Frequency of Antibiotic-Resistant Bacteria isolated from the Kınalıada Coastal Areas of the Sea of Marmara, Türkiye
In the process of global climate change, the negative effects of anthropogenic activities on microbial interactions have become more visible in coastal areas. Because island coastal ecosystems are fragile ecosystems that are open to dynamic environmental variables, it is important to determine bacte...
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Istanbul University Press
2024-10-01
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| Series: | Aquatic Sciences and Engineering |
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| Online Access: | https://dergipark.org.tr/en/download/article-file/3773506 |
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| author | Pelin Saliha Çiftçi Türetken Gülşen Altuğ Selma Dilara Karaman Baş |
| author_facet | Pelin Saliha Çiftçi Türetken Gülşen Altuğ Selma Dilara Karaman Baş |
| author_sort | Pelin Saliha Çiftçi Türetken |
| collection | DOAJ |
| description | In the process of global climate change, the negative effects of anthropogenic activities on microbial interactions have become more visible in coastal areas. Because island coastal ecosystems are fragile ecosystems that are open to dynamic environmental variables, it is important to determine bacteriological signals in these regions. The frequency of bacterial antibiotic resistance in aquatic ecosystems is a micro-marker of human activity. The frequency of antibiotic-resistant bacteria was investigated in surface water samples collected from the coastal areas of Kınalıada Island in the Sea of Marmara between 2018 and 2019. The bacteria isolated from the sea water were screened against: spectinomycin (SC300), nitrofurantoin (F50), Rifampicin (Rd2), tetracycline (TE30), ampicillin (AMP10), and oxytetracycline (OT30) using the disk diffusion technique. The frequencies of antibiotic-resistant faecal coliform, total coliform, intestinal enterococcus, and heterotrophic aerobic bacteria were evaluated according to the Clinical Laboratory Standard Institute (CLSI). The antibiotics to which all bacterial isolates showed the highest resistance were tetracycline and oxytetracycline (98.7% The frequency of resistant heterotrophic aerobic bacteria was recorded at 100% against all tested antibiotics. All bacterial isolates showed resistance to more than three antibiotic derivatives, and the Multiple Antibiotic Resistance (MAR) index was determined to be in the range of 0.67–1. The findings of this study provide regional evidence of the influence of anthropogenic pollution on the spread of antibiotic resistance. The detection of high levels of antibiotic-resistant bacteria indicated that the coastal areas of Kınalıada are at potential risk for the global spread of resistant bacteria, human health, and ecosystem function. |
| format | Article |
| id | doaj-art-caec375f860b4ffd87b42706b98dee2d |
| institution | Kabale University |
| issn | 2602-473X |
| language | English |
| publishDate | 2024-10-01 |
| publisher | Istanbul University Press |
| record_format | Article |
| series | Aquatic Sciences and Engineering |
| spelling | doaj-art-caec375f860b4ffd87b42706b98dee2d2025-02-04T08:44:52ZengIstanbul University PressAquatic Sciences and Engineering2602-473X2024-10-0139421622110.26650/ASE202414473414Frequency of Antibiotic-Resistant Bacteria isolated from the Kınalıada Coastal Areas of the Sea of Marmara, TürkiyePelin Saliha Çiftçi Türetken0https://orcid.org/0000-0002-4377-1628Gülşen Altuğ1https://orcid.org/0000-0003-3251-7699Selma Dilara Karaman Baş2https://orcid.org/0000-0002-8682-4742ISTANBUL UNIVERSITY, FACULTY OF AQUATIC SCIENCES, DEPARTMENT OF MARINE BIOLOGYISTANBUL UNIVERSITY, FACULTY OF AQUATIC SCIENCES, DEPARTMENT OF MARINE BIOLOGYIstanbul University, Institute of Graduate Studies in SciencesIn the process of global climate change, the negative effects of anthropogenic activities on microbial interactions have become more visible in coastal areas. Because island coastal ecosystems are fragile ecosystems that are open to dynamic environmental variables, it is important to determine bacteriological signals in these regions. The frequency of bacterial antibiotic resistance in aquatic ecosystems is a micro-marker of human activity. The frequency of antibiotic-resistant bacteria was investigated in surface water samples collected from the coastal areas of Kınalıada Island in the Sea of Marmara between 2018 and 2019. The bacteria isolated from the sea water were screened against: spectinomycin (SC300), nitrofurantoin (F50), Rifampicin (Rd2), tetracycline (TE30), ampicillin (AMP10), and oxytetracycline (OT30) using the disk diffusion technique. The frequencies of antibiotic-resistant faecal coliform, total coliform, intestinal enterococcus, and heterotrophic aerobic bacteria were evaluated according to the Clinical Laboratory Standard Institute (CLSI). The antibiotics to which all bacterial isolates showed the highest resistance were tetracycline and oxytetracycline (98.7% The frequency of resistant heterotrophic aerobic bacteria was recorded at 100% against all tested antibiotics. All bacterial isolates showed resistance to more than three antibiotic derivatives, and the Multiple Antibiotic Resistance (MAR) index was determined to be in the range of 0.67–1. The findings of this study provide regional evidence of the influence of anthropogenic pollution on the spread of antibiotic resistance. The detection of high levels of antibiotic-resistant bacteria indicated that the coastal areas of Kınalıada are at potential risk for the global spread of resistant bacteria, human health, and ecosystem function.https://dergipark.org.tr/en/download/article-file/3773506the sea of marmarakınalıada islandbio-indicator bacteriaantibiotic resistant bacteria |
| spellingShingle | Pelin Saliha Çiftçi Türetken Gülşen Altuğ Selma Dilara Karaman Baş Frequency of Antibiotic-Resistant Bacteria isolated from the Kınalıada Coastal Areas of the Sea of Marmara, Türkiye Aquatic Sciences and Engineering the sea of marmara kınalıada island bio-indicator bacteria antibiotic resistant bacteria |
| title | Frequency of Antibiotic-Resistant Bacteria isolated from the Kınalıada Coastal Areas of the Sea of Marmara, Türkiye |
| title_full | Frequency of Antibiotic-Resistant Bacteria isolated from the Kınalıada Coastal Areas of the Sea of Marmara, Türkiye |
| title_fullStr | Frequency of Antibiotic-Resistant Bacteria isolated from the Kınalıada Coastal Areas of the Sea of Marmara, Türkiye |
| title_full_unstemmed | Frequency of Antibiotic-Resistant Bacteria isolated from the Kınalıada Coastal Areas of the Sea of Marmara, Türkiye |
| title_short | Frequency of Antibiotic-Resistant Bacteria isolated from the Kınalıada Coastal Areas of the Sea of Marmara, Türkiye |
| title_sort | frequency of antibiotic resistant bacteria isolated from the kinaliada coastal areas of the sea of marmara turkiye |
| topic | the sea of marmara kınalıada island bio-indicator bacteria antibiotic resistant bacteria |
| url | https://dergipark.org.tr/en/download/article-file/3773506 |
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