Molecular and biological properties of the Newcastle disease virus strain of virulent genotype VII, first isolated in 2008 from a wild duck in the Republic of Adygea, Russia

Aim. To study some biological properties of the obtained strain of Newcastle disease virus NDV/Adygea/duck/12/2008, including the degree of virulence, and to conduct a phylogenetic study.Biological material from wild migratory birds was collected in 2008 during the hunting season. Isolation and cult...

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Main Authors: A. V. Glushchenko, K. S. Yurchenko, L. S. Adamenko, Yu. I. Karkavin
Format: Article
Language:Russian
Published: Kamerton 2025-07-01
Series:Юг России: экология, развитие
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Online Access:https://ecodag.elpub.ru/ugro/article/view/3434
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author A. V. Glushchenko
K. S. Yurchenko
L. S. Adamenko
Yu. I. Karkavin
author_facet A. V. Glushchenko
K. S. Yurchenko
L. S. Adamenko
Yu. I. Karkavin
author_sort A. V. Glushchenko
collection DOAJ
description Aim. To study some biological properties of the obtained strain of Newcastle disease virus NDV/Adygea/duck/12/2008, including the degree of virulence, and to conduct a phylogenetic study.Biological material from wild migratory birds was collected in 2008 during the hunting season. Isolation and cultivation of the isolated strains were carried out in the system of developing chicken embryos (RCE). Primary identification confirming the presence of a hemagglutinating agent in the allantoic fluid was carried out in the hemagglutination reaction (HR). Pathogenicity was assessed by MDT and ICPI methods. Sequencing, phylogenetic analysis, the genotype of the studied strain was determined.The results of studying the main biological properties of the Newcastle disease virus strain NDV/Adygea/duck/12/2008, isolated from wild migratory birds in the Southern Federal District, are presented. According to the phylogenetic study, the NDV/Adygea/duck/12/2008 strain belongs to genotype VII and genetic class 2. The MDT and ICPI virulence tests, as well as the molecular genetic study, classified the described strain as highly pathogenic.
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series Юг России: экология, развитие
spelling doaj-art-cb23e46ffd714094b285bbd0255f039c2025-08-20T03:56:32ZrusKamertonЮг России: экология, развитие1992-10982413-09582025-07-0120261310.18470/1992-1098-2025-2-11448Molecular and biological properties of the Newcastle disease virus strain of virulent genotype VII, first isolated in 2008 from a wild duck in the Republic of Adygea, RussiaA. V. Glushchenko0K. S. Yurchenko1L. S. Adamenko2Yu. I. Karkavin3Federal Research Center of Fundamental and Translational Medicine, Siberian Branch, Russian Academy of SciencesFederal Research Center of Fundamental and Translational Medicine, Siberian Branch, Russian Academy of SciencesFederal Research Center of Fundamental and Translational Medicine, Siberian Branch, Russian Academy of SciencesFederal Research Center of Fundamental and Translational Medicine, Siberian Branch, Russian Academy of SciencesAim. To study some biological properties of the obtained strain of Newcastle disease virus NDV/Adygea/duck/12/2008, including the degree of virulence, and to conduct a phylogenetic study.Biological material from wild migratory birds was collected in 2008 during the hunting season. Isolation and cultivation of the isolated strains were carried out in the system of developing chicken embryos (RCE). Primary identification confirming the presence of a hemagglutinating agent in the allantoic fluid was carried out in the hemagglutination reaction (HR). Pathogenicity was assessed by MDT and ICPI methods. Sequencing, phylogenetic analysis, the genotype of the studied strain was determined.The results of studying the main biological properties of the Newcastle disease virus strain NDV/Adygea/duck/12/2008, isolated from wild migratory birds in the Southern Federal District, are presented. According to the phylogenetic study, the NDV/Adygea/duck/12/2008 strain belongs to genotype VII and genetic class 2. The MDT and ICPI virulence tests, as well as the molecular genetic study, classified the described strain as highly pathogenic.https://ecodag.elpub.ru/ugro/article/view/3434newcastle disease virusavian paramyxoviruseswild migratory birdsvelogenic straingenotype vii
spellingShingle A. V. Glushchenko
K. S. Yurchenko
L. S. Adamenko
Yu. I. Karkavin
Molecular and biological properties of the Newcastle disease virus strain of virulent genotype VII, first isolated in 2008 from a wild duck in the Republic of Adygea, Russia
Юг России: экология, развитие
newcastle disease virus
avian paramyxoviruses
wild migratory birds
velogenic strain
genotype vii
title Molecular and biological properties of the Newcastle disease virus strain of virulent genotype VII, first isolated in 2008 from a wild duck in the Republic of Adygea, Russia
title_full Molecular and biological properties of the Newcastle disease virus strain of virulent genotype VII, first isolated in 2008 from a wild duck in the Republic of Adygea, Russia
title_fullStr Molecular and biological properties of the Newcastle disease virus strain of virulent genotype VII, first isolated in 2008 from a wild duck in the Republic of Adygea, Russia
title_full_unstemmed Molecular and biological properties of the Newcastle disease virus strain of virulent genotype VII, first isolated in 2008 from a wild duck in the Republic of Adygea, Russia
title_short Molecular and biological properties of the Newcastle disease virus strain of virulent genotype VII, first isolated in 2008 from a wild duck in the Republic of Adygea, Russia
title_sort molecular and biological properties of the newcastle disease virus strain of virulent genotype vii first isolated in 2008 from a wild duck in the republic of adygea russia
topic newcastle disease virus
avian paramyxoviruses
wild migratory birds
velogenic strain
genotype vii
url https://ecodag.elpub.ru/ugro/article/view/3434
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