Comparative genomic analysis of a novel heat-tolerant and euryhaline strain of unicellular marine cyanobacterium Cyanobacterium sp. DS4 from a high-temperature lagoon
Abstract Background Cyanobacteria have diversified through their long evolutionary history and occupy a wide range of environments on Earth. To advance our understanding of their adaptation mechanisms in extreme environments, we performed stress tolerance characterizations, whole genome sequencing,...
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2025-05-01
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| Series: | BMC Microbiology |
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| Online Access: | https://doi.org/10.1186/s12866-025-03993-7 |
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| author | Ching-Nen Nathan Chen Keng-Min Lin Yu-Chen Lin Hsin-Ying Chang Tze Ching Yong Yi-Fang Chiu Chih-Horng Kuo Hsiu-An Chu |
| author_facet | Ching-Nen Nathan Chen Keng-Min Lin Yu-Chen Lin Hsin-Ying Chang Tze Ching Yong Yi-Fang Chiu Chih-Horng Kuo Hsiu-An Chu |
| author_sort | Ching-Nen Nathan Chen |
| collection | DOAJ |
| description | Abstract Background Cyanobacteria have diversified through their long evolutionary history and occupy a wide range of environments on Earth. To advance our understanding of their adaptation mechanisms in extreme environments, we performed stress tolerance characterizations, whole genome sequencing, and comparative genomic analyses of a novel heat-tolerant and euryhaline strain of the unicellular cyanobacterium Cyanobacterium sp. Dongsha4 (DS4). This strain was isolated from a lagoon on Dongsha Island in the South China Sea, a habitat with fluctuations in temperature, salinity, light intensity, and nutrient supply. Results DS4 cells can tolerate long-term high-temperature up to 50 ℃ and salinity from 0 to 6.6%, which is similar to the results previously obtained for Cyanobacterium aponinum. In contrast, most mesophilic cyanobacteria cannot survive under these extreme conditions. Based on the 16S rRNA gene phylogeny, DS4 is most closely related to Cyanobacterium sp. NBRC102756 isolated from Iwojima Island, Japan, and Cyanobacterium sp. MCCB114 isolated from Vypeen Island, India. For comparison with strains that have genomic information available, DS4 is most similar to Cyanobacterium aponinum strain PCC10605 (PCC10605), sharing 81.7% of the genomic segments and 92.9% average nucleotide identity (ANI). Gene content comparisons identified multiple distinct features of DS4. Unlike related strains, DS4 possesses the genes necessary for nitrogen fixation. Other notable genes include those involved in photosynthesis, central metabolisms, cyanobacterial starch metabolisms, stress tolerances, and biosynthesis of novel secondary metabolites. Conclusions These findings promote our understanding of the physiology, ecology, evolution, and stress tolerance mechanisms of cyanobacteria. The information is valuable for future functional studies and biotechnology applications of heat-tolerant and euryhaline marine cyanobacteria. |
| format | Article |
| id | doaj-art-cb19e9bd6a264942b06dc2138ba33b21 |
| institution | OA Journals |
| issn | 1471-2180 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | BMC |
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| series | BMC Microbiology |
| spelling | doaj-art-cb19e9bd6a264942b06dc2138ba33b212025-08-20T02:15:00ZengBMCBMC Microbiology1471-21802025-05-0125111410.1186/s12866-025-03993-7Comparative genomic analysis of a novel heat-tolerant and euryhaline strain of unicellular marine cyanobacterium Cyanobacterium sp. DS4 from a high-temperature lagoonChing-Nen Nathan Chen0Keng-Min Lin1Yu-Chen Lin2Hsin-Ying Chang3Tze Ching Yong4Yi-Fang Chiu5Chih-Horng Kuo6Hsiu-An Chu7Department of Oceanography, National Sun Yat-sen UniversityInstitute of Plant and Microbial Biology, Academia SinicaInstitute of Plant and Microbial Biology, Academia SinicaInstitute of Plant and