Mapping of Minimal Motifs of B-Cell Epitopes on Human Zona Pellucida Glycoprotein-3
The human zona pellucida glycoprotein-3 (hZP3) by virtue of its critical role during fertilization has been proposed as a promising candidate antigen to develop a contraceptive vaccine. In this direction, it is imperative to map minimal motifs of the B cell epitopes (BCEs) so as to avoid ZP-specific...
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| Format: | Article |
| Language: | English |
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Wiley
2012-01-01
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| Series: | Clinical and Developmental Immunology |
| Online Access: | http://dx.doi.org/10.1155/2012/831010 |
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| author | Wan-Xiang Xu Ya-Ping He Jian Wang Hai-Ping Tang Hui-Juan Shi Xiao-Xi Sun Chao-Neng Ji Shao-Hua Gu Yi Xie |
| author_facet | Wan-Xiang Xu Ya-Ping He Jian Wang Hai-Ping Tang Hui-Juan Shi Xiao-Xi Sun Chao-Neng Ji Shao-Hua Gu Yi Xie |
| author_sort | Wan-Xiang Xu |
| collection | DOAJ |
| description | The human zona pellucida glycoprotein-3 (hZP3) by virtue of its critical role during fertilization has been proposed as a promising candidate antigen to develop a contraceptive vaccine. In this direction, it is imperative to map minimal motifs of the B cell epitopes (BCEs) so as to avoid ZP-specific oophoritogenic T cell epitopes (TCEs) in the ZP3-based immunogens. In this study, based on known results of mapping marmoset and bonnet monkey ZP3 (mstZP3 and bmZP3), two predictable epitopes23–30 and 301–320 on hZP3 were first confirmed and five minimal motifs within four epitopes on hZP3 were defined using serum to recombinant hZP3a22–176 or hZP3b177–348 as well as a biosynthetic peptide strategy. These defined minimal motifs were QPLWLL23–28 for hZP323–30, MQVTDD103–108 for hZP393–110, EENW178–181 for hZP3172–190, as well as SNSWF306–310 and EGP313–315 for hZP3301–320, respectively. Furthermore, the antigenicity of two peptides for hZP3172–187 and hZP3301–315 and specificity of the antibody response to these peptides were also evaluated, which produced high-titer antibodies in immunized animals that were capable of reacting to ZP on human oocytes, r-hZP3b177–348 protein, as well as r-hZP3172–190, r-hZP3303–310, and r-hZP3313–320 epitope peptides fused with truncated GST188 protein. |
| format | Article |
| id | doaj-art-cacfcfccb46f40d3978b487b6138b40d |
| institution | Kabale University |
| issn | 1740-2522 1740-2530 |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Clinical and Developmental Immunology |
| spelling | doaj-art-cacfcfccb46f40d3978b487b6138b40d2025-02-03T00:59:52ZengWileyClinical and Developmental Immunology1740-25221740-25302012-01-01201210.1155/2012/831010831010Mapping of Minimal Motifs of B-Cell Epitopes on Human Zona Pellucida Glycoprotein-3Wan-Xiang Xu0Ya-Ping He1Jian Wang2Hai-Ping Tang3Hui-Juan Shi4Xiao-Xi Sun5Chao-Neng Ji6Shao-Hua Gu7Yi Xie8Department of Reproductive Biology, Shanghai Institute of Planned Parenthood Research, Shanghai 200032, ChinaDepartment of Reproductive Biology, Shanghai Institute of Planned Parenthood Research, Shanghai 200032, ChinaDepartment of Reproductive Biology, Shanghai Institute of Planned Parenthood Research, Shanghai 200032, ChinaDepartment of Reproductive Biology, Shanghai Institute of Planned Parenthood Research, Shanghai 200032, ChinaDepartment of Reproductive Biology, Shanghai Institute of Planned Parenthood Research, Shanghai 200032, ChinaShanghai Jiai Genetic & IVF Center, Obstetrics and Gynecology Hospital, Shanghai Medical College, Fudan University, Shanghai 200011, ChinaState Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Science, Fudan University, Shanghai 200433, ChinaState Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Science, Fudan University, Shanghai 200433, ChinaState Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Science, Fudan University, Shanghai 200433, ChinaThe human zona pellucida glycoprotein-3 (hZP3) by virtue of its critical role during fertilization has been proposed as a promising candidate antigen to develop a contraceptive vaccine. In this direction, it is imperative to map minimal motifs of the B cell epitopes (BCEs) so as to avoid ZP-specific oophoritogenic T cell epitopes (TCEs) in the ZP3-based immunogens. In this study, based on known results of mapping marmoset and bonnet monkey ZP3 (mstZP3 and bmZP3), two predictable epitopes23–30 and 301–320 on hZP3 were first confirmed and five minimal motifs within four epitopes on hZP3 were defined using serum to recombinant hZP3a22–176 or hZP3b177–348 as well as a biosynthetic peptide strategy. These defined minimal motifs were QPLWLL23–28 for hZP323–30, MQVTDD103–108 for hZP393–110, EENW178–181 for hZP3172–190, as well as SNSWF306–310 and EGP313–315 for hZP3301–320, respectively. Furthermore, the antigenicity of two peptides for hZP3172–187 and hZP3301–315 and specificity of the antibody response to these peptides were also evaluated, which produced high-titer antibodies in immunized animals that were capable of reacting to ZP on human oocytes, r-hZP3b177–348 protein, as well as r-hZP3172–190, r-hZP3303–310, and r-hZP3313–320 epitope peptides fused with truncated GST188 protein.http://dx.doi.org/10.1155/2012/831010 |
| spellingShingle | Wan-Xiang Xu Ya-Ping He Jian Wang Hai-Ping Tang Hui-Juan Shi Xiao-Xi Sun Chao-Neng Ji Shao-Hua Gu Yi Xie Mapping of Minimal Motifs of B-Cell Epitopes on Human Zona Pellucida Glycoprotein-3 Clinical and Developmental Immunology |
| title | Mapping of Minimal Motifs of B-Cell Epitopes on Human Zona Pellucida Glycoprotein-3 |
| title_full | Mapping of Minimal Motifs of B-Cell Epitopes on Human Zona Pellucida Glycoprotein-3 |
| title_fullStr | Mapping of Minimal Motifs of B-Cell Epitopes on Human Zona Pellucida Glycoprotein-3 |
| title_full_unstemmed | Mapping of Minimal Motifs of B-Cell Epitopes on Human Zona Pellucida Glycoprotein-3 |
| title_short | Mapping of Minimal Motifs of B-Cell Epitopes on Human Zona Pellucida Glycoprotein-3 |
| title_sort | mapping of minimal motifs of b cell epitopes on human zona pellucida glycoprotein 3 |
| url | http://dx.doi.org/10.1155/2012/831010 |
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