Magnetically Controlled Transport of Nanoparticles in Solid Tumor Tissues and Porous Media Using a Tumor-on-a-Chip Format

This study addresses issues in developing spatially controlled magnetic fields for particle guidance, synthesizing biocompatible and chemically stable MNPs and enhancing their specificity to pathological cells through chemical modifications, developing personalized adjustments, and highlighting the...

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Main Authors: Tatiana Zimina, Nikita Sitkov, Ksenia Brusina, Viacheslav Fedorov, Natalia Mikhailova, Dmitriy Testov, Kamil Gareev, Konstantin Samochernykh, Stephanie Combs, Maxim Shevtsov
Format: Article
Language:English
Published: MDPI AG 2024-12-01
Series:Nanomaterials
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Online Access:https://www.mdpi.com/2079-4991/14/24/2030
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author Tatiana Zimina
Nikita Sitkov
Ksenia Brusina
Viacheslav Fedorov
Natalia Mikhailova
Dmitriy Testov
Kamil Gareev
Konstantin Samochernykh
Stephanie Combs
Maxim Shevtsov
author_facet Tatiana Zimina
Nikita Sitkov
Ksenia Brusina
Viacheslav Fedorov
Natalia Mikhailova
Dmitriy Testov
Kamil Gareev
Konstantin Samochernykh
Stephanie Combs
Maxim Shevtsov
author_sort Tatiana Zimina
collection DOAJ
description This study addresses issues in developing spatially controlled magnetic fields for particle guidance, synthesizing biocompatible and chemically stable MNPs and enhancing their specificity to pathological cells through chemical modifications, developing personalized adjustments, and highlighting the potential of tumor-on-a-chip systems, which can simulate tissue environments and assess drug efficacy and dosage in a controlled setting. The research focused on two MNP types, uncoated magnetite nanoparticles (mMNPs) and carboxymethyl dextran coated superparamagnetic nanoparticles (CD-SPIONs), and evaluated their transport properties in microfluidic systems and porous media. The original uncoated mMNPs of bimodal size distribution and the narrow size distribution of the fractions (23 nm and 106 nm by radii) were demonstrated to agglomerate in magnetically driven microfluidic flow, forming a stable stationary web consisting of magnetic fibers within 30 min. CD-SPIONs were demonstrated to migrate in agar gel with the mean pore size equal to or slightly higher than the particle size. The migration velocity was inversely proportional to the size of particles. No compression of the gel was observed under the magnetic field gradient of 40 T/m. In the brain tissue, particles of sizes 220, 350, 820 nm were not penetrating the tissue, while the compression of tissue was observed. The particles of 95 nm size penetrated the tissue at the edge of the sample, and no compression was observed. For all particles, movement through capillary vessels was observed.
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spelling doaj-art-cb111761219544d8a53dfd6c7fe3c7952025-08-20T02:43:20ZengMDPI AGNanomaterials2079-49912024-12-011424203010.3390/nano14242030Magnetically Controlled Transport of Nanoparticles in Solid Tumor Tissues and Porous Media Using a Tumor-on-a-Chip FormatTatiana Zimina0Nikita Sitkov1Ksenia Brusina2Viacheslav Fedorov3Natalia Mikhailova4Dmitriy Testov5Kamil Gareev6Konstantin Samochernykh7Stephanie Combs8Maxim Shevtsov9Department of Micro and Nanoelectronics, St. Petersburg Electrotechnical University “LETI” (ETU “LETI”), Prof. Popova Str., 5, 197022 St. Petersburg, RussiaPersonalized Medicine Centre, Almazov National Medical Research Centre, Akkuratova Str. 2, 197341 St. Petersburg, RussiaDepartment of Micro and Nanoelectronics, St. Petersburg Electrotechnical University “LETI” (ETU “LETI”), Prof. Popova Str., 5, 197022 St. Petersburg, RussiaPersonalized Medicine Centre, Almazov National Medical Research Centre, Akkuratova Str. 2, 197341 St. Petersburg, RussiaPersonalized Medicine Centre, Almazov