Microbial Biology, Academia SinicaDepartment of Oceanography, National Sun Yat-sen UniversityInstitute of Plant and Microbial Biology, Academia SinicaInstitute of Plant and Microbial Biology, Academia SinicaInstitute of Plant and Microbial Biology, Academia SinicaAbstract Background Cyanobacteria have diversified through their long evolutionary history and occupy a wide range of environments on Earth. To advance our understanding of their adaptation mechanisms in extreme environments, we performed stress tolerance characterizations, whole genome sequencing, and comparative genomic analyses of a novel heat-tolerant and euryhaline strain of the unicellular cyanobacterium Cyanobacterium sp. Dongsha4 (DS4). This strain was isolated from a lagoon on Dongsha Island in the South China Sea, a habitat with fluctuations in temperature, salinity, light intensity, and nutrient supply. Results DS4 cells can tolerate long-term high-temperature up to 50 ℃ and salinity from 0 to 6.6%, which is similar to the results previously obtained for Cyanobacterium aponinum. In contrast, most mesophilic cyanobacteria cannot survive under these extreme conditions. Based on the 16S rRNA gene phylogeny, DS4 is most closely related to Cyanobacterium sp. NBRC102756 isolated from Iwojima Island, Japan, and Cyanobacterium sp. MCCB114 isolated from Vypeen Island, India. For comparison with strains that have genomic information available, DS4 is most similar to Cyanobacterium aponinum strain PCC10605 (PCC10605), sharing 81.7% of the genomic segments and 92.9% average nucleotide identity (ANI). Gene content comparisons identified multiple distinct features of DS4. Unlike related strains, DS4 possesses the genes necessary for nitrogen fixation. Other notable genes include those involved in photosynthesis, central metabolisms, cyanobacterial starch metabolisms, stress tolerances, and biosynthesis of novel secondary metabolites. Conclusions These findings promote our understanding of the physiology, ecology, evolution, and stress tolerance mechanisms of cyanobacteria. The information is valuable for future functional studies and biotechnology applications of heat-tolerant and euryhaline marine cyanobacteria.https://doi.org/10.1186/s12866-025-03993-7CyanobacteriumThermotoleranceGenomeComparative genomics |
| spellingShingle | Ching-Nen Nathan Chen Keng-Min Lin Yu-Chen Lin Hsin-Ying Chang Tze Ching Yong Yi-Fang Chiu Chih-Horng Kuo Hsiu-An Chu Comparative genomic analysis of a novel heat-tolerant and euryhaline strain of unicellular marine cyanobacterium Cyanobacterium sp. DS4 from a high-temperature lagoon BMC Microbiology Cyanobacterium Thermotolerance Genome Comparative genomics |
| title | Comparative genomic analysis of a novel heat-tolerant and euryhaline strain of unicellular marine cyanobacterium Cyanobacterium sp. DS4 from a high-temperature lagoon |
| title_full | Comparative genomic analysis of a novel heat-tolerant and euryhaline strain of unicellular marine cyanobacterium Cyanobacterium sp. DS4 from a high-temperature lagoon |
| title_fullStr | Comparative genomic analysis of a novel heat-tolerant and euryhaline strain of unicellular marine cyanobacterium Cyanobacterium sp. DS4 from a high-temperature lagoon |
| title_full_unstemmed | Comparative genomic analysis of a novel heat-tolerant and euryhaline strain of unicellular marine cyanobacterium Cyanobacterium sp. DS4 from a high-temperature lagoon |
| title_short | Comparative genomic analysis of a novel heat-tolerant and euryhaline strain of unicellular marine cyanobacterium Cyanobacterium sp. DS4 from a high-temperature lagoon |
| title_sort | comparative genomic analysis of a novel heat tolerant and euryhaline strain of unicellular marine cyanobacterium cyanobacterium sp ds4 from a high temperature lagoon |
| topic | Cyanobacterium Thermotolerance Genome Comparative genomics |
| url | https://doi.org/10.1186/s12866-025-03993-7 |
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