxia on motility using various in vitro models of lung adenocarcinoma
Abstract Lung cancer is the leading cause of cancer-related death globally. Metastasis is the most common reason of mortality in which hypoxia is suggested to have a pivotal role. However, the effect of hypoxia on the metastatic potential and migratory activity of cancer cells is largely unexplored...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2024-09-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-024-70769-w |
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| author | Sára Eszter Surguta Marcell Baranyi Laura Svajda Mihály Cserepes Ivan Ranđelović Enikő Tátrai Balázs Hegedűs József Tóvári |
| author_facet | Sára Eszter Surguta Marcell Baranyi Laura Svajda Mihály Cserepes Ivan Ranđelović Enikő Tátrai Balázs Hegedűs József Tóvári |
| author_sort | Sára Eszter Surguta |
| collection | DOAJ |
| description | Abstract Lung cancer is the leading cause of cancer-related death globally. Metastasis is the most common reason of mortality in which hypoxia is suggested to have a pivotal role. However, the effect of hypoxia on the metastatic potential and migratory activity of cancer cells is largely unexplored and warrants detailed scientific investigations. Accordingly, we analyzed changes on cell proliferation and migratory activity both in single-cell migration and invasion under normoxic and hypoxic conditions in lung adenocarcinoma cell lines. Alterations in crucial genes and proteins associated with cellular response to hypoxia, epithelial-mesenchymal transition, proliferation and apoptosis were also analyzed. Generally, we observed no change in proliferation upon hypoxic conditions and no detectable induction of apoptosis. Interestingly, we observed that single-cell motility was generally reduced while invasion under confluent conditions using scratch assay was enhanced by hypoxia in most of the cell lines. Furthermore, we detected changes in the expression of EMT markers that are consistent with enhanced motility and metastasis-promoting effect of hypoxia. In summary, our study indicated cell line-, time of exposure- and migrational type-dependent effects of hypoxia in cellular proliferation, motility and gene expression. Our results contribute to better understanding and tackling cancer metastasis. |
| format | Article |
| id | doaj-art-cb277a475cdd4d908f18552eefd4c861 |
| institution | DOAJ |
| issn | 2045-2322 |
| language | English |
| publishDate | 2024-09-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj-art-cb277a475cdd4d908f18552eefd4c8612025-08-20T02:39:40ZengNature PortfolioScientific Reports2045-23222024-09-0114111210.1038/s41598-024-70769-wDifferential effects of hypoxia on motility using various in vitro models of lung adenocarcinomaSára Eszter Surguta0Marcell Baranyi1Laura Svajda2Mihály Cserepes3Ivan Ranđelović4Enikő Tátrai5Balázs Hegedűs6József Tóvári7Department of Experimental Pharmacology and the National Tumor Biology Laboratory, National Institute of OncologyDepartment of Pathology, Forensic and Insurance Medicine, Semmelweis UniversityDepartment of Experimental Pharmacology and the National Tumor Biology Laboratory, National Institute of OncologyDepartment of Experimental Pharmacology and the National Tumor Biology Laboratory, National Institute of OncologyDepartment of Experimental Pharmacology and the National Tumor Biology Laboratory, National Institute of OncologyDepartment of Experimental Pharmacology and the National Tumor Biology Laboratory, National Institute of OncologyDepartment of Pathology, Forensic and Insurance Medicine, Semmelweis UniversityDepartment of Experimental Pharmacology and the National Tumor Biology Laboratory, National Institute of OncologyAbstract Lung cancer is the leading cause of cancer-related death globally. Metastasis is the most common reason of mortality in which hypoxia is suggested to have a pivotal role. However, the effect of hypoxia on the metastatic potential and migratory activity of cancer cells is largely unexplored and warrants detailed scientific investigations. Accordingly, we analyzed changes on cell proliferation and migratory activity both in single-cell migration and invasion under normoxic and hypoxic conditions in lung adenocarcinoma cell lines. Alterations in crucial genes and proteins associated with cellular response to hypoxia, epithelial-mesenchymal transition, proliferation and apoptosis were also analyzed. Generally, we observed no change in proliferation upon hypoxic conditions and no detectable induction of apoptosis. Interestingly, we observed that single-cell motility was generally reduced while invasion under confluent conditions using scratch assay was enhanced by hypoxia in most of the cell lines. Furthermore, we detected changes in the expression of EMT markers that are consistent with enhanced motility and metastasis-promoting effect of hypoxia. In summary, our study indicated cell line-, time of exposure- and migrational type-dependent effects of hypoxia in cellular proliferation, motility and gene expression. Our results contribute to better understanding and tackling cancer metastasis.https://doi.org/10.1038/s41598-024-70769-w |
| spellingShingle | Sára Eszter Surguta Marcell Baranyi Laura Svajda Mihály Cserepes Ivan Ranđelović Enikő Tátrai Balázs Hegedűs József Tóvári Differential effects of hypoxia on motility using various in vitro models of lung adenocarcinoma Scientific Reports |
| title | Differential effects of hypoxia on motility using various in vitro models of lung adenocarcinoma |
| title_full | Differential effects of hypoxia on motility using various in vitro models of lung adenocarcinoma |
| title_fullStr | Differential effects of hypoxia on motility using various in vitro models of lung adenocarcinoma |
| title_full_unstemmed | Differential effects of hypoxia on motility using various in vitro models of lung adenocarcinoma |
| title_short | Differential effects of hypoxia on motility using various in vitro models of lung adenocarcinoma |
| title_sort | differential effects of hypoxia on motility using various in vitro models of lung adenocarcinoma |
| url | https://doi.org/10.1038/s41598-024-70769-w |
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