National Medical Research Centre, Akkuratova Str. 2, 197341 St. Petersburg, RussiaDepartment of Micro and Nanoelectronics, St. Petersburg Electrotechnical University “LETI” (ETU “LETI”), Prof. Popova Str., 5, 197022 St. Petersburg, RussiaPersonalized Medicine Centre, Almazov National Medical Research Centre, Akkuratova Str. 2, 197341 St. Petersburg, RussiaPersonalized Medicine Centre, Almazov National Medical Research Centre, Akkuratova Str. 2, 197341 St. Petersburg, RussiaDepartment of Radiation Oncology, Technische Universität München (TUM), Klinikum Rechts der Isar, Ismaninger Str. 22, 81675 Munich, GermanyPersonalized Medicine Centre, Almazov National Medical Research Centre, Akkuratova Str. 2, 197341 St. Petersburg, RussiaThis study addresses issues in developing spatially controlled magnetic fields for particle guidance, synthesizing biocompatible and chemically stable MNPs and enhancing their specificity to pathological cells through chemical modifications, developing personalized adjustments, and highlighting the potential of tumor-on-a-chip systems, which can simulate tissue environments and assess drug efficacy and dosage in a controlled setting. The research focused on two MNP types, uncoated magnetite nanoparticles (mMNPs) and carboxymethyl dextran coated superparamagnetic nanoparticles (CD-SPIONs), and evaluated their transport properties in microfluidic systems and porous media. The original uncoated mMNPs of bimodal size distribution and the narrow size distribution of the fractions (23 nm and 106 nm by radii) were demonstrated to agglomerate in magnetically driven microfluidic flow, forming a stable stationary web consisting of magnetic fibers within 30 min. CD-SPIONs were demonstrated to migrate in agar gel with the mean pore size equal to or slightly higher than the particle size. The migration velocity was inversely proportional to the size of particles. No compression of the gel was observed under the magnetic field gradient of 40 T/m. In the brain tissue, particles of sizes 220, 350, 820 nm were not penetrating the tissue, while the compression of tissue was observed. The particles of 95 nm size penetrated the tissue at the edge of the sample, and no compression was observed. For all particles, movement through capillary vessels was observed.https://www.mdpi.com/2079-4991/14/24/2030tumormagnetic nanoparticlesmagnetically controlled transportmicrofluidic systemsorganic porous systemstissue engineering
spellingShingle Tatiana Zimina
Nikita Sitkov
Ksenia Brusina
Viacheslav Fedorov
Natalia Mikhailova
Dmitriy Testov
Kamil Gareev
Konstantin Samochernykh
Stephanie Combs
Maxim Shevtsov
Magnetically Controlled Transport of Nanoparticles in Solid Tumor Tissues and Porous Media Using a Tumor-on-a-Chip Format
Nanomaterials
tumor
magnetic nanoparticles
magnetically controlled transport
microfluidic systems
organic porous systems
tissue engineering
title Magnetically Controlled Transport of Nanoparticles in Solid Tumor Tissues and Porous Media Using a Tumor-on-a-Chip Format
title_full Magnetically Controlled Transport of Nanoparticles in Solid Tumor Tissues and Porous Media Using a Tumor-on-a-Chip Format
title_fullStr Magnetically Controlled Transport of Nanoparticles in Solid Tumor Tissues and Porous Media Using a Tumor-on-a-Chip Format
title_full_unstemmed Magnetically Controlled Transport of Nanoparticles in Solid Tumor Tissues and Porous Media Using a Tumor-on-a-Chip Format
title_short Magnetically Controlled Transport of Nanoparticles in Solid Tumor Tissues and Porous Media Using a Tumor-on-a-Chip Format
title_sort magnetically controlled transport of nanoparticles in solid tumor tissues and porous media using a tumor on a chip format
topic tumor
magnetic nanoparticles
magnetically controlled transport
microfluidic systems
organic porous systems
tissue engineering
url https://www.mdpi.com/2079-4991/14/24/2030
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