Heterogeneity of T cells regulates tumor immunity mediated by Helicobacter pylori infection in gastric cancer
Abstract The impact of Helicobacter pylori (H. pylori) status on gastric cancer survival remains unclear. In this study, we conducted a prognostic analysis of 488 gastric cancer patients and performed single-cell RNA sequencing (scRNA-seq) on 18,717 T cells from six tumor samples with varying H. pyl...
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BMC
2025-03-01
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| Series: | BMC Cancer |
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| Online Access: | https://doi.org/10.1186/s12885-025-13957-9 |
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| author | Zhisheng Wu Xinya Wang Shujing Shi Deyuan Kong Chuanli Ren Lijun Bian Yuanliang Gu Fangmei An Qiang Zhan Caiwang Yan Chupeng Hu Yun Chen Runqiu Jiang Jinfei Chen |
| author_facet | Zhisheng Wu Xinya Wang Shujing Shi Deyuan Kong Chuanli Ren Lijun Bian Yuanliang Gu Fangmei An Qiang Zhan Caiwang Yan Chupeng Hu Yun Chen Runqiu Jiang Jinfei Chen |
| author_sort | Zhisheng Wu |
| collection | DOAJ |
| description | Abstract The impact of Helicobacter pylori (H. pylori) status on gastric cancer survival remains unclear. In this study, we conducted a prognostic analysis of 488 gastric cancer patients and performed single-cell RNA sequencing (scRNA-seq) on 18,717 T cells from six tumor samples with varying H. pylori statuses. Our findings revealed that gastric cancer patients with H. pylori infection had significantly longer survival times compared to those with negative H. pylori status. After unsupervised re-clustering of T cells based on scRNA-seq data, we identified ten CD4+ and twelve CD8+ clusters. Among them, four CD8+ T cell clusters exhibited distinct distributions based on H. pylori infection status. One cluster, marked by CXCL13, showed high levels of IFNG and GZMB in H. pylori-infected patients, while another cluster, which expressed immune suppression related genes like AREG and PTGER2, was predominantly comprised of cells from non-infected patients. High PTGER2 expression was significantly associated with worse prognosis in patients with high CD8 expression. These insights advance our understanding of H. pylori’s influence on T cell responses in gastric cancer, aiding in treatment and prognostic strategies. |
| format | Article |
| id | doaj-art-cb1acc6f4b2b4da5a40da1f743e5f508 |
| institution | OA Journals |
| issn | 1471-2407 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Cancer |
| spelling | doaj-art-cb1acc6f4b2b4da5a40da1f743e5f5082025-08-20T02:10:14ZengBMCBMC Cancer1471-24072025-03-0125111410.1186/s12885-025-13957-9Heterogeneity of T cells regulates tumor immunity mediated by Helicobacter pylori infection in gastric cancerZhisheng Wu0Xinya Wang1Shujing Shi2Deyuan Kong3Chuanli Ren4Lijun Bian5Yuanliang Gu6Fangmei An7Qiang Zhan8Caiwang Yan9Chupeng Hu10Yun Chen11Runqiu Jiang12Jinfei Chen13School of Chemistry and Chemical Engineering, Center of Interventional Radiology and Vascular Surgery, Nurturing Center of Jiangsu Province for State Laboratory of AI Imaging & Interventional Radiology, Department of Radiology, Medical School, Zhongda Hospital, Southeast UniversityWuxi People’s Hospital, Wuxi People’s Hospital of Nanjing Medical University, Wuxi Medical CenterDepartment of Rehabilitation, School of Sport and Health, Nanjing Sport InstituteDepartment of Immunology, Key Laboratory of Human Functional Genomics of Jiangsu Province, Nanjing Medical UniversityDepartment of Laboratory Medicine, Clinical Medical College of Yangzhou UniversityDepartment of Epidemiology, Center for Global Health, School of Public Health, Nanjing Medical UniversityDepartment of Epidemiology, Center for Global Health, School of Public Health, Nanjing Medical UniversityWuxi People’s Hospital, Wuxi People’s Hospital of Nanjing Medical University, Wuxi Medical CenterWuxi People’s Hospital, Wuxi People’s Hospital of Nanjing Medical University, Wuxi Medical CenterWuxi People’s Hospital, Wuxi People’s Hospital of Nanjing Medical University, Wuxi Medical CenterDepartment of Immunology, Key Laboratory of Human Functional Genomics of Jiangsu Province, Nanjing Medical UniversitySchool of Chemistry and Chemical Engineering, Center of Interventional Radiology and Vascular Surgery, Nurturing Center of Jiangsu Province for State Laboratory of AI Imaging & Interventional Radiology, Department of Radiology, Medical School, Zhongda Hospital, Southeast UniversityJiangsu Laboratory of Molecular Medicine, Medical School of Nanjing UniversityDepartment of Oncology, the First Affiliated Hospital of Wenzhou Medical UniversityAbstract The impact of Helicobacter pylori (H. pylori) status on gastric cancer survival remains unclear. In this study, we conducted a prognostic analysis of 488 gastric cancer patients and performed single-cell RNA sequencing (scRNA-seq) on 18,717 T cells from six tumor samples with varying H. pylori statuses. Our findings revealed that gastric cancer patients with H. pylori infection had significantly longer survival times compared to those with negative H. pylori status. After unsupervised re-clustering of T cells based on scRNA-seq data, we identified ten CD4+ and twelve CD8+ clusters. Among them, four CD8+ T cell clusters exhibited distinct distributions based on H. pylori infection status. One cluster, marked by CXCL13, showed high levels of IFNG and GZMB in H. pylori-infected patients, while another cluster, which expressed immune suppression related genes like AREG and PTGER2, was predominantly comprised of cells from non-infected patients. High PTGER2 expression was significantly associated with worse prognosis in patients with high CD8 expression. These insights advance our understanding of H. pylori’s influence on T cell responses in gastric cancer, aiding in treatment and prognostic strategies.https://doi.org/10.1186/s12885-025-13957-9Gastric cancerHelicobacter pyloriImmune microenvironmentSurvival |
| spellingShingle | Zhisheng Wu Xinya Wang Shujing Shi Deyuan Kong Chuanli Ren Lijun Bian Yuanliang Gu Fangmei An Qiang Zhan Caiwang Yan Chupeng Hu Yun Chen Runqiu Jiang Jinfei Chen Heterogeneity of T cells regulates tumor immunity mediated by Helicobacter pylori infection in gastric cancer BMC Cancer Gastric cancer Helicobacter pylori Immune microenvironment Survival |
| title | Heterogeneity of T cells regulates tumor immunity mediated by Helicobacter pylori infection in gastric cancer |
| title_full | Heterogeneity of T cells regulates tumor immunity mediated by Helicobacter pylori infection in gastric cancer |
| title_fullStr | Heterogeneity of T cells regulates tumor immunity mediated by Helicobacter pylori infection in gastric cancer |
| title_full_unstemmed | Heterogeneity of T cells regulates tumor immunity mediated by Helicobacter pylori infection in gastric cancer |
| title_short | Heterogeneity of T cells regulates tumor immunity mediated by Helicobacter pylori infection in gastric cancer |
| title_sort | heterogeneity of t cells regulates tumor immunity mediated by helicobacter pylori infection in gastric cancer |
| topic | Gastric cancer Helicobacter pylori Immune microenvironment Survival |
| url | https://doi.org/10.1186/s12885-025-13957-9 |
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