The influence of Quercetin on behavior, performance and splenic immunity in broiler chickens

Quercetin (QRT), a potent flavonoid, holds immense mechanistic potential in enhancing various aspects of broiler chicken physiological status. This comprehensive study explores the profound еffеcts of QRT on behavior, productive performance, and immune functions, unraveling the underlying mechanisms...

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Main Authors: Sara Elsayed El-Kazaz, Mona Hafez Hafez, Ghadeer M. Albadrani, Muath Q. Al-Ghadi, Mohamed M. Abdel-Daim, Yasser Said El-Sayed
Format: Article
Language:English
Published: Elsevier 2024-12-01
Series:Veterinary and Animal Science
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Online Access:http://www.sciencedirect.com/science/article/pii/S2451943X24000656
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author Sara Elsayed El-Kazaz
Mona Hafez Hafez
Ghadeer M. Albadrani
Muath Q. Al-Ghadi
Mohamed M. Abdel-Daim
Yasser Said El-Sayed
author_facet Sara Elsayed El-Kazaz
Mona Hafez Hafez
Ghadeer M. Albadrani
Muath Q. Al-Ghadi
Mohamed M. Abdel-Daim
Yasser Said El-Sayed
author_sort Sara Elsayed El-Kazaz
collection DOAJ
description Quercetin (QRT), a potent flavonoid, holds immense mechanistic potential in enhancing various aspects of broiler chicken physiological status. This comprehensive study explores the profound еffеcts of QRT on behavior, productive performance, and immune functions, unraveling the underlying mechanisms behind its efficacy. Four hundred, one-day-old Cobb 500 chicks were placed randomly into 4 supplementation groups (100 birds /group and five replicates) and provided diets enriched with varying concentrations of QRT (0, 200, 400, and 600 ppm) for six weeks. Visual scanning revealed significant (P > 0.05) improvements in feeding and body care behaviors, accompanied by reduced instances of idleness and walking in the QRT-supplemented groups. Moreover, QRT supplementation exerted a substantial (P > 0.05) positive influence on weight gain, feed intake and the final body weight of the broilers. In-depth evaluation of immune parameters, QRT supplementation elicited significant (P > 0.05) enhancements in immune functions, including improved spleen, thymus, and bursa indices, enhanced secretion of immunoglobulin M (IgM) and immunoglobulin A (IgA), and stimulated humoral immunity against sheep red blood cells (SRBCs). Furthermore, QRT displays potent antioxidant properties, as showed by diminished splenic malondialdehyde levels and augmented activity of antioxidative stress enzymes. Remarkably, QRT supplementation elicited dose-dependent upregulation (P < 0.001) of key immune-related genes, such as intеrlеukin-4 (IL-4), interferon-γ (INF-γ), Toll-like receptor 2 (TLR2), and tumor necrosis factor-α (TNF-α) in the splenic tissue. Collectively, these mechanistic insights underscore the profound impact of QRT as a functional feed additive, fostering enhanced behavior, performance, and immune function in broiler chickens, while delivering robust antioxidant fortification.
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spelling doaj-art-caf69e8f42d745bea4e24bf7659e5bb62025-08-20T02:48:39ZengElsevierVeterinary and Animal Science2451-943X2024-12-012610039810.1016/j.vas.2024.100398The influence of Quercetin on behavior, performance and splenic immunity in broiler chickensSara Elsayed El-Kazaz0Mona Hafez Hafez1Ghadeer M. Albadrani2Muath Q. Al-Ghadi3Mohamed M. Abdel-Daim4Yasser Said El-Sayed5Animal Husbandry and Animal Wealth Development Department, Faculty of Veterinary Medicine, Alexandria University, Alexandria, 22758, EgyptPhysiology Department, Faculty of Veterinary Medicine, Alexandria University, Alexandria, 22758, Egypt; Corresponding author at: Physiology Department, Faculty of Veterinary Medicine, Alexandria University, Alexandria, 22758, Egypt.Department of Biology, College of Science, Princess Nourah bint Abdulrahman University, 84428, Riyadh 11671, Saudi ArabiaDepartment of Zoology, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi ArabiaDepartment of Pharmaceutical Sciences, Pharmacy Program, Batterjee Medical College, P.O. Box 6231 Jeddah 21442, Saudi Arabia; Pharmacology Department, Faculty of Veterinary Medicine, Suez Canal University, Ismailia 41522, EgyptForensic Medicine and Toxicology Department, Faculty of Veterinary Medicine, Damanhour University, Damanhour, EgyptQuercetin (QRT), a potent flavonoid, holds immense mechanistic potential in enhancing various aspects of broiler chicken physiological status. This comprehensive study explores the profound еffеcts of QRT on behavior, productive performance, and immune functions, unraveling the underlying mechanisms behind its efficacy. Four hundred, one-day-old Cobb 500 chicks were placed randomly into 4 supplementation groups (100 birds /group and five replicates) and provided diets enriched with varying concentrations of QRT (0, 200, 400, and 600 ppm) for six weeks. Visual scanning revealed significant (P > 0.05) improvements in feeding and body care behaviors, accompanied by reduced instances of idleness and walking in the QRT-supplemented groups. Moreover, QRT supplementation exerted a substantial (P > 0.05) positive influence on weight gain, feed intake and the final body weight of the broilers. In-depth evaluation of immune parameters, QRT supplementation elicited significant (P > 0.05) enhancements in immune functions, including improved spleen, thymus, and bursa indices, enhanced secretion of immunoglobulin M (IgM) and immunoglobulin A (IgA), and stimulated humoral immunity against sheep red blood cells (SRBCs). Furthermore, QRT displays potent antioxidant properties, as showed by diminished splenic malondialdehyde levels and augmented activity of antioxidative stress enzymes. Remarkably, QRT supplementation elicited dose-dependent upregulation (P < 0.001) of key immune-related genes, such as intеrlеukin-4 (IL-4), interferon-γ (INF-γ), Toll-like receptor 2 (TLR2), and tumor necrosis factor-α (TNF-α) in the splenic tissue. Collectively, these mechanistic insights underscore the profound impact of QRT as a functional feed additive, fostering enhanced behavior, performance, and immune function in broiler chickens, while delivering robust antioxidant fortification.http://www.sciencedirect.com/science/article/pii/S2451943X24000656Behavior and productive performanceBroiler chickenGene expressionImmune functionOxidative stressQuercetin
spellingShingle Sara Elsayed El-Kazaz
Mona Hafez Hafez
Ghadeer M. Albadrani
Muath Q. Al-Ghadi
Mohamed M. Abdel-Daim
Yasser Said El-Sayed
The influence of Quercetin on behavior, performance and splenic immunity in broiler chickens
Veterinary and Animal Science
Behavior and productive performance
Broiler chicken
Gene expression
Immune function
Oxidative stress
Quercetin
title The influence of Quercetin on behavior, performance and splenic immunity in broiler chickens
title_full The influence of Quercetin on behavior, performance and splenic immunity in broiler chickens
title_fullStr The influence of Quercetin on behavior, performance and splenic immunity in broiler chickens
title_full_unstemmed The influence of Quercetin on behavior, performance and splenic immunity in broiler chickens
title_short The influence of Quercetin on behavior, performance and splenic immunity in broiler chickens
title_sort influence of quercetin on behavior performance and splenic immunity in broiler chickens
topic Behavior and productive performance
Broiler chicken
Gene expression
Immune function
Oxidative stress
Quercetin
url http://www.sciencedirect.com/science/article/pii/S2451943X24000656
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