Molecular detection of some virulence gene in Proteus mirabilis isolated from chicken and human
The goal of this significant study is to isolate Proteus spp from both human and broiler sources and to compare the presence of the most significant genes responsible for virulence factors like zapA, rsp A, and ure C. This research, conducted from September 2023 to February 2024, collected twenty-fi...
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| Format: | Article |
| Language: | Arabic |
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University of Mosul, College of Veterinary Medicine
2025-01-01
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| Series: | Iraqi Journal of Veterinary Sciences |
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| Online Access: | https://www.vetmedmosul.com/article_185979_7ca7f5b87a2dfdd7a4897bda4e3f2aaa.pdf |
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| author | Aamer Y. Alchalaby Balsam Y. Rasheed Ammar M. Al-AAlim |
| author_facet | Aamer Y. Alchalaby Balsam Y. Rasheed Ammar M. Al-AAlim |
| author_sort | Aamer Y. Alchalaby |
| collection | DOAJ |
| description | The goal of this significant study is to isolate Proteus spp from both human and broiler sources and to compare the presence of the most significant genes responsible for virulence factors like zapA, rsp A, and ure C. This research, conducted from September 2023 to February 2024, collected twenty-five samples from the internal organs of broilers (heart, lung, liver, air sac, meat) and twenty-five samples from urinary tract infections in humans. The standard bacteriological method was used for bacterial identification, and PCR confirmed the Proteus spp isolates. Further sequencing and phylogenetic analysis of zap A were conducted. The study revealed 23 isolates of P. mirabilis from a total of 25 broiler samples, at a rate of 92%, while the isolation rate in humans was 88%, with 22 isolates from 25 urine samples. Molecular examination showed that all zap A, rsbA and ure C genes were found in both human and poultry isolates. The phylogenetic analysis shows that all Proteus isolates aggregate in one clade. In conclusion, our findings underscore the potential danger of virulence genes in poultry, a human staple food. This highlights the crucial need for caution in meat processing and the use of effective methods to prevent infection and sterilize meat before human consumption. |
| format | Article |
| id | doaj-art-cb1346af366949d3aba75945787e683c |
| institution | Kabale University |
| issn | 1607-3894 2071-1255 |
| language | Arabic |
| publishDate | 2025-01-01 |
| publisher | University of Mosul, College of Veterinary Medicine |
| record_format | Article |
| series | Iraqi Journal of Veterinary Sciences |
| spelling | doaj-art-cb1346af366949d3aba75945787e683c2025-02-04T13:11:42ZaraUniversity of Mosul, College of Veterinary MedicineIraqi Journal of Veterinary Sciences1607-38942071-12552025-01-01391535810.33899/ijvs.2024.153078.3857185979Molecular detection of some virulence gene in Proteus mirabilis isolated from chicken and humanAamer Y. Alchalaby0Balsam Y. Rasheed1Ammar M. Al-AAlim2Department of Microbiology, College of Veterinary Medicine, University of Mosul, Mosul, IraqDepartment of Microbiology, College of Veterinary Medicine, University of Mosul, Mosul, IraqDepartment of Microbiology, College of Veterinary Medicine, University of Mosul, Mosul, IraqThe goal of this significant study is to isolate Proteus spp from both human and broiler sources and to compare the presence of the most significant genes responsible for virulence factors like zapA, rsp A, and ure C. This research, conducted from September 2023 to February 2024, collected twenty-five samples from the internal organs of broilers (heart, lung, liver, air sac, meat) and twenty-five samples from urinary tract infections in humans. The standard bacteriological method was used for bacterial identification, and PCR confirmed the Proteus spp isolates. Further sequencing and phylogenetic analysis of zap A were conducted. The study revealed 23 isolates of P. mirabilis from a total of 25 broiler samples, at a rate of 92%, while the isolation rate in humans was 88%, with 22 isolates from 25 urine samples. Molecular examination showed that all zap A, rsbA and ure C genes were found in both human and poultry isolates. The phylogenetic analysis shows that all Proteus isolates aggregate in one clade. In conclusion, our findings underscore the potential danger of virulence genes in poultry, a human staple food. This highlights the crucial need for caution in meat processing and the use of effective methods to prevent infection and sterilize meat before human consumption.https://www.vetmedmosul.com/article_185979_7ca7f5b87a2dfdd7a4897bda4e3f2aaa.pdfmultiplex pcrproteus sppcrdbroiler |
| spellingShingle | Aamer Y. Alchalaby Balsam Y. Rasheed Ammar M. Al-AAlim Molecular detection of some virulence gene in Proteus mirabilis isolated from chicken and human Iraqi Journal of Veterinary Sciences multiplex pcr proteus spp crd broiler |
| title | Molecular detection of some virulence gene in Proteus mirabilis isolated from chicken and human |
| title_full | Molecular detection of some virulence gene in Proteus mirabilis isolated from chicken and human |
| title_fullStr | Molecular detection of some virulence gene in Proteus mirabilis isolated from chicken and human |
| title_full_unstemmed | Molecular detection of some virulence gene in Proteus mirabilis isolated from chicken and human |
| title_short | Molecular detection of some virulence gene in Proteus mirabilis isolated from chicken and human |
| title_sort | molecular detection of some virulence gene in proteus mirabilis isolated from chicken and human |
| topic | multiplex pcr proteus spp crd broiler |
| url | https://www.vetmedmosul.com/article_185979_7ca7f5b87a2dfdd7a4897bda4e3f2aaa.pdf |
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