Magnetically bioprinted anisotropic hydrogels promote BMSC osteogenic differentiation for bone defect repair
Bone tissue engineering utilizing magnetic anisotropic hydrogels (MAHs) loaded with bone marrow-derived stem cells (BMSCs) offers a promising strategy to enhance the regeneration of bone defects due to their mechanotransduction and osteoinductive properties. However, the application of MAHs as bioin...
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Elsevier
2025-06-01
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| Series: | Materials Today Bio |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2590006425004454 |
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| author | Rong Xu Hua Zhang Yang Luo Shiyi Pan Chi Zhang Xiaochuan Wu Guofeng Zhang Cuicui Su Dongdong Xia |
| author_facet | Rong Xu Hua Zhang Yang Luo Shiyi Pan Chi Zhang Xiaochuan Wu Guofeng Zhang Cuicui Su Dongdong Xia |
| author_sort | Rong Xu |
| collection | DOAJ |
| description | Bone tissue engineering utilizing magnetic anisotropic hydrogels (MAHs) loaded with bone marrow-derived stem cells (BMSCs) offers a promising strategy to enhance the regeneration of bone defects due to their mechanotransduction and osteoinductive properties. However, the application of MAHs as bioinks for creating personalized 3D scaffolds faces significant challenges. Bioprinting necessitates a rapid sol-gel transition to enable the ink to form stable structures, whereas anisotropic shaping requires the ink to remain in a sol state post-printing, allowing magnetic particles to assemble freely under magnetic induction. To overcome these challenges, we develop a biomimetic MAH that recapitulate the anisotropic structures of the bone using a continuous Liquid-in-Liquid bioprinting method combined with magnetic induction. The constructed MAHs feature uniformly aligned Fe3O4 microfibers embedded within the bioprinted hydrogel filaments. These Fe3O4 microfibers provide microscale geometric cues that promote the elongation and osteogenic bioactivity of BMSCs through biomechanical signaling pathways. The implantation of the MAHs loaded with BMSCs in a critical-sized cranial defect model effectively accelerates the healing of bone injuries by facilitating collagen matrix development and promoting neovascularization. This study introduces a novel approach in the development of MAHs and presents a promising candidate for applications in bone tissue engineering and repair. |
| format | Article |
| id | doaj-art-cb166a94ebbb4a90ac52c4c31582d2c9 |
| institution | OA Journals |
| issn | 2590-0064 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Materials Today Bio |
| spelling | doaj-art-cb166a94ebbb4a90ac52c4c31582d2c92025-08-20T02:26:09ZengElsevierMaterials Today Bio2590-00642025-06-013210188510.1016/j.mtbio.2025.101885Magnetically bioprinted anisotropic hydrogels promote BMSC osteogenic differentiation for bone defect repairRong Xu0Hua Zhang1Yang Luo2Shiyi Pan3Chi Zhang4Xiaochuan Wu5Guofeng Zhang6Cuicui Su7Dongdong Xia8Department of Orthopedics, The First Affiliated Hospital of Ningbo University, Ningbo, 315000, Zhejiang, China; Research Institute of Smart Medicine and Biological Engineering, Health Science Center, Ningbo University, Ningbo, 315211, Zhejiang, ChinaResearch Institute of Smart Medicine and Biological Engineering, Health Science Center, Ningbo University, Ningbo, 315211, Zhejiang, China; Corresponding author.Research Institute of Smart Medicine and Biological Engineering, Health Science Center, Ningbo University, Ningbo, 315211, Zhejiang, ChinaThe Second Affiliated Hospital of Anhui Medical University, Hefei, 230601, Anhui, ChinaDepartment of Orthopedics, The First Affiliated Hospital of Ningbo University, Ningbo, 315000, Zhejiang, ChinaDepartment of Orthopedics, The First Affiliated Hospital of Ningbo University, Ningbo, 315000, Zhejiang, ChinaDepartment of Orthopedics, The First Affiliated Hospital of Ningbo University, Ningbo, 315000, Zhejiang, ChinaResearch Institute of Smart Medicine and Biological Engineering, Health Science Center, Ningbo University, Ningbo, 315211, Zhejiang, China; Corresponding author.Department of Orthopedics, The First Affiliated Hospital of Ningbo University, Ningbo, 315000, Zhejiang, China; Corresponding author.Bone tissue engineering utilizing magnetic anisotropic hydrogels (MAHs) loaded with bone marrow-derived stem cells (BMSCs) offers a promising strategy to enhance the regeneration of bone defects due to their mechanotransduction and osteoinductive properties. However, the application of MAHs as bioinks for creating personalized 3D scaffolds faces significant challenges. Bioprinting necessitates a rapid sol-gel transition to enable the ink to form stable structures, whereas anisotropic shaping requires the ink to remain in a sol state post-printing, allowing magnetic particles to assemble freely under magnetic induction. To overcome these challenges, we develop a biomimetic MAH that recapitulate the anisotropic structures of the bone using a continuous Liquid-in-Liquid bioprinting method combined with magnetic induction. The constructed MAHs feature uniformly aligned Fe3O4 microfibers embedded within the bioprinted hydrogel filaments. These Fe3O4 microfibers provide microscale geometric cues that promote the elongation and osteogenic bioactivity of BMSCs through biomechanical signaling pathways. The implantation of the MAHs loaded with BMSCs in a critical-sized cranial defect model effectively accelerates the healing of bone injuries by facilitating collagen matrix development and promoting neovascularization. This study introduces a novel approach in the development of MAHs and presents a promising candidate for applications in bone tissue engineering and repair.http://www.sciencedirect.com/science/article/pii/S2590006425004454Anisotropic hydrogelBioprintingOsteogenic differentiationBone repair |
| spellingShingle | Rong Xu Hua Zhang Yang Luo Shiyi Pan Chi Zhang Xiaochuan Wu Guofeng Zhang Cuicui Su Dongdong Xia Magnetically bioprinted anisotropic hydrogels promote BMSC osteogenic differentiation for bone defect repair Materials Today Bio Anisotropic hydrogel Bioprinting Osteogenic differentiation Bone repair |
| title | Magnetically bioprinted anisotropic hydrogels promote BMSC osteogenic differentiation for bone defect repair |
| title_full | Magnetically bioprinted anisotropic hydrogels promote BMSC osteogenic differentiation for bone defect repair |
| title_fullStr | Magnetically bioprinted anisotropic hydrogels promote BMSC osteogenic differentiation for bone defect repair |
| title_full_unstemmed | Magnetically bioprinted anisotropic hydrogels promote BMSC osteogenic differentiation for bone defect repair |
| title_short | Magnetically bioprinted anisotropic hydrogels promote BMSC osteogenic differentiation for bone defect repair |
| title_sort | magnetically bioprinted anisotropic hydrogels promote bmsc osteogenic differentiation for bone defect repair |
| topic | Anisotropic hydrogel Bioprinting Osteogenic differentiation Bone repair |
| url | http://www.sciencedirect.com/science/article/pii/S2590006425004454 |
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