Assessment of cancer biomarkers in the Grenfell firefighter cohort study

Abstract Firefighters are exposed to a diverse range of harmful substances, including polycyclic aromatic hydrocarbons, benzene, and other carcinogens. These toxic compounds induce DNA damage, often causing the formation of DNA adducts and other lesions that can contribute to the development of vari...

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Main Authors: Johanna Feary, Yizhou Yu, Tamanna Kabir, Susie Schofield, Adrian Bevan, Victoria Askinyte, Katherine Honan, Liza Emirali, Andrea Rubbi, Anne E. Willis, Paul Cullinan, Shubha Anand, L. Miguel Martins
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Language:English
Published: Nature Portfolio 2025-05-01
Series:Scientific Reports
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Online Access:https://doi.org/10.1038/s41598-025-95991-y
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author Johanna Feary
Yizhou Yu
Tamanna Kabir
Susie Schofield
Adrian Bevan
Victoria Askinyte
Katherine Honan
Liza Emirali
Andrea Rubbi
Anne E. Willis
Paul Cullinan
Shubha Anand
L. Miguel Martins
author_facet Johanna Feary
Yizhou Yu
Tamanna Kabir
Susie Schofield
Adrian Bevan
Victoria Askinyte
Katherine Honan
Liza Emirali
Andrea Rubbi
Anne E. Willis
Paul Cullinan
Shubha Anand
L. Miguel Martins
author_sort Johanna Feary
collection DOAJ
description Abstract Firefighters are exposed to a diverse range of harmful substances, including polycyclic aromatic hydrocarbons, benzene, and other carcinogens. These toxic compounds induce DNA damage, often causing the formation of DNA adducts and other lesions that can contribute to the development of various diseases, including cancer. Recent advancements in molecular diagnostics have shown that circulating cell-free DNA (cfDNA) in plasma is a valuable biomarker for detecting DNA damage and disease states. In this study, we explored whether changes in the quantity and quality of plasma cfDNA might reveal DNA lesions or serve as early markers for diseases such as cancer in UK firefighters. Whilst there are few published epidemiological studies of risk of cancer in UK firefighters, there are none on molecular markers in this population. All the 685 firefighters who participated in the study were employed by the London Fire Brigade in 2017; many of them also attended the Grenfell Tower fire, the most devastating fire to occur in the UK in modern history. In this exploratory analysis, we sought to gain insights into the potential long-term health impacts of toxic smoke exposure on these first responders by analysing both the concentration of cfDNA present and specific genetic alterations in cfDNA. Using next-generation sequencing and a panel that detects pathogenic DNA variants linked to various cancers, we analysed a subset of 261 firefighters. Our findings revealed that 11 firefighters carried pathogenic DNA variants associated with cancer, but we found no association between fire smoke exposure and the presence of these variants.
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spelling doaj-art-cb23bf34a6384fabb71e72f796ed9b262025-08-20T01:49:42ZengNature PortfolioScientific Reports2045-23222025-05-0115111210.1038/s41598-025-95991-yAssessment of cancer biomarkers in the Grenfell firefighter cohort studyJohanna Feary0Yizhou Yu1Tamanna Kabir2Susie Schofield3Adrian Bevan4Victoria Askinyte5Katherine Honan6Liza Emirali7Andrea Rubbi8Anne E. Willis9Paul CullinanShubha Anand10L. Miguel Martins11National Heart & Lung Institute, Imperial CollegeMRC Toxicology Unit, University of CambridgeRoyal Brompton Hospital, Guys and St Thomas’ NHS Foundation TrustNational Heart & Lung Institute, Imperial CollegeLondon Fire BrigadeCancer Molecular Diagnostics Laboratory, Department of Oncology, University of CambridgeCancer Molecular Diagnostics Laboratory, Department of Oncology, University of CambridgeMRC Toxicology Unit, University of CambridgeMRC Toxicology Unit, University of CambridgeMRC Toxicology Unit, University of CambridgeCancer Molecular Diagnostics Laboratory, Department of Oncology, University of CambridgeMRC Toxicology Unit, University of CambridgeAbstract Firefighters are exposed to a diverse range of harmful substances, including polycyclic aromatic hydrocarbons, benzene, and other carcinogens. These toxic compounds induce DNA damage, often causing the formation of DNA adducts and other lesions that can contribute to the development of various diseases, including cancer. Recent advancements in molecular diagnostics have shown that circulating cell-free DNA (cfDNA) in plasma is a valuable biomarker for detecting DNA damage and disease states. In this study, we explored whether changes in the quantity and quality of plasma cfDNA might reveal DNA lesions or serve as early markers for diseases such as cancer in UK firefighters. Whilst there are few published epidemiological studies of risk of cancer in UK firefighters, there are none on molecular markers in this population. All the 685 firefighters who participated in the study were employed by the London Fire Brigade in 2017; many of them also attended the Grenfell Tower fire, the most devastating fire to occur in the UK in modern history. In this exploratory analysis, we sought to gain insights into the potential long-term health impacts of toxic smoke exposure on these first responders by analysing both the concentration of cfDNA present and specific genetic alterations in cfDNA. Using next-generation sequencing and a panel that detects pathogenic DNA variants linked to various cancers, we analysed a subset of 261 firefighters. Our findings revealed that 11 firefighters carried pathogenic DNA variants associated with cancer, but we found no association between fire smoke exposure and the presence of these variants.https://doi.org/10.1038/s41598-025-95991-yFirefighterFire smokeCancerBiomarkers
spellingShingle Johanna Feary
Yizhou Yu
Tamanna Kabir
Susie Schofield
Adrian Bevan
Victoria Askinyte
Katherine Honan
Liza Emirali
Andrea Rubbi
Anne E. Willis
Paul Cullinan
Shubha Anand
L. Miguel Martins
Assessment of cancer biomarkers in the Grenfell firefighter cohort study
Scientific Reports
Firefighter
Fire smoke
Cancer
Biomarkers
title Assessment of cancer biomarkers in the Grenfell firefighter cohort study
title_full Assessment of cancer biomarkers in the Grenfell firefighter cohort study
title_fullStr Assessment of cancer biomarkers in the Grenfell firefighter cohort study
title_full_unstemmed Assessment of cancer biomarkers in the Grenfell firefighter cohort study
title_short Assessment of cancer biomarkers in the Grenfell firefighter cohort study
title_sort assessment of cancer biomarkers in the grenfell firefighter cohort study
topic Firefighter
Fire smoke
Cancer
Biomarkers
url https://doi.org/10.1038/s41598-025-95991-y
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