Comparison of the Minimum Inhibitory and Mutant Prevention Drug Concentrations for Pradofloxacin and 7 Other Antimicrobial Agents Tested Against Swine Isolates of <i>Actinobacillus pleuropneumoniae</i> and <i>Pasteurella multocida</i>
Pradofloxacin is a dual targeting, bactericidal fluoroquinolone recently approved for treating bacteria causing swine respiratory disease. Currently, an abundance of in vitro data does not exist for pradofloxacin. We determined the minimum inhibitory concentration (MIC) and mutant prevention concent...
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2024-11-01
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| author | Joseph M. Blondeau Shantelle D. Fitch |
| author_facet | Joseph M. Blondeau Shantelle D. Fitch |
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| description | Pradofloxacin is a dual targeting, bactericidal fluoroquinolone recently approved for treating bacteria causing swine respiratory disease. Currently, an abundance of in vitro data does not exist for pradofloxacin. We determined the minimum inhibitory concentration (MIC) and mutant prevention concentrations (MPC) of pradofloxacin compared to ceftiofur, enrofloxacin, florfenicol, marbofloxacin, tildipirosin, tilmicosin and tulathromycin against swine isolates of <i>Actinobacillus pleuropneumoniae</i> and <i>Pasteurella multocida</i>. Overall, pradofloxacin had the lowest MIC and MPC values as compared to the other agents tested. For example, pradofloxacin MIC values for 50%, 90% and 100% of <i>A. pleuropneumoniae</i> strains were ≤0.016 µg/mL, ≤0.016 µg/mL and ≤0.016 µg/mL and for <i>P. multocida</i> were ≤0.016 µg/mL, ≤0.016 µg/mL and 0.031 µg/mL, respectively. The MPC values for 50%, 90% and 100% of <i>A. pleuropneumoniae</i> strains were 0.031 µg/mL, 0.063 µg/mL and 0.125 µg/mL and for <i>P. multocida</i> were ≤0.016 µg/mL, 0.031 µg/mL and 0.0.063 µg/mL, respectively. By MPC testing, all strains were at or below the susceptibility breakpoint. Based on MPC testing, pradofloxacin appears to have a low likelihood for resistance selection. This study represents the most comprehensive in vitro comparison of the above noted drugs and the first report for pradofloxacin and tildipirosin. |
| format | Article |
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| spelling | doaj-art-cb0c0a2eeb444745973c9511afa9f6fc2025-08-20T01:53:54ZengMDPI AGMolecules1420-30492024-11-012922544810.3390/molecules29225448Comparison of the Minimum Inhibitory and Mutant Prevention Drug Concentrations for Pradofloxacin and 7 Other Antimicrobial Agents Tested Against Swine Isolates of <i>Actinobacillus pleuropneumoniae</i> and <i>Pasteurella multocida</i>Joseph M. Blondeau0Shantelle D. Fitch1Departments of Biochemistry, Microbiology and Immunology, Pathology and Laboratory Medicine and Ophthalmology, University of Saskatchewan, Saskatoon, SK S7N 0W8, CanadaDepartment of Clinical Microbiology, Royal University Hospital and Saskatchewan Health Authority, Saskatoon, SK S7N 0W8, CanadaPradofloxacin is a dual targeting, bactericidal fluoroquinolone recently approved for treating bacteria causing swine respiratory disease. Currently, an abundance of in vitro data does not exist for pradofloxacin. We determined the minimum inhibitory concentration (MIC) and mutant prevention concentrations (MPC) of pradofloxacin compared to ceftiofur, enrofloxacin, florfenicol, marbofloxacin, tildipirosin, tilmicosin and tulathromycin against swine isolates of <i>Actinobacillus pleuropneumoniae</i> and <i>Pasteurella multocida</i>. Overall, pradofloxacin had the lowest MIC and MPC values as compared to the other agents tested. For example, pradofloxacin MIC values for 50%, 90% and 100% of <i>A. pleuropneumoniae</i> strains were ≤0.016 µg/mL, ≤0.016 µg/mL and ≤0.016 µg/mL and for <i>P. multocida</i> were ≤0.016 µg/mL, ≤0.016 µg/mL and 0.031 µg/mL, respectively. The MPC values for 50%, 90% and 100% of <i>A. pleuropneumoniae</i> strains were 0.031 µg/mL, 0.063 µg/mL and 0.125 µg/mL and for <i>P. multocida</i> were ≤0.016 µg/mL, 0.031 µg/mL and 0.0.063 µg/mL, respectively. By MPC testing, all strains were at or below the susceptibility breakpoint. Based on MPC testing, pradofloxacin appears to have a low likelihood for resistance selection. This study represents the most comprehensive in vitro comparison of the above noted drugs and the first report for pradofloxacin and tildipirosin.https://www.mdpi.com/1420-3049/29/22/5448<i>Actinobacillus pleuropneumoniae</i><i>Pasteurella multocida</i>MIC/MPCpradofloxacin |
| spellingShingle | Joseph M. Blondeau Shantelle D. Fitch Comparison of the Minimum Inhibitory and Mutant Prevention Drug Concentrations for Pradofloxacin and 7 Other Antimicrobial Agents Tested Against Swine Isolates of <i>Actinobacillus pleuropneumoniae</i> and <i>Pasteurella multocida</i> Molecules <i>Actinobacillus pleuropneumoniae</i> <i>Pasteurella multocida</i> MIC/MPC pradofloxacin |
| title | Comparison of the Minimum Inhibitory and Mutant Prevention Drug Concentrations for Pradofloxacin and 7 Other Antimicrobial Agents Tested Against Swine Isolates of <i>Actinobacillus pleuropneumoniae</i> and <i>Pasteurella multocida</i> |
| title_full | Comparison of the Minimum Inhibitory and Mutant Prevention Drug Concentrations for Pradofloxacin and 7 Other Antimicrobial Agents Tested Against Swine Isolates of <i>Actinobacillus pleuropneumoniae</i> and <i>Pasteurella multocida</i> |
| title_fullStr | Comparison of the Minimum Inhibitory and Mutant Prevention Drug Concentrations for Pradofloxacin and 7 Other Antimicrobial Agents Tested Against Swine Isolates of <i>Actinobacillus pleuropneumoniae</i> and <i>Pasteurella multocida</i> |
| title_full_unstemmed | Comparison of the Minimum Inhibitory and Mutant Prevention Drug Concentrations for Pradofloxacin and 7 Other Antimicrobial Agents Tested Against Swine Isolates of <i>Actinobacillus pleuropneumoniae</i> and <i>Pasteurella multocida</i> |
| title_short | Comparison of the Minimum Inhibitory and Mutant Prevention Drug Concentrations for Pradofloxacin and 7 Other Antimicrobial Agents Tested Against Swine Isolates of <i>Actinobacillus pleuropneumoniae</i> and <i>Pasteurella multocida</i> |
| title_sort | comparison of the minimum inhibitory and mutant prevention drug concentrations for pradofloxacin and 7 other antimicrobial agents tested against swine isolates of i actinobacillus pleuropneumoniae i and i pasteurella multocida i |
| topic | <i>Actinobacillus pleuropneumoniae</i> <i>Pasteurella multocida</i> MIC/MPC pradofloxacin |
| url | https://www.mdpi.com/1420-3049/29/22/5448 |
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