Mass Cultivation of Entomopathogenic Nematode in Artificial Media
<p>Entomopathogenic nematodes Entomopathogenic nematodes (EPN) of the genera Heterorhabditis and Steinernema are commercially used to control pest insect. EPN is widely cultivated through in-vivo and in vitro methods. This research aims to discover the abundance of EPN cultivated in various ar...
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Universitas Negeri Semarang
2016-03-01
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| Series: | Biosaintifika: Journal of Biology & Biology Education |
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| Online Access: | https://journal.unnes.ac.id/nju/index.php/biosaintifika/article/view/5579 |
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| author | Dyah Rini Indriyanti Nur Lailatul Muharromah |
| author_facet | Dyah Rini Indriyanti Nur Lailatul Muharromah |
| author_sort | Dyah Rini Indriyanti |
| collection | DOAJ |
| description | <p>Entomopathogenic nematodes Entomopathogenic nematodes (EPN) of the genera Heterorhabditis and Steinernema are commercially used to control pest insect. EPN is widely cultivated through in-vivo and in vitro methods. This research aims to discover the abundance of EPN cultivated in various artificial media. Seven types of media composition were tested in this research: media A (yeast + soybean powder), media B (yeast + chicken liver), media C (yeast + dog food), media D (yolk + soybean powder), media E (yolk + chicken liver), media F (yolk + dog food), and media G (yeast + yolk + dog food). Each media was inoculated in 1.2x103JI/mL. The growth of EPN was observed weekly in 4 weeks. Results showed that EPN could be cultivated using various media; media D, E, F, and G. Highest abundance of EPN is found in the second week of media D for 28164 JI/ml.Cell harvesting is suggested to be conducted during the first and second week to obtain maximum abundance of EPN.</p><p><strong>How to Cite</strong></p><p>Indriyanti, D., & Muharromah, N. (2016). Mass Cultivation of Entomopathogenic Nematode In Artificial Media.<em> Biosaintifika: Journal of Biology & Biology Education</em>, 8(1), 111-118.</p> |
| format | Article |
| id | doaj-art-caccd6918fd0452e868d5600f38f35d3 |
| institution | DOAJ |
| issn | 2085-191X 2338-7610 |
| language | English |
| publishDate | 2016-03-01 |
| publisher | Universitas Negeri Semarang |
| record_format | Article |
| series | Biosaintifika: Journal of Biology & Biology Education |
| spelling | doaj-art-caccd6918fd0452e868d5600f38f35d32025-08-20T03:24:03ZengUniversitas Negeri SemarangBiosaintifika: Journal of Biology & Biology Education2085-191X2338-76102016-03-018111312010.15294/biosaintifika.v8i1.55794267Mass Cultivation of Entomopathogenic Nematode in Artificial MediaDyah Rini Indriyanti0Nur Lailatul Muharromah1Department of Biology, Faculty of Mathematics and Sciences, Semarang State University, IndonesiaDepartment of Biology, Faculty of Mathematics and Sciences, Semarang State University, Indonesia<p>Entomopathogenic nematodes Entomopathogenic nematodes (EPN) of the genera Heterorhabditis and Steinernema are commercially used to control pest insect. EPN is widely cultivated through in-vivo and in vitro methods. This research aims to discover the abundance of EPN cultivated in various artificial media. Seven types of media composition were tested in this research: media A (yeast + soybean powder), media B (yeast + chicken liver), media C (yeast + dog food), media D (yolk + soybean powder), media E (yolk + chicken liver), media F (yolk + dog food), and media G (yeast + yolk + dog food). Each media was inoculated in 1.2x103JI/mL. The growth of EPN was observed weekly in 4 weeks. Results showed that EPN could be cultivated using various media; media D, E, F, and G. Highest abundance of EPN is found in the second week of media D for 28164 JI/ml.Cell harvesting is suggested to be conducted during the first and second week to obtain maximum abundance of EPN.</p><p><strong>How to Cite</strong></p><p>Indriyanti, D., & Muharromah, N. (2016). Mass Cultivation of Entomopathogenic Nematode In Artificial Media.<em> Biosaintifika: Journal of Biology & Biology Education</em>, 8(1), 111-118.</p>https://journal.unnes.ac.id/nju/index.php/biosaintifika/article/view/5579cultivation mediaEntomopathogenic Nematodein-vitro |
| spellingShingle | Dyah Rini Indriyanti Nur Lailatul Muharromah Mass Cultivation of Entomopathogenic Nematode in Artificial Media Biosaintifika: Journal of Biology & Biology Education cultivation media Entomopathogenic Nematode in-vitro |
| title | Mass Cultivation of Entomopathogenic Nematode in Artificial Media |
| title_full | Mass Cultivation of Entomopathogenic Nematode in Artificial Media |
| title_fullStr | Mass Cultivation of Entomopathogenic Nematode in Artificial Media |
| title_full_unstemmed | Mass Cultivation of Entomopathogenic Nematode in Artificial Media |
| title_short | Mass Cultivation of Entomopathogenic Nematode in Artificial Media |
| title_sort | mass cultivation of entomopathogenic nematode in artificial media |
| topic | cultivation media Entomopathogenic Nematode in-vitro |
| url | https://journal.unnes.ac.id/nju/index.php/biosaintifika/article/view/5579 |
| work_keys_str_mv | AT dyahriniindriyanti masscultivationofentomopathogenicnematodeinartificialmedia AT nurlailatulmuharromah masscultivationofentomopathogenicnematodeinartificialmedia |