Validation of a particle gel immunoassay for Trypanosoma cruzi antibody detection using plasma samples collected with capillary tubes
Introduction: The Chagas disease particle gel immunoassay (PaGIA-Chagas) is a simple, fast and practical test used for the diagnosis of the chronic Chagas disease and is based on anti-Trypanosoma cruzi antibody detection in serum. This study aimed to validate the PaGIA-Chagas on plasma collected wi...
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The Journal of Infection in Developing Countries
2010-05-01
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| Series: | Journal of Infection in Developing Countries |
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| Online Access: | https://jidc.org/index.php/journal/article/view/784 |
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| author | Marlene MY Kawai Jose AP Salas Luiz AP Tavares Cor JF Fontes |
| author_facet | Marlene MY Kawai Jose AP Salas Luiz AP Tavares Cor JF Fontes |
| author_sort | Marlene MY Kawai |
| collection | DOAJ |
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Introduction: The Chagas disease particle gel immunoassay (PaGIA-Chagas) is a simple, fast and practical test used for the diagnosis of the chronic Chagas disease and is based on anti-Trypanosoma cruzi antibody detection in serum. This study aimed to validate the PaGIA-Chagas on plasma collected with capillary tubes.
Methodology: Serum samples from 74 T. cruzi-infected and 26 non-infected individuals were tested by conventional indirect immunofluorescence and PaGIA-Chagas. Later, plasma specimens collected with capillary tubes from these same individuals were tested by PaGIA-Chagas. Results from serum samples tested by IFA and PAGIA- Chagas were considered as the reference standard to determine the accuracy parameters of the particle gel tested on plasma samples. The inter-test agreement of T. cruzi antibody detection by PaGIA-Chagas on serum and plasma was calculated using Kappa index.
Results: The PaGIA-Chagas performed on plasma collected with capillary tubes had sensitivity and specificity of 99% and 100%, respectively. The crude agreement observed with the results of the PaGIA-Chagas on plasma and serum was 99% and the Kappa index was 0.975 (CI95%: 0.782 - 1.000).
Conclusion: PaGIA-Chagas for anti-T. cruzi detection on plasma collected with capillary tubes is accurate and might be indicated to reduce fieldwork time and materials in epidemiological screening of chronic Chagas disease.
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| format | Article |
| id | doaj-art-cb190959603849d5b2fa6daf435a0307 |
| institution | OA Journals |
| issn | 1972-2680 |
| language | English |
| publishDate | 2010-05-01 |
| publisher | The Journal of Infection in Developing Countries |
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| series | Journal of Infection in Developing Countries |
| spelling | doaj-art-cb190959603849d5b2fa6daf435a03072025-08-20T02:27:18ZengThe Journal of Infection in Developing CountriesJournal of Infection in Developing Countries1972-26802010-05-0140910.3855/jidc.784Validation of a particle gel immunoassay for Trypanosoma cruzi antibody detection using plasma samples collected with capillary tubesMarlene MY Kawai0Jose AP Salas1Luiz AP Tavares2Cor JF Fontes3Hospital Universitario Julio Muller, Federal University of Mato Grosso, Cuiaba, Mato GrossoHospital Universitario Julio Muller, Federal University of Mato Grosso, Cuiaba, Mato GrossoHospital Universitario Julio Muller, Federal University of Mato Grosso, Cuiaba, Mato GrossoHospital Universitario Julio Muller, Federal University of Mato Grosso, Cuiaba, Mato Grosso Introduction: The Chagas disease particle gel immunoassay (PaGIA-Chagas) is a simple, fast and practical test used for the diagnosis of the chronic Chagas disease and is based on anti-Trypanosoma cruzi antibody detection in serum. This study aimed to validate the PaGIA-Chagas on plasma collected with capillary tubes. Methodology: Serum samples from 74 T. cruzi-infected and 26 non-infected individuals were tested by conventional indirect immunofluorescence and PaGIA-Chagas. Later, plasma specimens collected with capillary tubes from these same individuals were tested by PaGIA-Chagas. Results from serum samples tested by IFA and PAGIA- Chagas were considered as the reference standard to determine the accuracy parameters of the particle gel tested on plasma samples. The inter-test agreement of T. cruzi antibody detection by PaGIA-Chagas on serum and plasma was calculated using Kappa index. Results: The PaGIA-Chagas performed on plasma collected with capillary tubes had sensitivity and specificity of 99% and 100%, respectively. The crude agreement observed with the results of the PaGIA-Chagas on plasma and serum was 99% and the Kappa index was 0.975 (CI95%: 0.782 - 1.000). Conclusion: PaGIA-Chagas for anti-T. cruzi detection on plasma collected with capillary tubes is accurate and might be indicated to reduce fieldwork time and materials in epidemiological screening of chronic Chagas disease. https://jidc.org/index.php/journal/article/view/784Chagas diseaseDiagnosisParticle gel immunoassayCapillary tube |
| spellingShingle | Marlene MY Kawai Jose AP Salas Luiz AP Tavares Cor JF Fontes Validation of a particle gel immunoassay for Trypanosoma cruzi antibody detection using plasma samples collected with capillary tubes Journal of Infection in Developing Countries Chagas disease Diagnosis Particle gel immunoassay Capillary tube |
| title | Validation of a particle gel immunoassay for Trypanosoma cruzi antibody detection using plasma samples collected with capillary tubes |
| title_full | Validation of a particle gel immunoassay for Trypanosoma cruzi antibody detection using plasma samples collected with capillary tubes |
| title_fullStr | Validation of a particle gel immunoassay for Trypanosoma cruzi antibody detection using plasma samples collected with capillary tubes |
| title_full_unstemmed | Validation of a particle gel immunoassay for Trypanosoma cruzi antibody detection using plasma samples collected with capillary tubes |
| title_short | Validation of a particle gel immunoassay for Trypanosoma cruzi antibody detection using plasma samples collected with capillary tubes |
| title_sort | validation of a particle gel immunoassay for trypanosoma cruzi antibody detection using plasma samples collected with capillary tubes |
| topic | Chagas disease Diagnosis Particle gel immunoassay Capillary tube |
| url | https://jidc.org/index.php/journal/article/view/784 |
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